After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.
Aseptic technique was initiated at the beginning of this experiment by cleaning the work surface with disinfected wipes. Personal protectives equipment was also worn. The material utilized in this experiment was: S. epidermidis culture broth, sterile cotton swab, streak plate, forceps in 70% alcohol, a lit tea light, and the three antibiotic disks (novobiocin, gentamicin, penicillin). The first step, I divided a plate into three quadrants and labelled them with the different antibiotic names. Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture.
In this experiment, Tetracycline and Ampicillin were used to test for the antibiotic resistance of Pseudomonas fluorescens, lab-grown bacteria. The bacterial colonies showed resistance to Ampicillin; however they did not show resistance to Tetracycline. The results suggested that Tetracycline was much more effective in killing the bacterial growth, in comparison to Ampicillin. It was confirmed that a single dose of Tetracycline had killed the surrounding bacterial growth, as the bacterial colonies around where the drug had been placed, died and showed no growth. The bacterial plates also suggested that Ampicillin was ineffective in killing the bacteria, as zones of inhibition were not present, despite using two disks of this drug.
Determined/chose/selected three hand washing regimen(s) – see Table # 1 2. Washed each finger with the given regimen 3. Placed the washed samples on the corresponding quadrant of the agar plate near the center 4. Allowed the cultures to incubate at body 37 degrees C temperature for 7 days 5. Observations were made of each culture with the use of a dissection microscope to characterize the microorganism/colonies present in the agar plate.
3.) MRSA is resistant to beta-lactam antibiotics (penicillin, methicillin, etc.) and can be more virulent and invasive than Staphylococcus Aureus. MRSA carries the mecA gene, which allows the bacterium to fully construct a cell wall by coding for the penicillin binding
The first of the four test was a Gram-stain. Once the experiment was completed the slide was then placed under a microscope where it was then categorized as Gram-negative bacilli. Its pink color when viewed through the microscope indicated that the test was Gram-negative and its rod shape indicated the morphology was bacilli. With these results the next test to be conducted was the citrate utilization test. A citrate tube was inoculated with P. aeruginosa and incubated at 37 degrees Celsius at 24 hours.
For instance, Rifampin, an antibiotic used to treat Tuberculosis, Neisseria meningitidis- also known as Meningitis- or infections in the nose and throat. Another treatment is a vaccine known as Bacillus Calmette- Guerin to prevent T.b. or to heal bladder tumors or bladder cancers. “It wasn’t that all these infectious diseases had simultaneously changed to be less deadly (Humphries/ Bystrianyk 15). It was the environment in which they had existed had shifted from one where they were very often deadly to one where they were they were not a significant threat (Humphries/ Bystrianyk 15).
For the orange, the distance the band traveled was 39mm and the distance solvent traveled was 39mm and the Rf was calculated at 1mm. For strawberry the distance band traveled 38mm and the distance the solvent traveled was 41mm and the calculated Rf was .927mm. The colors for strawberry, orange and grape Kool-Aid are made with food dye. There is also salt that is found in Kool-Aid that is why when placed in the tubes containing %NaCl, the solvent caused the drops on the strips to travel so far. The results in this were expected.
Cancer prevention Fennel is loaded with its own share of antioxidants and phytonutrients. Studies show that the Fennel seed extract can inhibit growth of tumor. It’s probably because of its flavonoid and alkaloid concentration. These chemicals present in fennel can fight the free radicals roaming in our blood, which are supposedly responsible cancer. 5.
The effect of the ammonia and ammonium was determined by the pH levels, as the pH level lent towards a stronger acid, the duckweed experienced less growth. What also affected the growth was the combination of the ammonium and ammonia at different concentrations. The conclusion that I made of this study was that ammonia and ammonium are duckweeds best source of nitrogen which they need for survival, but in high enough concentrations and with a strong enough acid, the effects the NH3 and NH4 had on the duckweed could be damaging towards its growth. All of the people who were involved with this study have their doctorates in water research, meaning that the results obtained will be accurate and reliable, making this a valid