Fresh And Dry Fruit Lab Report

RESULTS

Phytochemical analysis of fresh and dry fruits extracts of Tribulus terrestris

Components Aqueous Exract Methanolic Extract Ethanolic Extract Fresh Fruit Dry Fruit Fresh Fruit Dry Fruit Fresh Fruit Dry Fruit

Tannins + + + + + +

Flavonoids + + - - - -

Saponins + + - - - -

Proteins - - + + + +

Steroids + - + - + -

Alkaloids + + + + + +

Carbohydrates _ _ + _ + _

Minimum inhibitory concentration of fresh and dry fruit extracts of Tribulus terrestris

S.NO

Test Microorganisms

Control

Ampicillin/Amphoter-icin B MIC of Aqueous extract mg/ml MIC of Methanolic extract mg/ml MIC of Ethanolic extract mg/ml Fresh Fruit Dry Fruit Fresh Fruit Dry Fruit Fresh Fruit Dry Fruit

Gram positive organisms

1 S. aureus 0.12 2.49 2.35 0.63 0.51 0.72 0.54

2

subtilis 0.06 1.25 1.23 0.23 0.15 0.23 0.19

3 B. cereus 0.08 1.25 1.23 0.25 0.21 0.25 0.21

Gram negative organisms

4 E. coli 0.19 1.25 1.23 0.58 0.48 0.58 0.52

5 K. pneumoniae 1.35 2.50 2.49 1.35 1.19 1.35 1.28

6 P. aeruginosa 1.40 >4 >4 1.40 1.28 1.35 1.28

Fungi

7 C. albicans 0.05 >5 >5 0.09 0.06 0.13 0.06

8 A. flavus 0.07 3.24 3.22 0.09 0.08 0.10 0.08

9 A. niger 0.03 1.25 1.20 0.08 0.07 0.10 0.07

Antibacterial activity of aqueous extract of Tribulus terrestris fruit by disc diffusion

Test Microorganisms Inhibition Zone in (mm) Aqueous Extract Concentration(µg/ml) Streptomycin

(+) Control DMSO

(-)Control

1000 750 500 Fresh Fruit Dry Fruit Fresh Fruit Dry Fruit Fresh Fruit Dry Fruit

S. aureus 4mm 3mm 3mm 4mm 2mm 3mm 5mm -

B. subtilis 6mm 4mm 5mm 3mm 4mm 2mm 7mm -

B. cereus 5mm 4mm 4mm 2mm 3mm 1mm 8mm -

S. aureus 5mm 2mm 4mm 4mm 5mm 4mm 9mm -

E. coli 6mm 5mm 4mm 3mm 2mm 1mm 8mm -

K. pneumoniae 7mm 5mm 7mm 6mm 5mm 3mm 10mm -

P. aeruginosa 4mm 3mm 3mm 2mm 2mm 1mm 6mm -

Antibacterial activity of methanolic extract of Tribulus terrestris fruit by disc diffusion

Test Microorganisms Inhibition Zone in