Transgenic Plant Research Paper

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Researchers are actively making progress in introducing new plant trait by using recombinant DNA technology and one of it is through transgenic technology. Genetic engineering has the potential to fasten the process of plant genetic manipulation and also expand the scope of the field research other than for agriculture purposes. Nowadays, transgenic plant is widely applied in agriculture area to increase the production of food as well as improving the food quality either in its nutritional or physical aspect (Hoque, Mansfield & Bennett, 2005).
Kumar et al. (2005) stated that, the production of transgenic plant generally was done by artificially inserting new gene of interest to the plant sequence instead of gene used in plant
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The Agrobacterium method is mainly used for dicots species to produce transgenic tomatoes and soybeans meanwhile, the gene gun technique is mainly applied for the transformation of monocot plant species such as corn and rice (Hiei & Komari, 2008). However, according to Davis et al. (1999), the first transgenic rice plant was produced through micro projectile bombardment in the late 1980s at which the gene transfer was mediated by electroporation. The Agrobacterium method using Agrobacterium tumefaciens became prevalent in producing transgenic plant in mid of 1990s due to its high efficiency of plant transformation compare to other method (Rivera et al., 2012). Rivera et al. (2012) explained that this high plant transformation efficiency is due to large fragments of DNA transferred into the chromosome and it is also due to low transfer DNA (t-DNA) integration into the…show more content…
In order to produce the transgenic rice plant, the rice callus from Nipponbare variety was transformed using miRNA construct against lipases enzyme. The gene of the precursor miRNA was artificially inserted into the Ti plasmid which occurs naturally in the A. tumefaciens. The method used for the production of transgenic rice plant in this practical is the Agrobacterium method in which the t-DNA was transferred once the Agrobacterium infects the plant cells. Thus, transformed cells can be produced once the t-DNA integrate with the genome of the plant and further develop into a GM rice plant.
Materials and methods
The rice line used for the production of rice callus with miRNA construct against lipase gene using Agrobacterium tumefaciens is from the Nipponbare variety.

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