Blood Sugar Concentration

Role of Insulin in Regulating Blood Sugar Concentration in Tinospora cordifolia Treated Albino Rats
Kinkar Shobha B. and Patil Kishor Gopal*

Department of Zoology, Government Institute of Science R. T. Road Civil Lines Nagpur (M.S.) India PIN-440 001
*Corresponding Author E-mail- [e-mail]
ABSTRACT
Insulin levels in normal control, diabetic control and Tinospora cordifolia extract treated albino rats is investigated. Healthy animals (9 months old) of both the sexes, weighing 150-190gm were used for experimentation. Rats were treated with Tinospora cordifolia whole plant extract 20ml/kg body weight twice a day from day 2 to 30. The serum insulin level decreased significantly in the alloxan treated group was noticed. In the Tinospora

In 1910, Edward Albert Sharpey-Schafer hypothesized that diabetes was due to the deficiency of a single chemical produced by the pancreas; he called this chemical insulin, from the Latin word insula, meaning island and referring to the pancreatic islet cells of Langerhans. In 1921, Banting and Best actually discovered insulin when they reversed diabetes that had been induced in dogs with an extract from the pancreatic islet cells of healthy dogs; together with Collip and Macleod, they purified the hormone insulin from bovine pancreases and were the first to use it to treat a patient with diabetes (Banting et al., 1991 and Bliss 2007).
The production of insulin and its therapeutic use quickly spread around the world. This series of events may be the most dramatic example of the rapid translation of a discovery in basic science into a benefit for patients. Once insulin injections became available, young people with insulin deficiency who had previously faced almost certain, painful death within weeks to months were able to survive for prolonged periods of

Animals were free to access drinking water and food. Animals were cared for and used in accordance with the Institutional Animal Ethics Committee (IAEC), P.G.T. Department of Zoology, RTM Nagpur University, Nagpur (Registration number- 478/01/a/CPCSEA). For experimental induction of diabetes, alloxan monohydrate (A7413 Sigma Aldrich) was used. 180 mg/kg intraperitoneal dose of alloxan monohydrate was used for diabetes induction with the 6-12 month old rats. For this study rats were divided into three groups (n=6),
Group-I (NC): Kept as normal control (NC) the animals of this group was free to access drinking water and food they neither injected by alloxan nor fed on plant extract.
Group- II (DC): These group animals were injected with alloxan monohydrate (180 mg / kg bw) and kept as diabetic control. They were not fed on