Tretinoin Lab Report

5.1 MATERIALS USED
Tretinoin (drug) was obtained as a gift sample from M/s Psycorem Pharmaceutical Pvt. Ltd Ludhiana (India).
Tristearin, Pluronic F68, Carbopol 940P, Phospholipon 90G were purchased from M/s Himedia, Mumbai, India. Cholesterol, Oleic acid, Triethanolamine (TEA), Acetone and Ethanol were purchased from M/s Central Drug house Pvt. Ltd. New Delhi. India. Double distilled water was used throughout the study.

5.2 METHOD FOR PREPARATION OF TRETINOIN LOADED NLCs
NLCs were prepared by Melt emulsification and low temperature solidification method Tretinoin and phospholipon 90G were dissolved in methanol and mixed with an acetone solution containing a blend of Tristearin and Oleic acid. The mixture was sonicated for 15 minutes and added drop wise to Pluronic F68 solution. After that, the mixture was stirred at 3000 rpm for 30 minutes at 70oC. The mixed solution was transferred to an ice water bath and stirred for 4 hours at 3000 rpm. The NLC dispersions were lyophilized.

5.3 OPTIMIZATION OF DRUG LOADED NLCs
Preparation of Tretinoin loaded NLCs involves various process

Dialysis membrane having a pore size 2.4 nm and molecular weight cut off between 12,000 and 14,000 was used. The membrane was soaked in double distilled water for 12 hr. before mounting in a Franz diffusion cell. About 1ml of semisolid preparation of NLC was applied to the donor compartment, and the receptor compartment was filled with phosphate buffer, pH 7.4 (14 ml). During the experiment, the solution in the receptor side was maintained at 370C and stirred at 800 rpm with Teflon coated magnetic stirrer bar. At fixed time intervals, 3ml of the sample was withdrawn from the receiver compartment through side tube and analyzed spectrophotometrically at 353 nm by the UV-VIS spectrophotometer. A graph of % cumulative drug release against time was plotted as depicted in Table 5.6 and shown in Fig 5.5