1. Microscopy The discipline of science which involves magnifying objects which cannot be seen with the naked eye is known as microscopy. 2. Fluorescence- an introduction Certain substances, when irradiated with light (UV, blue or green) emit radiations of their own, whose wavelength is longer than that of the exiting light. This process is called luminescence.
The results of the motility testes were as follow: Unknown 1- Negative Unknown 2- Positive Unknown 3- Positive Unknown 1 was negative in motility as the bacteria only remains on the surface of the stab culture media, the bacteria do not spread along the stab in the media. Whereas the Motility of unknown 2 and 3 were positive as it does not just spread on the surface of the media, it also spread along the stab in the media. Hence positive. Figure 9. Microbact Kit (before add.
With help from the beam of electrons, the electron microscope has the ability to expose the structure of the object itself. For example, an atom could be clearly seen (with full details) with this high technical electron microscope. The electron microscope do have a greater resolving power, which means that it’s possible to see extremely tiny objects with finer details, for example, an atom (10-10). Ordinary high technical electron microscopes are capable of a resolution of 1 nm (one nanometer = 1 x 10-9 m = 1/1,000,000,000). There are two specific variants of electron microscopes; scanning electron microscope (SEM) and transmission electron microscope
Light is passes directly through the specimen and usually requires staining. The magnification power is 400x.Lenses is very long as compare to 100 xs, 40 xs, and 10 x. BRIGHT FIELD MICROSCOPE PURPOSE Bright field microscopy formed its image when light is transmitted through the specimen. PRINCIPLE Specimen being denser and more opaque then its surroundings. Observe some of this light; the rest of the light is transmitted directly up through the ocular into the field.
If the substance is a darker color, it will absorb more light, and if it is lighter it will absorb less light. In addition, the enzyme and substrate will be tested with different levels of pH and different temperatures in order to demonstrate if the reaction will improve or not based on these
TEM Transmission Electron Microscopy Introduction Transmission Electron Microscope is a type of electron microscopy that forms detailed images called micrographs. (David B. Williams et al, 1996) states that the images form by passing a beam of electrons through a very thin slice of the area of interest. The transmission electron microscope operates on the same principles as the light microscope but uses electrons instead of light. What we see with a light microscope is limited by the wavelength of light. Transmission electron microscope use electrons as light source and their lower wavelength make it possible to get a resolution a hundred times better than with a light microscope.
Molisch Test is used to detect the presence of carbohydrate and a negative result indicates the absence of carbohydrates. In the presence of concentrated sulphuric acid the carbohydrates are dehydrated forming furfuryl derivates. Naphthol-(1), the Molisch reagent reacts with the cyclic aldehydes to form purple colored condensation products (furfuryl-diphenyl-methane-dyes) (1). A red-violet layer at the interface between the acid (bottom) and aqueous (upper) layers is a positive test for carbohydrates. Precaution steps in this test is to always remember to use a glass Pasteur pipette to add the concentrated sulphuric acid and do not use a mechanical pipettor with concentrated acids.
(1) mebo ointment provide a physiological moisture need for regeneration and repair burn wound. MEBO reduces severe pain of burns, prevents shock, and reduces dermal infections. Wound healing is promoted by prevention of dermal water loss. Additionally, MEBO shows anti-inflammatory, anti-bacterial, and analgesics effects. Mupirocin ointment :- mupirocin is an antibacterial ointment.IT works by eradicate growth of bacteria ADVANTAGES • Handling of ointments is easier than bulky liquid dosage forms.
2.4Exposure One of the most important factors in photo’s quality might be brightness. Usually, photo’s brightness is controlled bytwo setting parameters, exposure time and aperture size, in a camera and an additional parameter, ISO, for a digital camera. Exposure time means the amount of time duration for which a film/sensor is exposed. You can imagine that longer exposure time would make your photo brighter than shorter exposure time and vice versa. Also, it’s straightforward to expect the effect in linear relation.
Intra beam viewing of a point source of light produces a very small spot on the retina resulting in a greatly increased power density and an increase chance of damage. A large source of light such as a diffuse reflection of a laser beam produces light that enters the eye at a large angle is called an extended source. An extended source produces a relatively large image on the retina and energy is not concentrated on a small area the retina as in a point source. Absorption of Radiation by the Eye Certain areas of the eye absorb more light in one spectral region than in other regions. Absorption of laser radiation above a certain level leads to tissue injury.