Gene Transfer Lab Report

Introduction: Transforming a gene or genetic information from one organism into another with the hopes that if done successfully the organism with the new DNA will be given new traits is a method known as genetic transformation (Rafter). Genetic transformation is used quite frequently in today’s world, form medicine to agriculture.

Bethany Brookshire, the author of the article “New gene resists our last-ditch drug” found in the Society for Science & the Public, invoked fear and urgency in teen readers fascinated with biology and health. Throughout her article, Brookshire establishes that doctors, farmers, and everyday citizens should be cautious in the use of antibiotics and use methods to limit the spread of harmful bacteria worldwide. She gains her readers’ attention and trust by quoting information from several scientists in different fields and from different parts of the world. Although her syntax was rigid and overly simplified, Brookshire connect to the teen readers ******

The purpose of this experiment was to insert the plasmid glow green into the bacteria with a gene of interest to produce the protein that make the bacteria glow green along with the presence of arabinose and the presence of ampicillin. Many scientists are experimenting different kind of genes that can inserted into the organism for survival. The technique of transformation was used in this experiment to give the organism a new trait that they did not possess in their life. In this experiment, the bacteria were added to four plates with certain conditions such as the existence of plasmid, ampicillin, and arabinose to see whether the bacteria grow and glow green. The results showed that the LB/amp/araC +pGLO produce a lot of colony and most

Transformation in bacteria usually takes place when a bacterial cell accepts strange DNA and integrates to its own DNA. The transformation normally takes place within plasmids, which are tiny circular DNA molecules that have been separate from its own chromosome. The copies of the same plasmid range from 10 to 200 copies within a cell. These copies of plasmids may multiply when the chromosome replicate or multiply independently. One plasmid has a range of 1,000 to 200,000 base pairs. R plasmids are responsible for carrying the gene for resistance to antibiotics e.g. ampicillin, which are normally used in the lab. The normal function of a plasmid is to transport genetic information essential to the survival of the bacteria. (Barnnet, 1995). The plasmid can work as vectors for introducing strange DNA. Restriction enzymes are normally used cut foreign DNA and placed it into the plasmid vectors. This lab used Escherichia coli (E. coli) bacteria (Kok , 19840). This is because Escherichia coli can be simply grown in Luria broth or on agar, and also has a comparatively small genome of five million base pairs.

n this lab, there were four objectives needed to be met. The first one was to perform a genetic transformation procedure, the second was to move genes from one organism to another using a plasmid as a vector, and the third was to manipulate tools of biotechnology. The bacteria E. coli was used to manipulate and transform. The E. coli would be tested for ampicillin resistance and a green fluorescent glow. One hypothesis made for this lab was that the bacteria that developed a resistance to ampicillin would reproduce even in the presence of the ampicillin. Another hypothesis made was that the bacteria would glow with the addition of the sugar arabinose.

In this lab, the goal was to transform bacteria with genes that included fluorescence as well as antibiotic resistance that were taken from a jellyfish. Transformation is transferring a gene from one organism to another. Certain precautions had to be made before doing this lab since every step had to be done very quickly to prevent too much contamination. The first step in starting the transformation is to add the transformation solution into the +pGLO and -pGLO test tubes. After this is done, you put both tubes in ice and then put bacteria in both tubes. Then, put the pGLO plasmid in the +pGLO test tube but not the -pGLO. Place both of the tubes back in the ice. While the tubes are in the ice, label 4 agar plates: +pGLO LB/amp, +pGLO LB/amp/ara,

To begin, during this lab experiment, genetic transformation was successfully carried out. After observing the agar plates, it was found that only the plate with ampicillin and no pGLO plasmid did not grow any of the E.coli bacteria. All three of the other plates grew the E.coli bacteria, however it grew differently in each plate. In the control plate where the pGLO plasmid, ampicillin, and arabinose were not present, the bacteria grew in the pattern that it was spread in originally. In the two other plates, bacteria grew in colonies that eventually joined together due to prolonged time in the incubator. Although transformation occurred in both plates with the pGLO plasmid, only the plate containing arabinose sugar had fluorescent colonies when observed under UV light.

With the future of medical science right in our grasp, false claims could be interpreted by the general public leading to a confusion on who is truly right. With this article published on USA Today, the amount of bias and false facts that are able to slip through the cracks for the world to see is astounding. With this article, two medical professionals that are untrained in the study of stem cells makes false claims regarding alternatives to embryonic stem cells that could lead to public misrepresentation of the facts on fetal stem cells.

Farmers and ranchers have been manipulating genes for plants and animals well before gene sequencing and molecular techniques were practiced. The size of a grapefruit and coloring on cattle may be attributed to the former. With new knowledge about animal and plant genomes, scientists can now delicately screen, edit, and splice genes for varying reasons. This paper intends to explore some of the more common techniques in genetic engineering (prenatal screening, gene manipulation, and cloning) for humans and the ethical issues surrounding them.

Embryonic stem cell research is a process in which scientists isolate material from an embryo that was conceived five to seven days prior. At that point embryos are called blastocysts. The outer layer of the blastocyst will become the placenta while the inner cell mass will become a fetus. It is from the inner cell mass that scientists isolate the stem cells. Even though this research might become useful in the medical field, scientists should stop embryonic research because it is not productive, and there are better ways to get the desired results. Also, it is not moral to use and destroy embryos in this way.

Gibson et al., carried out this experiment to synthetically generate a DNA genome. According to this presented paper there are inadequate knowledge about the functionality of genome on different parts of the cell. Synthesizing a genome of a simple bacteria such as M. mycoides Capri and monitoring the regular functionality of this genome in a recipient bacteria such as M. capricolum is very important. In this manner scientists can learn more about the correlation of each genome and their function in a cell. This experiment proved that the synthesized gene could maintain the functionality of M. capricolum bacteria, and this bacteria was able to divide and have progeny similar to the donor bacteria M. mycoides (Gibson et al.).

Hello everyone and good afternoon, I am professor Villasana and I come here today from Stanford University. This evening I will be explaining to you all what Friedreich's Ataxia is and how it is that this condition comes about in the human body. I plan on simplifying the explanation of this complex genetic condition and educating you all a bit more of this disease to also spread awareness.

Embryonic Stem Cell are obtained from embryos into distinctive stages before the time that implantation would normally occur in the woman’s organ in the

Regenerative medicine has a treatment nowadays for our cells, organs and tissues repair and replacement normal function. Hence demands increase in population for organ transplantation. Research has conducted for recent and alternative therapies. Regenerative Medicine can medicate few cells that were damage due to agedness and congenital defects. In addition stem cell has a regenerative medicine; it regenerates, repairs, and restores functionality. Regenerative medicine has a cure to failing or damage tissues. Stem cell increase years to human life, cure disorder and it also includes make one seems juvenile. As a result this gives leisure to the opening of stem cell clinics to the public in the Philippines. Cellular Therapeutics Center of Makati

Do you know that based on the scientific studies, 90% human cloning tuned out to be unsuccessful. Human cloning(modifying babies) is a process of producing new identical babies by altering their genomes. Some of studies show that scientists successfully cloned animals such as cows, Pigs, and sheep. For the past 3-5 years human cloning have a lot of debates and controversies between peoples. However Human cloning is dangerous for the new engineered baby and their moms, so it should be banned to prevent new disease, to constantly limit the population of dying human beings, and to stop unnecessary fees to modify babies.