Probiotic Bacteria
Probiotics are a group of bacteria described by the Food and Agriculture Association of the United Nations (FAO) and World Health Organization (WHO) as live microorganisms which confer a health benefit on the host when administered in adequate amounts . Dairy Federation has recommended that the bacteria be viable and abundant in the product population of at least 107 colony-forming units (CFU/g) until the date of consumption. However, studies indicate that the bacteria may not survive in sufficient numbers when incorporated into food products and during their passage through the gastrointestinal tract . Several factors influence the survival and colonization of these bacteria, including resistance to low pH, bile acids, and digestive enzymes …show more content…
The increased cationic nature of chitosan relative to alginate may explain this increase in protection. Chitosan coating compare to alginate alone gave greater survival levels. Moreover, this protective effect was not influenced by fluid bed drying method . added galactooligosaccharides (GOS) and inulin during microencapsulation of Lactobacillus acidophilus 5 and Lactobacillus casei 01 in alginate beads coated with chitosan at the concentrations of 0, 0.5, 1.0 and 1.5%. The presence of GOS (0.3%) in the microencapsulation provided the best protection with only 3.1 and 2.9 logs reduction for L. acidophilus 5 and L. casei 01, respectively, after incubation in simulated gastric juice (pH 1.55), followed by simulate intestinal juice containing 0.6% bile salt. The aim of this study was to investigate the prebiotics effect of inulin-type fructans with different chain lengths (DP<10, ~ 12 and ≥ 23) on the morphology, particle size and survival of microencapsulated probiotics strain of Lactobacillus casei in alginate beads, alginate coated with chitosan, alginate-inulin beads and alginate-inulin coated with chitosan exposed to simulated gastrointestinal
Unknown Microbial # 398
532 Words | 3 PagesMethods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.
Hunting Nightmare Bacteria Case Study
986 Words | 4 PagesHunting nightmare bacteria Answer the following questions Case of Addy (the girl from Arizona ) 1- Based on the pediatrician observations what was Addy’s diagnosis at the Pediatric Hospital intensive care unit ? She had got infected by staff or positive bacteria called Methicillin-resistant Staphylococcus aureus (MRSA). MRSA is a community associated bacteria that infects kids when they are playing in playing ground and getting scabs on their knee. They spread through that wound and it has very high resistance to antibiotics.
Unknown Bacteria Lab Report
1200 Words | 5 PagesThe purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
Clostridium Difficile In Hospitals
1149 Words | 5 PagesGastroenterology Research & Practice, 1-6. doi: 10.1155/2016/2687605 Nanwa, N., Sander, B., Krahn, M., Daneman, N., Lu, H., Austin, P., Govindarajan, A., Rosella, L., Cadarette, M., & Kwong, J. (2017). A population-based matched cohort study examining the mortality and costs of patients with community-onset clostridium difficile infection identified using emergency department visits and hospital admissions. Plos ONE, 12(3), 1-13. doi:10.1371/journal.pone.0172410 Ng, K., Ferreyra, J., Higginbottom, S., Lynch, J., Kashyap, P., Gopinath, S., Naidu, N., Choudhury, B., Weimer, B., Monack, D., & Sonnenburg, J. (2013). Microbiota-liberated host sugars facilitate post-antibiotic expansion of enteric pathogens.
Imagining The Post Antibiotics Future Summary
963 Words | 4 PagesIS THE POSSIBILITY OF POST-ANTIBIOTICS REAL? Imagining the Post-Antibiotics Future is an essay written by Maryn McKenna to emphasize focus on our lack of appreciation for what antibiotics have done for us and will continue to do for us, but only if we let them. She presents a very insightful and eye opening argument. She relies heavily on a very personal story as well as many facts and research to create such a convincing argument. McKenna begins her essay with recalling a time in which she found out about the death of her great-uncle due to a very infection.
Unknown Lab Report
1444 Words | 6 PagesA starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids. The smoke of the Iodine stained the plate to display the presence or absence of a halo around the bacteria 2.12 Lipid Hydrolysis This test was done by making a single line streak inoculation on a tributyrin agar plate and allowing incubation. After the incubation period, the plate was observed for the presence or absence of a halo around the bacteria.
Unknown Bacteria Lab Report
1034 Words | 5 PagesIntroduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans).
Missing Microbes Summary
844 Words | 4 PagesIn the book Missing Microbes, the author, Dr. Martin J. Blaser discusses different types where the mysterious microbes are to be found. Dr. Martin also discusses his hypothesis in which talks about how over use of antibiotics has permanently changed the microbiome that humans live in, causing an increase in more modern diseases. The way Blaser lays the book is more like a journey; he traces his footsteps, and has the readers following the lead anxiously waiting on what he will inform them. There are a lot of doors in Science. Dr. Blaser chose to enter the door where facts and stories are to be learned everyday, in which there is no end, making that the beauty of science.
Unknown Bacteria
557 Words | 3 PagesIn the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Unknown Bacteria Report
884 Words | 4 PagesWhen given an unknown bacteria there are a multitude of steps one must go through to be able to correctly identify what bacteria was given. It is important to correctly identify the bacteria because some bacteria are more harmful than others. The gram stain is the first test that should be performed because it helps narrow down the possibilities by telling one whether the bacteria is gram positive or gram negative. After this test is performed, one shall place bacteria on/in Mannitol Salt agar, MacConkey agar, Eosin Methylene Blue agar, Urea agar, Simmon’s Citrate, Purple Beef broth with Lactose and finally Purple Beef broth with Sucrose. A streak plate should also be made up, this helps one identify the morphology of the colonies.
Microbes In Deadly Companions
641 Words | 3 PagesIn the first four chapters of Deadly Companions author Crawford takes us on a journey on how microbes shaped our history. Starting with when microbes first appeared on planet Earth to Darwinian evolution of single celled organism. In chapter two “Our Microbial Inheritance” she discussed the relationship of microbes with hunter-gatherers and early settlements; and their relationship to disease. She looks at the impact of microbes on the lives of individuals and the population as a whole. Hunter-gatherers were small groups of people that hunted for food, moving from place to place.
E. Coli Research Paper
1004 Words | 5 PagesEscherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge
Biochemical Test Lab Report
1371 Words | 6 PagesApproximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only
Advantages And Disadvantages Of Chitosan
1700 Words | 7 PagesUsing chitosan as a food preservative allows for reuse of this waste, making its use environmentally beneficial; while also reasonably cheap and cost-effective in production and usage. - Its biodegradability also allows chitosan biofilms to be beneficial to the environment. - The chitosan film has antibacterial and antifungal properties, meaning that it ultimately prolongs shelf-lives of the foods which it coats. - As chitosan film is a polysaccharide which physically coats the food product, it offers the advantage of being edible,
Prokaryotic Cell Lab Report
3367 Words | 14 PagesProkaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan.
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