Plasmid Dna Assay Lab Report
In this assay plasmid DNA was exposed to indoor PM samples and DNA strand breakage was quantified by gel electrophoresis technique. In this assay a specific hydroxyl radical scavenger i.e. Mannitol (4 mM) was used to protect the DNA scission and measure the role of the hydroxyl radical in the degradation of the super coiled DNA (Murrant, 2001). In this assay ¼ of filter was cut off and immersed in 1 mL phosphate-buffered saline (PBS). The samples were first ultrasonicated for 15 minutes followed by shaken gently and then vortexed for 15 minutes. This released the particulate matter (PM) from the filter substrate into the solution. PM fractions were taken into an EP (eppendorf) tube and each sample was dilutes with PBS to the required concentration. Extracts of PM was tested for their ability to damage DNA in supercoilled phage DNA. 2.3.3.1.1 Standard Procedure for Plasmid DNA assay Gel electrophoresis of plasmid DNA is a method used to separate DNA in a matrix of agarose and this technique involves the following steps (i) Preparation of Gel In this study 8% agarose gel was prepared by dissolving 0.642gm agarose in 40 ml 1X TBE (tris borate EDTA) buffer. …show more content…
The major sources of error concerning the sampling and analyses of particulate matter samples include (i) artifacts or contamination of samples, (ii) loss of collected particulate matter species during sampling or after sampling, (iii) sample handling, transport and storage, (iv) modification of samples during analyses, and (v) errors in data handling. In order to control and minimize the overall uncertainty caused by these factors, the sampling of PM and weighing of filters were carried out according to a standard operation procedure to assure high quality of sample
Unknown Culture Lab Report
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Bio 101 Lab Report
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Microorganism Lab Report
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Escherichia Colo Lab Report
308 Words | 2 PagesTransformation in bacteria usually takes place when a bacterial cell accepts strange DNA and integrates to its own DNA. The transformation normally takes place within plasmids, which are tiny circular DNA molecules that have been separate from its own chromosome. The copies of the same plasmid range from 10 to 200 copies within a cell. These copies of plasmids may multiply when the chromosome replicate or multiply independently. One plasmid has a range of 1,000 to 200,000 base pairs.
Pglo Transformation Lab Report
680 Words | 3 PagesUsing an aseptic pipet, 250 µl of transformation solution was added to each micro test tube before setting the tubes on ice. Furthermore, using a sterile loop, one colony was added to each micro test tube and placed back on the ice. Using an aseptic pipet, eight µl of pGLO plasmid was transferred to the +pGLO test tube and both tubes were placed on ice for ten minutes. In the meantime, four agar plates were labelled accordingly: LB/amp (+pGLO), LB/amp/ara (+pGLO), LB/amp (-pGLO), and LB (-pGLO). Using the foam test tube rack as a holder, both the (+) and (-) pGLO test tubes were placed in a water bath set at 42°C for 50 seconds, and then moved quickly back onto ice for two minutes.
Pglo Lab
1396 Words | 6 PagesTransformation was successful in the plates where the bacteria consumed the pGLO plasmid. In the first plate that the bacterium was plated on it included the LB broth and of ampicillin antibiotic (amp), 2 colonies were present. The second plate of bacteria was grown with the presence of LB broth, ampicillin, arabinose sugar (ara), and 22 colonies were observed. But a green fluorescent glow of the colonies was only present in plate 2. Plates 3 and 4 were the control plates.
Lab Report
1244 Words | 5 PagesST Report In the experiment, the problem was the contaminants that were affecting the quality of the water samples. To fix this issue, three scientists had to determine the contaminants that were present in the samples. One sample was from the school sink and the second sample was from an unknown source. The scientists conducted many tests to figure out what pollutants were present in the water.
Dna Fingerprinting Lab Report
1726 Words | 7 PagesDNA Fingerprinting Using Agarose Gel S. Aaron Sowards Bio 122 Lab 04 Brianna Adanitsch Jakob Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA.
Environmental Injustice In The Bronx
399 Words | 2 PagesThe Bronx is an incredibly urban and populated area attracting traffic from all over. It is home to waste sites, distribution centers, power plants, and so much more. Though each building gives off a different type of waste, whether it be smog from smokestacks or diesel exhaust from trucks, each of these large sites that exist throughout this small area give off a fine particulate matter known as PM2.5. This matter is essentially what contributes to the difficulty breathing people experience as these tiny particles can become lodged in a person’s lungs over time. Studies have consistently found a strong correlation between PM2.5 and conditions like asthma, lung disease, and heart disease.
Unit 8 Dna Research Paper
910 Words | 4 PagesDNA from the actual parents - Use these chromosomes to make a baby for the DNA profile. Sample D D 248 BP___TPOX #2 Pater. Chromo D 145 BP_D5
Pglo Transformation Lab Write Up
984 Words | 4 PagesThe purpose of this experiment was to insert the plasmid glow green into the bacteria with a gene of interest to produce the protein that make the bacteria glow green along with the presence of arabinose and the presence of ampicillin. Many scientists are experimenting different kind of genes that can inserted into the organism for survival. The technique of transformation was used in this experiment to give the organism a new trait that they did not possess in their life. In this experiment, the bacteria were added to four plates with certain conditions such as the existence of plasmid, ampicillin, and arabinose to see whether the bacteria grow and glow green. The results showed that the LB/amp/araC +pGLO produce a lot of colony and most
Blood Agar Lab Report
1057 Words | 5 PagesThe Another medium used was MAC, it is used to isolate and differentiate gram-negative organisms and it is a pink, dusty rose color. Lastly, the Citrate Slant is a green color and it was used as a differential test to examine enzymes. The media were inoculated at 37°C for 48 hours, then it was observed to determine the
Deoxyribose Lab
2057 Words | 9 PagesThe DNA gathered by the group bore positive results only on Test for Deoxyribose; compared to the standard solution, which bore positive results on all chemical tests, namely, Test for Deoxyribose, Test for Phosphate, Test for Purines, and test for Pyrimidines. Introduction Nucleic Acid is one of the essential biochemical molecules
Biochemical Test Lab Report
1371 Words | 6 PagesBiochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
Prokaryotic Cell Lab Report
3367 Words | 14 PagesProkaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan.
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