Tlc Lab Report

Procedure and Observations To begin the lab, first all the correct equipment and materials had to be collected

Using a properly setup Bunsen burner, remove the splint from the solution and immediately place in the flame and carefully look for any faint color. Be sure the splint itself is not burning, otherwise it may give a false positive. Certain colors are more intense and brilliant than others and, unfortunately, obscure those of less brilliance and intensity. Since a mixture of ions is not useful in identification, one must employ some method to remove interference. One technique is to use colored filters.

While waiting, label the four petri dishes B lactamase - pGlo, B lactamase/ ampicillin -pGlo, B lactamase / ampicillin +pGlo ,and B lactamase/arabinase/ ampicillin +pGlo. After labeling the petri dishes, remove -pGlo and +pglo test tube containers from the ice cup and place them on the heat rack (at 42℃). Only leave the test tubes on for 50 seconds. After

I. Purpose: To experimentally determine the mass and the mole content of a measured sample. II. Materials: The materials used in this experiment a 50-mL beaker, 12 samples, a balance and paper towels. III.

Lastly, product was GC traced to obtain the percentage of product and starting material within final product. Observations: Mixture turned yellow as sulfuric acid was added. During reflux condensation, sodium bromide dissolved and brown layer formed on top of solution as it boiled. Brown layer was alkyl bromide while clear, bottom layer was inorganic salts. After simple distillation, product was clear and colorless.

This enables a substance to be confirmed within a solution. With the intention of determining the unknown ion within the solution, the solution was placed inside of a test tube. It was significant to come across the correct order to precipitate the unknown ion within the solution by utilizing the solubility chart to determine a anion that can be used to precipitate the substance. It is important to note that certain solutions such as Na2SO4, Na2CO3, NaOH were used, in that order, to find out which ion is within the original solution. First, a few drops of Na2SO4 were added to the test tube consisting of the unknown substance.

In addition, Elution Volume (Ve) came out to be 5.00 ml. It was calculated by adding all of the fractions from beginning to the last fraction that contained yellow food dye. Yellow food dye was the smallest substance in size which made it easy for it to fit in all the pore sizes

Hence, ferrocene was the yellow layer and the acetyl ferrocene is the orange layer. Column chromatography can be separated from Part A through the same additions. The nonpolar compounds can be separated with addition of hexane. The polar compound such as diphenyl methanol would be added with ferrocene mixture. First, the benzophone would be separated in one flask.

After (NH4)2CO3 was added to the basic solution, heat is applied to the test tube. It was then centrifuged and the liquid was discarded into a waste beaker. The precipitate was then washed with deionized water and centrifuged once again. Acetic acid was then added to the solid precipitate to dissolve it, resulting in a clear solution. Potassium chromate (K2CrO4) was added in step 12 because the chromate (CrO42-) ion and the unknown cation.

While the solution dissolved, 50 mL of distilled water was added to a 150 mL beaker and heated on the hot plate. When the solution started to boil 2.65 grams of Na2SiO3*5H2O was added to the beaker with a stir bar and heated to a gentle boil. When both solutions began to boil, the sodium silicate solution was slowly added to the sodium aluminate. The solution was kept at 900C for 60 minutes and stirred with stir bar. After 60 minutes, the zeolite solution was cooled for 5 minutes and for the magnetized zeolite , 0.78 grams of FeCl3 and 0.39 grams of FeSO4*7H2O was added to the flask and stirred until the iron parts dissolved.

Materials and Methods: For the first part of the experiment is to measure the absorption of different diluted solutions of enzymatic extract. A spectrophotometer and three Erlenmeyer flasks are required. Set the spectrophotometer to 540nm. Number the flasks from one to three and add 30 milliliters of enzyme extract the flask marked one on it.

Since the solvent ran out of the column faster than the alumina settled, we kept adding more solvent so that there was always solvent above the alumina. If the column had ran dry it could’ve cracked, which would cause the bands to run and mix. When the alumina had settled and reached the marked line, about 8cm from the top of the column, we closed the column. We then added close to a centimetres worth of sand, using a spatula, which served to prevent the disruption of the well-packed stationary phase when we added more solvent throughout the chromatography process. We then opened the column until the level of solvent was on the sand layer, then we added five drops of the 1:1 Sudan Red 111 and Victoria Blue R mixture.

Set the wavelength to 470 nm, this is to measure the tetraguaiacol. Set the spectrophotometer to zero by using a blank. The blank should contain 13.3 mL of distilled water, 0.2 mL of guaiacol, and 1.5 mL of enzyme extract in a clean test tube. After, transfer a portion of this mixture into a cuvette, cover the top of the cuvette with Parafilm and then place the cuvette into the spectrophotometer and set it to

These color changes indicate a chemical change, which show that a reaction had occurred. In the first step when o-vanillin and p-toludine, imine was formed. The color change from green to orange suggests that imine appears as orange colored. In the second step, the addition of sodium borohydride reduced the imine into another derivative, which was yellowish lime color. The solution turned clear when acids and anhydrides was added, which indicated the precipitate were dissolved.

The solution was warmed to 65oC with constant stirring on the magnetic stirrer till the solid was completely dissolved. Stirring was stopped and 0.4g of charcoal was then added to the solution. After which the solution was transferred to a Buchner funnel with a moist filter paper and the clear filtrate was collected via suction filtration. The residue was washed with warm solution of 10.035g of KI and 15ml of deionized water. 200ml of water was then added to the filtrate in a 500ml beaker with constant stirring.