Based on the assumed contamination of the TLC plate and or capillary tube, it is not possible to tell whether acetaminophen was successfully separated from the Excedrin powder. The Rf values of isolated aspirin and pure aspirin were the same. This demonstrates that the aspirin was successfully separated and is relatively pure. The isolated caffeine sample had a higher Rf value but when viewed under UV light, the markings of isolated caffeine were within the bounds of the pure caffeine, leading to the conclusion that while isolated the caffeine sample was note
Therefore, liquid-liquid and acid-base extraction techniques were successfully performed to separate the components of the Excedrin tablet. According to the TLC analysis results, the compounds (aspirin, acetaminophen, and caffeine) were successfully isolated from the analgesic (Excedrin tablet). In figure 1, the separation of the compound in the TLC analysis correlates with the TLC analysis in figure 2. Furthermore, Rf index calculations of the TLC analysis demonstrated that the compounds (aspirin, acetaminophen, and caffeine) were separated. The Rf calculations of aspirin in table 1 shows an Rf value of .491; however, in table 2 the Rf value of aspirin was calculated to be .784.
After finding the Rf values of the four known compounds, solvent 1 (99.5% ethyl acetate/0.5% acetic acid) was chosen, due to the wide range of results, for the remaining experiments. Ibuprofen, our known tablet, gave a similar Rf value to our previous results for Ibuprofen. For Anadin extra, there were three compounds identified as Caffeine, Paracetamol and Aspirin as the Rf values of the drug were close to the values of these three compounds in the first part of the practical. For both of these known drugs, the Rf values acquired were close to my predictions before the experiment. For the unknown powder, we obtained Rf values of 0.52 and 0.76 so we believe that the unknown powder contains Aspirin and Ibuprofen.
Next, the test tubes were carefully cleaned with soap and water. Then five millilitres of sample “A” was placed in the test tube labeled “A”. This was then repeated with the next three samples. Then a few drops of each sample were placed on glucose/ketone paper. Each piece of glucose/ketone paper (with the sample on top) was compared to the label on the glucose paper bottle.
Experiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated.
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
Audience: This paper is written with the intent of addressing an audience consisting of advanced high school students and lower division college students, who are interested in learning what happens to their bodies internally when ibuprofen is consumed. Students should have taken a general chemistry, and biology course as a prerequisite. It is expected that the student have some previous knowledge on nomenclature, spectroscopy, and basic internal organs.
TLC was used to identify the actual unknown product as well as other products/reactants present in the filtered solution. The procedure was conducted by placing a TLC plate in a developing chamber that is filled with a small amount of solvent. The solvent cannot be too polar because it will cause spotted compounds on the TLC plate to rise up too fast, while a very non-polar solvent will not allow the spots to move. The polarity of the spots also determines how far it moves on the plate; non-polar spots are higher than polar ones. After spots on the TLC form, the Rf values are calculated and used to analyze the similarity of the compounds.
This study was conducted with a partner, since some parts of the experiment were able to be done simultaneously. One partner prepared a TLC developing jar by pouring a small layer of 4:1:1 propanol/acetic acid/water into a developing jar. A solvent wick was made by wetting a piece of filter with the solvent, and it was placed in the jar. A silica coated TLC plate was obtained, and a spotting line was carefully drawn approximately 1.5 cm from the bottom of the plate using a pencil. Extra care was taken to not touch the plate with bare skin.
The purpose of this experiment was to isolate the three components of Excedrin using column chromatography. Thin layer chromatography (TLC) was used to determine when each of the components had been fully eluted from the column. If there was an overlap in fractions between two components, liquid- liquid extraction was done to separate them. The compounds were characterized via NMR instrumentation and the percent recovery for each compound was calculated to determine whether the isolation was
INTRODUCTION A gas chromatograph (GC) can be utilized to analyze the contents of a sample quantitatively or in certain circumstances also qualitatively. In the case of preparative chromatography, a pure compound can be extracted from a mixture. The principle of gas chromatography can be explained as following: A micro syringe is used to inject a known volume of vaporous or liquid analyte into the head or entrance of a column whereby a stream of an inert gas acts a carrier (mobile phase). The column acts as a separator of individual or chemically similar components.
Lab Report 5: Acetylsalicylic Acid (Aspirin) Synthesis Name: Divya Mehta Student #: 139006548 Date Conducted: November 19th 2014 Date Submitted: November 26th 2014 Partner’s Name: Kirsten Matthews Lab Section: Wednesday 2:30 L9 IAs Name: Brittany Doerr Procedure: For the procedure, see lab manual (CH110 Lab Manual, Fall 2014) pages 96-98. Wilfrid Laurier University Chemistry Department. Fall 2014. Acetylsalicylic Acid (Aspirin) Synthesis.
These prostaglandin chemicals are produced by enzymes called cyclooxygenases (COX). The job of the NSAID is to block the COX enzymes, thus prohibiting it from producing prostaglandins and therefore inflammation is reduced. The purification of the unknown will be done through a process called flash chromatography, a microscale version
Introduction The term chromatography actually means colour writing, and signifies a technique by which the substance to be examined is placed in a vertical glass tube containing an adsorbent, the different segments of the substance traveling through the adsorbent at distinctive rates of velocity, according to their degree of attraction to it, and producing bands of colour at different levels of the adsorption column. The substances least absorbed emerge earliest; those more strongly absorbed emerge later. (Wixom et al., 2011) In chromatography of all types, there is a mobile phase and a stationary phase.
In this lab, three unknown compounds were separated from a mixture and identified by melting point. Unknown mixture #124 has components of acid, base and neutral compound. The compounds were identified by melting point and matched up with the known melting points from a given list. In order to identify the compound it was important to separate by dissolving the mixture in an organic solvent which was not soluble in water, and then extracting the solution first with HCl, and then dilute sodium hydroxide solution. From the separation mixture, the aqueous layer were obtained and labeled as TT-1 (base), TT-2(acid) and TT-3 (neutral) in three different test tubes for later recovery.