Chapter 4: Presentation, Analysis, And Interplantation Of
Chapter 4
PRESENTATION, ANALYSIS, AND INTERPRETATION OF DATA This chapter showed the data gathered by the researchers with the corresponding analysis and interpretation data. The presentation follows the sequence of the objectives of the study.
Objective1.To culture bacteria from the toilet bowl. Gram (-) bacteria
The cultured bacteria were obtained from the toilet bowl using the culture media; swabbing in the toilet was done and placed in a Fluid Thioglycollate Medium incubated for 24hrs at 37°C, after 24hrs the inoculums were inoculated in a Nutrient Agar for culturing bacteria incubated for 24hrs at 37°C, using MacFarland solution the cultured bacteria from the Nutrient agar were sub-cultured and inoculated to a Mueller Hinton Agar for
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In the 1st trial, it showed a zone of inhibition of 13mm, 2nd trial showed a zone of inhibition of 12mm and 3rd trial showed 18mm. This implicates that the garlic extract showed a zone of inhibition in the three different trials that are a positive result for its antimicrobial activity, and its effectivity against the cultured bacteria that were present in the toilet bowl.
The three trials showed three different results because of the factor that each plate of Petri dish produced a different amount of bacteria present. The plate of the second trial showed numerous amounts of bacteria that are thicker than the plates of the first trial and the third trial. This implicates that the pure garlic extract showed antimicrobial activity and the result differs from the bacteria present.
Objective3. Determine the antimicrobial activity of garlic extract using different concentrations.
Table3. Zone of inhibition of formulated garlic extract
Key: (-) no zone of inhibition, (mm) millimeter
Concentration Zone of inhibition (mm) Trial 1 Trial 2 Trial
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The significant analysis connotes that the results of the experimental study of the formulated antimicrobial spray preparation using garlic extract which gives a positive result, showed a zone of inhibition against the cultured microbes but not that effective compared to the positive control which showed a more wider zone of inhibition compared to the three different concentrations
Terrance V Corder
1516 Words | 7 PagesAfter lawn inoculating a Meuller Hinton plate and placing the samples of medication, the plate was then incubated for one week at 37 degrees Celsius. The first medication choice was Trimethoprim, this produced a zone of inhibition of 16mm, therefore being sensitive to the bacteria. Antibiotic number two was nalidixic acid, this too, has a zone of inhibition of 16mm but is considered intermediate. The next antibiotic was erythromycin which produced a zone of inhibition of zero and was therefore resistant. The last antibiotic that was chosen to be used in the experiment was ciprofloxacin.
Unknown Culture Lab Report
1054 Words | 5 PagesIdentification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
Unknown Bacteria Lab Report
1200 Words | 5 PagesThe purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
Lab Report Identifying Unknown Microorganisms
1596 Words | 7 PagesI expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided
Unknown Bacteria Lab Report
1034 Words | 5 PagesTherefore, acid fat, lactose fermentation, mannitol fermentation were not needed to be performed, because they are selective to a specific thing. As a result, Unknown bacteria “W” was concluded to be gram stain positive, endospore positive, bacilli shape (rod shape), and arranged in chains (strepto-). Test Purpose Reagents Observation Results Gram Stain To determine gram reaction of bacteria.
Unknown Bacteria
557 Words | 3 PagesIn the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Unknown Lab Report Essay
408 Words | 2 PagesThe unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
Unknown # 10 Essay
650 Words | 3 PagesBy Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic
Alcaligenes Faecalis Experiment
636 Words | 3 PagesAccording to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC). We consider we have a match with the species Alcaligenes Faecalis because of the following reasons: Fermentation tests performed (Durham sugars) were negative, which indicate that our bacteria use a different metabolic means for growth (non-fermentative gram-negative bacteria).
Dog Saliva Hypothesis
3687 Words | 15 PagesHypothesis: It is hypothesised that dog saliva will be effective in killing bacteria. Aims: To prove that dog saliva has the ability to kill bacteria. To find possible uses for dog saliva as a disinfectant. To compare the effectiveness of dog saliva as a disinfectant to commercial
Exercise 14 Unknown Identification Lab Report
449 Words | 2 PagesThe second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate Staphylococcus aureus, Streptococcus lactis and Bacillus subtilis.
Blood Agar Lab Report
1057 Words | 5 PagesAfter a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.
Kirby Bauer Diffusion Test Lab Report
624 Words | 3 PagesAseptic technique was initiated at the beginning of this experiment by cleaning the work surface with disinfected wipes. Personal protectives equipment was also worn. The material utilized in this experiment was: S. epidermidis culture broth, sterile cotton swab, streak plate, forceps in 70% alcohol, a lit tea light, and the three antibiotic disks (novobiocin, gentamicin, penicillin). The first step, I divided a plate into three quadrants and labelled them with the different antibiotic names. Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture.
Staphylococcus Epidermidis Research Paper
452 Words | 2 PagesAfter experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish
Biochemical Test Lab Report
1371 Words | 6 PagesBiochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
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