Tris Succinate Puffer

PRACTICAL REPORT - PRACTICAL 2 AND 4 BCM367

Name: Maricell Jones Mark:

Student number: 15139931

INTRODUCTION

AIM

During Practical 2 and 4, there were two separate, but intercalated aims for the experiment. To create a stock solution of the Tris succinate or Tris (hydrodymethyl) aminomethane succinate buffer (practical2) and to create a PH range of this buffer from the stock solution for use in experiments later in the course (practical 4).

BACKGROUND INFORMATION

For practical 2, the preparation of an accurate stock solution in a quick and reliable manner is of utmost importance. The preparation of buffers is also important for any basic biochemistry. In essence, a stock solution is a prepared solution, where the

A pH meter is a tool used to measure the hydrogen-ion activity in water-based solutions. Thus the acidity or alkalinity is expressed as pH. The pH meter measures the electrical potential difference between the pH electrode and a reference electrode and is thus also revered to as a "potentiometric pH meter"². This tool is important in both practicals, as it monitors the correct

In practical 4, a titration curve is set up to determine the amount of the acid or base that needs to be added to the stock solution prepared in practical 2, to reach the wanted pH. A titration curve is used often to record titrations. They are represented visually by graphs, called titration curves, which generally contain the volume of the titrant as the independent variable and the pH of the solution as the dependent variable² (because it changes depending on the composition of the two solutions). Figure 3²: An example of a titration curve.

When the titration curve is set up, the wanted pH is extrapolated and the difference between the wanted pH and the current pH are corrected. To move the curve to the left, acid needs to be added, and in the case of experiment 2, HCl was used. To move the curve to the right, base is added, NaOH, in this case. Another important note is the equivalence point (where all of the starting solution (usually an acid) has been neutralized by the