Plant Culture Case Study

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Materials and Methods 2. Materials and Methods 2.1. Plant culture Tomato seeds (Lycopersicon esculentum var. Five Star F-1 Hybrid) were gotten from the local market. Before sowing, the seeds were surface sterilized with a mixed solution containing 49% sterile double-distilled water (DDW), 50% ethanol, and 1% sodium hypochlorite for 5 minutes, then vigorously rinsed with sterile DDW. The tomato seeds were germinated on 2 sheets of sterilized filter paper in a Petri dish (12-cm diameter). The Petri dishes were kept in the dark for germination. 2.2. SP and NO treatments under NaCl stress After two weeks of germination treatments were applied to each Petri Dish. The treatments were given as follows: (i) 0 μM NO + 0 nM SP, (control), (ii) 100 μM NO, (iii) 200 μM SP, (iv) 100 μM NO + 200 μM SP, (v) 100 mM NaCl, (vi) 100 μM N0 + 200 μM SP + 100 mM NaCl, and (vii) 200 μM cPTIO + 100 μM NO + 200 μM SP + 100 mM NaCl. Analytical grade chemicals were used in this study. Sodium nitroprusside [SNP: (Na2 [Fe(CN)5NO].2H2¬O] was used as the NO donor, while cPTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide] was used as the NO scavenger. NaCl treatment was applied to the Petri dish having tomato seedlings in order to attain the final concentration. The Petri dishes were arranged in a sample-randomized …show more content…

(1995). Leaves were washed 3 times with DDW to remove surface contamination, and leaf discs were cut from young leaves and placed in sealed vials containing 10 mL of DDW, followed by incubation on a rotary shaker for 24 hours, after which the electrical conductivity of the solution (EC1) was determined. Then, the samples were autoclaved at 120 ΟC for 20 minutes, and the electrical conductivity was measured again (EC2) after the solution was cooled down to room temperature. The electrolyte leakage was defined as EC1/EC2×100 and expressed as

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