Thymol Lab Report

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SYNTHESIS,CHARACTERIZATION AND BIOLOGICAL ACTIVITES OF 2-FORMYLTHYMOL AZOMETHINES LEENA N.PATIL Department of Engineering Sciences and Humanites, Sandip Institute of Engineering and management, Sandip Foundation , Trimbak road, Mahiravani,Nashik-422213 E mail:- leena.patil@siem.org.in ABSTRACT: Thymol is a naturally occurring phenolic monoterpenoids which possesses ecological role and broad spectrum of biological activities. Hence thymol was ortho formylated to 2-formyl thymol and further derivatised to it’s azomethines derivatives. It is characterized by IR Spectroscopy, NMR Spectroscopy and Mass Spectroscopy. Microbial activity for A. niger species was investigated . The antifungal activity was found to increase with derivatization. KEY…show more content…
The temperature inside the flask was adjusted to 60-65 0C ) chloroform (3.25mL, 0.04M) was introduced in small portion with stirring by maintaining the temperature to 60-65 0C during addition. The mixture was stirred for 2 hours. Excess of chloroform was removed by steam distillation from alkaline solution. The solution was allowed to cool and then it was acidified with dilute HCl (1%). The acidified solution was steam distilled until no more oily drops were collected. The distillate was extracted with ether (3x30mL). Most of ether was removed by distillation. The residue, which contains thymol and thymolaldehyde was transfered to a small glass stoppered flask and about twice the volume of saturated sodium metabisulphite solution was added. The solution was stirred vigoursly for ½ h and allowed to stand for 1 h. The paste of bisulphite compound was filtered and washed with little ethanol and finally with little ether. The bisulphite compound was decomposed by warming in round bottom flask on a water bath with dilute H2SO4. The mixture was allowed to cool and then the thymolaldehyde was extracted with ether (3x30mL). Removal of ether gave pure thymoladehyde…show more content…
As the number of aromatic rings were increased, the activity of azomethine derivatives also increased. Introduction of methylene with phenyl group showed enhanced in activity. Addition of o-hydroxy phenyl group to o-thymolaldehyde also increased the activity. The antifungal activity was observed to decrease with decrease in number of methylene moiety in N-(hydroxy alkyl) azomethine derivatives of o-thymolaldehyde. 2,2’-{hexylene-1,6-di-[(imino)methyl]}-bis-[6-isopropyl-3- methylphenol] azomethine derivative of o-thymolaldehyde (3g) showed comparable antifungal activity with carbendazim (standard) (26.66 ± 1.53) against A. niger. REFERENCES:- 1) Carson R., Silent Spring, Houghton Boston, Massachussets. (1962). 2) Edward C.A., Environmental Pollution by Pesticides, Plenum Press.(1973). 3) Geroghiou G.P. Taylor C.E., Pesticide Resistance: Stratergies & Tactis for Management, National Academic Press, Washington D. C. (1986) 157-169. 4) Pimental D., Andow D., Dyson-Hudson R., Environmental and Social Costs of Pesticides- a prelimanary assessment, Oikos. 34 (1980)

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