Faculty of Pharmacy
Analytical Chemistry Department
Assignment Topic: Calcium Acetate
Course Title: Analytical Chemistry 2
Course Code: PC223
Lab Group: F3
Submitted To: Dr. Souha Hosam
Prepared By: Manar Mohamed Abd El Aziz - 170339
Due Date: 23-10-2017 Introduction:
There is more than one method to be used in the quantitative analysis. One of these methods is titration method which used in the titrimetric analysis. Titrimetric analysis is the quantitative chemical method which determines the accurate concentration of sample to react with another known volume and concentration substance. Titrimetric analysis was been called volumetric analysis as it expresses the analysis method better than volumetric analysis. The standard in titrimetric analysis is called titrant and the unknown concentration substance is called sample. The glassware remained attached to the term volumetric such as volumetric flasks. Titration can be defined as the process of adding the titrant to the sample until end of the reaction. The equivalence point, which also called stoichiometric point, is the point at which the reaction is end and it can be determined by observing a change in physical properties of the sample itself or by indicator which is a reagent changes its colour by the change in pH. The point at which the indicator changes its colour called endpoint. By determining the volume of the titrant and sample, the concentration will be easily calculated.
Titration can be done by many
Question3: Experiment 3 The unknown acid sample was 1 • Monoprotic Acid Trails Initial NaOH solution (mL) final NaOH solution (mL) The volume of NaOH to titrate the acid (mL) Amount of Unknown Acid sample 1 (g) The moles of the Unknown Acid (mol) Molar mass of the Unknown Acid (g/mol) A 3.38 28.31 24.93 0.150 0.0026 57.69 B 0.18 29.32 29.14 0.175 0.0029
B) Briefly describe each method, including the specific types of scientific tools and equipment used. Modified Mercalli Intensity Scale:
The colour of each test tube was recorded and if proteins were present that was recorded for each test tube. Finally, the pH was recorded for each sample using pH
Identification of an Unknown Compound using Quantitative and Qualitative Analysis Lauren Tremaglio Chemistry 1011 Lab, Section 16 Instructor: Steven Belina October 3, 2014 Our signatures indicate that this document represents the work completed by our group this semester. Experimental Design and Discussion of Results The objective of this experiment was to identify an unknown compound through quantitative and qualitative analysis. In order to find the identity of the unknown compound, an initial qualitative test for solubility was performed.
More changes followed in the methodology on the NCVS as an approach to solidify its accuracy by implementing new sampling methods and computer based technology for increased flow and confidentiality of
Hypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out. Independent Variable: Substrate concentration Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube.
Research Question: To investigate and compare how different temperature (5℃, 15℃, 25℃, 35℃, 45℃) can affect the concentration of carbon dioxide in soda water through titration with sodium hydroxide solution. Introduction: Carbon dioxide plays an important role in soft drinks. Soda water is manufactured by pumping carbon dioxide into water under high pressure. Carbon dioxide dissolves in water to form carbonic acid, which is the fizz we find in soft drinks. CO2 + H2O ⇌
1.1 Abstract The purpose of quantitative analysis of protein using a spectrophotometer is to measure the concentration of proteins in a given sample. The experiment is conducted by laboratory method (Biuret Test) and using spectrophotometer to analyze the absorbance of reactants at 540 nm, hence determining the concentration of the proteins in a given sample. The purpose of stopped enzyme assay to study B-galactosidase is to determine the effect of temperature and concentrations of substrate on enzyme activity.
INTRODUCTION A gas chromatograph (GC) can be utilized to analyze the contents of a sample quantitatively or in certain circumstances also qualitatively. In the case of preparative chromatography, a pure compound can be extracted from a mixture. The principle of gas chromatography can be explained as following: A micro syringe is used to inject a known volume of vaporous or liquid analyte into the head or entrance of a column whereby a stream of an inert gas acts a carrier (mobile phase). The column acts as a separator of individual or chemically similar components.
The objectives of this experiment were to use knowledge of chemical formulas and chemical nomenclature to experimentally determine the empirical formula of copper chloride. Common laboratory techniques were used to conduct a reaction between copper chloride and solid aluminum in order to get rid of the water of hydration. The amount of water of hydration in the sample of copper chloride hydrate was calculated by measuring the mass before and after heating the sample. Afterwards, an oxidation-reduction reaction was conducted, resulting in elemental copper.
The reagents used were Diphenylamine reagent which contains concentrated H2SO4. The standard solution used for this test is the deoxyribose standard solution. In the sample, only a faint blue solution appeared, which indicates a small presence of deoxyribose. In test for Phosphate, the standard solution was the Phosphate solution and the reagents used were concentrated H2SO4, concentrated HNO3, 2.5% ammonium molybdate solution.
To identify the unknown acid. 4. To determine acid dissociation constant, Ka and pKa for the unknown acid. Introduction: Titration process is used in an acid-base experiment in order to determine the concentrations of solutions of acids and bases.
The internal standard method allows a very accurate analysis to be performed, since the behaviour of the species of interest is compared to that of a known substance which is present in a specified amount. It is usual to include an identical volume or mass of the internal standard into each prepared standard. This facilitates easier calculations of the composition of the
Purpose This experiment is to determine the concentration of the solute copper sulfate pentahydrate, and the unknown solution, by passing different wavelengths of light through each solution. Procedure Weigh out approximately 5g of copper sulfate pentahydrate. Record the mass and place the solute into a 50 mL volumetric flask. Fill half of the flask with distilled water, add the stopper for the flask, and lightly shake the flask, until the copper sulfate pentahydrate fully dissolved.
Back titration is used for this experiment as Calcium Carbonate is volatile and is an insoluble salt. Therefore they cannot fully dilute with water. As it’s chemical and physical properties are non ideal for a Direct titration, Direct titration cannot be used to determine the Calcium Carbonate content in toothpaste. In Back titration, excess volumes of HCl acids are added to allow for titration with NaOH. Eventually, the moles of CaCO3 can be found via stoichiometric property of the two reactants, and the mass of CaCO3 can be determined.