The Pros And Cons Of Counting The Bacteria
Counting the bacteria means determining the number of bacteria in a specific sample. There are many ways that we can use to count the microorganisms, one of which is the plate count. The plate count technique is used to count only the living bacteria by counting the number of colonies.Due to the large number of bacteria, and to be able to count it, the bacteria should be diluted several times and spread on an agar plate. In this way, colonies can be counted. These colonies are called colony forming unit (CFU). This method has many limitations since it only shows the number of living bacteria. Also, due to the different arrangements, not all colonies consists of a single bacterium. However, with calculations we can count the number of single
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839 Words | 4 PagesBacteria are living things that have only one cell. Under a microscope, they look like balls, rods, or spirals. Many are helpful. Some bacteria help to digest food, destroy disease-causing cells, and give the body needed vitamins. Bacteria are also used in making healthy foods like yogurt.
Hunting Nightmare Bacteria Case Study
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Unknown Culture Lab Report
1054 Words | 5 PagesAfter 5 days the plates were removed from the cold room and the gram-negative test for Colony A on the EMB agar showed pink fisheye colonies which lead to the conclusion that the gram-negative organism within Unknown #21 was Enterobacter aerogenes. Had the pigmentation been metallic green, the organism would have been identified as Escherichia coli, and had there been no pigmentation at all a Triple Sugar Iron agar (TSI) test among other tests would have been
Unknown Bacteria Lab Report
1200 Words | 5 PagesDifferential media allows for the differentiation between two similar micro-organisms through how the bacteria may handle certain compounds found in the media or the different reactions that may take place when the bacteria is exposed to the medium (3). Selective media on the other hand allow only certain microbes to grow. This is due to the plate containing a limited amount of nutrients, compounds and chemicals that will deter the growth of certain bacteria (3). Dyes, antimicrobial substances, salts, certain growth inhibitors and, antibiotics are also found on this type of medium (3). The differential and selective media mentioned in this lab are as follows:
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1596 Words | 7 PagesI expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided
Pglo Lab
1396 Words | 6 PagesTransformation was successful in the plates where the bacteria consumed the pGLO plasmid. In the first plate that the bacterium was plated on it included the LB broth and of ampicillin antibiotic (amp), 2 colonies were present. The second plate of bacteria was grown with the presence of LB broth, ampicillin, arabinose sugar (ara), and 22 colonies were observed. But a green fluorescent glow of the colonies was only present in plate 2. Plates 3 and 4 were the control plates.
Unknown Lab Report
1444 Words | 6 PagesThese microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal
Unknown Microbiology Essay
517 Words | 3 PagesBeing able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Unknown Lab Report Essay
408 Words | 2 PagesThe unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
The Pros And Cons Of Microorganisms
966 Words | 4 PagesMicroorganisms- the smallest organism’s that are absolutely impossible to observe through our naked eyes. The solution to overcome this problem is by using the microscope. A microscope is an instrument that is used to see objects that are too small for the naked eye.
Exercise 14 Unknown Identification Lab Report
449 Words | 2 PagesExercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
Biochemical Test Lab Report
1371 Words | 6 PagesBiochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
Hand Washing Persuasive Speech
877 Words | 4 PagesINTRODUCTION: Quick look at your hands do you see them. (attention) Do I see what you might be asking? Well the millions of bacteria that are currently hanging out on your hands.
Agglutination Test Lab Report
1772 Words | 8 PagesThis can be tested by simply mixing the serum of suspected individual which contain the antibodies with the antigens of specific bacteria the accumulation of clumps confirms the presence of particular bacterial infection.[2] This test can be performed in various ways including slide agglutination reaction, tube agglutination reaction, indirect agglutination inhibition reactions etc. Another important practical application involves blood group test of
Cell Viability Test Lab Report
1000 Words | 4 PagesExperiment 3: Cell Viability Testing and Counting with Trypan Blue Exclusion Method Anna Coretta R. Santos I. Introduction Cell viability assay plays a crucial role is in the cells of the cell culture. This assay can be used to identify the relationship of cell count from the cell’s behavior (Stoddart 2011). This test help researchers to determine the number of viable and non-viable cells in a cell culture. This assay helps in estimating the cell count of a cell culture and to determine if there is sufficient amount of viable cells in the cell culture that a person will use for future research (Riss et al. 2013).
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