Stored in light glass bottle. 2.5.2: Formation of PANI(CoFe2O4) 0.1g of Nano material and 50ml of Aniline hydrochloride were mixed in a beaker. Then 50ml of Ammonium peroxydisulphate was added drop wise in reaction mixture with constant stirring below 20 oC. After 24 hours the coated sample was filtered, washed and dried at 60 oC in oven and then grinded into a fine powder in agate
Throughout the mixing process, the clear red solution slowly changes to a denser red solution (Appendix figure 23). A thermometer was used for temperature checking. The beaker was removed from the hot plate when the temperature was found to be higher than 50 ℃. This was done to prevent a sudden gelation happen before all the active dissolved in the ethylene glycol. Moderate heating of the solution for a period of time is allowed to obtain a wet gel (Appendix figure 24).
The mixture was placed in a water bath (90ºC) for 10 minutes to allow the reaction to be completed. After the reaction has being completed, the test tube was removed and allowed to be cooled. The alcohol (methyl alcohol) loses OH while the carboxylic acid (salicyclic acid) loses a H. The method which is used in this experiment is only suitable for preparing small amounts of ester. The ester which is formed also can only be categorized by odour. After it has cooled, the fumes was carefully walf by passing our hand over the top of the tube towards our
Added 2 ml of the dissolved sodium hydroxide into the heated oil, immediate the mixture turns cloudy. Set aside to stir for 30 minutes on high. Transfer contents of beaker into a 250 mL separator funnel. Leave the mix to break up for overnight. Transfer the glycerol (bottom layer) into a beaker from
The mixture was then distilled. When the temperature was reached to about 59℃, half vial of distillate (1V) and 1 mL of the liquid residue (1L) were collected. For 61.0℃, the distillation was then continued. Samples (2V, 2L) were taken at about 61.0℃. For 63.0℃, the flask was cooled when the solution in the flask become almost empty.
1 ml of the extract and Piperine was dissolved in ethanol and added to the polymer solution along with propylene glycol and methyl paraban with gentle stirring. Few drops of triethanolamine was added and mixed well which result in the formation of Myristica fragrans gel. Table 1: Composition of topical gel formulation S. NO INGREDIENTS MF1 MF2 MF3 1 Myristica fragrans extract
General procedure for synthesis of 2-amino-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carbonitrile. To a solution of cyclohexanone (2.94 g, 30 mmol), malononitrile (1.32 g, 20 mmol), and elemental sulfur (0.64 g, 20 mmol) in 20 mL EtOH, piperidine (0.85 g, 10 mmol) was slowly added and the temperature was kept under 50 ᵒC. The reaction mixture was heated under reflux for 2 h and was left to cool down overnight. The precipitated product was filtered and washed with cold ethanol (2 x 10 mL). Brown crystals product; mp 143 ᵒC was crystallized from ethanol.
Step-III: Synthesis of Cr(II) Complexes: The Schiff's base complexes were synthesized by mixing the Schiff's base (1.5 g) in ethanolic solution of Chromium chloride [CrCl2]. This reaction is refluxed in a waterbath for two hours and their volume were reduced to 70% of it’s original volume and residue was obtained. The coloured product obtained was filtered under suction, washed with ethanol. The product were recrystallized from ethanol. Their yields ranges from 50-55%, the product obtained were light green colour and melting point was 2100C.
One millilitreer of the lichen extract (1 mg/mL) in a volumetric flask was diluted with distilled water (46 mL), and the content was mixed in a volumetric flask after adding. oOne millilitreer of Folin-Ciocalteu reagent was added and the content of the flask was mixed. thoroughly . After 3 min, 3 mL of 2% sodium carbonate (2 %) was added and then was allowed to standleft for 2 h with intermittent shaking. The reaction absorbance of prepared mixture absorbance was measured at 760 nm in a spectrophotometer (Jenway UK).