pneumoniae and E. aerogenes are both gram negative bacteria associated with many health related problems worldwide. K. pneumoniae is a common hospital-acquired pathogen, causing nosocomial pneumonia, urinary tract infections, and intra-abdominal infections29,30. E. aerogenes has emerged as an important hospital pathogen since 199231,32. It is the third leading cause of respiratory tract infections by gram-negative bacteria, after Staphylococcus aureus and Pseudomonas aeruginosa33. Antibiotics are commonly used against these bacteria but the major concern is the increasing antibiotics resistance in these bacteria.
Pet. Ether fraction showed the most potent antimicrobial activity in different dilutions with time interval of 4 hrs is listed in Figure 2 and 3. The sub fraction D had shown highest anti P. acnes activity with zone of inhibition 26.0 and 32.0 mm at concentrations 1 mg/ml and10 mg/ml respectively are listed in Table 2. The fraction D was further fractionated with the help of sub-column. Six different fractions were collected out and evaluated for antibacterial activity.