Thermal Oven Lab Report

852 Words4 Pages
• Thermal Oven
• pH analyzer
• Triple Quartz Distillation Unit
• HPLC Injecting Syringe (25 mL) (HAMILTON)
REAGENTS USED
1. Acetonitrile HPLC grade
2. Methanol HPLC grade
3. Water – HPLC grade
4. Mixed Phosphate Buffer
5. Lamivudine

LAMIVUDINE

TRAIL METHOD-I

Mobile phase Acetonitrile : Water (50:50) λmax 270 nm
Flow rate 1mL/min
Column Inertsil C8 150 x 4.6 mm
Retention Time 1.800 min
Efficiency 2030

Preparation of mobile phase:
Take 100ml of Acetonitrile in volumetric flask and dissolve it in 100ml of water. Then it is sonicated it for 10mint.

TRAIL METHOD-II

Mobile phase Water : Menthol (40:60) λmax 270 nm
Flow rate 1mL/min
Column Inertsil C8 150 x 4.6 mm
Retention Time 1.783 min
Efficiency 1294

Preparation of mobile phase
…show more content…
Then it is sonicated it for 10mint.
TRAIL METHOD-III

Mobile phase Mixed Phosphate Buffer (55:45) λmax 270 nm
Flow Rate 1ml/min
Column Inertsil C8 150 x 4.6 mm
Retention Time 1.567 min
Efficiency 999

Preparation of mobile phase Take 16.25gm of potassium dihydrogen phosphate and 0.3gm of dipotassium phosphate in volumetric flask and make volume up to 550ml by adding phosphate buffer to it , 450ml of Acetonitrile is added to the above one and made volume to 1000ml. Then it is sonicated for 10mint.

TRAIL METHOD-IV

Mobile phase Mixed Phosphate Buffer (55:45) λmax 270 nm
Flow Rate 1ml/min
Column Inertsil C18 250 x 4.6 mm
Retention Time 2.713 min
Efficiency

More about Thermal Oven Lab Report

Open Document