Thermal Oven Lab Report
• Thermal Oven
• pH analyzer
• Triple Quartz Distillation Unit
• HPLC Injecting Syringe (25 mL) (HAMILTON)
REAGENTS USED
1. Acetonitrile HPLC grade
2. Methanol HPLC grade
3. Water – HPLC grade
4. Mixed Phosphate Buffer
5. Lamivudine
LAMIVUDINE
TRAIL METHOD-I
Mobile phase Acetonitrile : Water (50:50) λmax 270 nm
Flow rate 1mL/min
Column Inertsil C8 150 x 4.6 mm
Retention Time 1.800 min
Efficiency 2030
Preparation of mobile phase:
Take 100ml of Acetonitrile in volumetric flask and dissolve it in 100ml of water. Then it is sonicated it for 10mint.
TRAIL METHOD-II
Mobile phase Water : Menthol (40:60) λmax 270 nm
Flow rate 1mL/min
Column Inertsil C8 150 x 4.6 mm
Retention Time 1.783 min
Efficiency 1294
Preparation of mobile phase
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Then it is sonicated it for 10mint.
TRAIL METHOD-III
Mobile phase Mixed Phosphate Buffer (55:45) λmax 270 nm
Flow Rate 1ml/min
Column Inertsil C8 150 x 4.6 mm
Retention Time 1.567 min
Efficiency 999
Preparation of mobile phase Take 16.25gm of potassium dihydrogen phosphate and 0.3gm of dipotassium phosphate in volumetric flask and make volume up to 550ml by adding phosphate buffer to it , 450ml of Acetonitrile is added to the above one and made volume to 1000ml. Then it is sonicated for 10mint.
TRAIL METHOD-IV
Mobile phase Mixed Phosphate Buffer (55:45) λmax 270 nm
Flow Rate 1ml/min
Column Inertsil C18 250 x 4.6 mm
Retention Time 2.713 min
Efficiency
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