The built-in probe in the chamber measured the dissolved oxygen concentration in the chamber’s water (mg/L), which could reflect the oxygen consumption rate of goldfish. After the fish accommodated to the new environment, the oxygen concentration data were collected every 20 seconds for 10 minutes by the Logger Lite program. The data were then plotted into a scatter chart and analyzed with a linear trendline, to obtain
This experiment aims to separate the components of the green colored food dye and get the TLC profile of each eluent collected. III. Experimental Procedure Before starting with the column chromatography for food dye, the right solvent must be chosen between 2-butanol with acetic acid, ammonia in butanol, 1 part 1-butanol 1 part acetic acid, and 2 parts methanol 1 part water. In choosing the appropriate solvent for column chromatography, the solvent system must give a TLC profile wherein most of the spots are well separated and has a Rf value within 0.3-0.5. For TLC profiling, 4 TLC plates were prepared for the testing of each solvent.
The repression system targets the intracellular trp concentration whereas the attenuation responds to the concentration of charged tRNA. Thus, the trpR repressor decreases gene expression by altering the initiation of transcription, while attenuation does so by altering the process of transcription that's already in progress. While the Trp R repressor decreases transcription by a factor of 70, attenuation can further decreases it by a factor of 10, thus allowing accumulated repression of about 700 fold. Attenuation is made possible by the fact that in prokaryotes, the ribosomes
Processing Raw Data Calculations The equationsRf(A) =LAL0 and Rf(A) =LBL0 were used to calculate the Rf values, A standing for the distance the green pigment traveled and B standing for the distance the yellow pigment traveled. For trial 1 of Nandina domestica, the L0 = 3.50 cm and the LA = 0.70 cm. Therefore, Rf(A) =LAL0= 0.70 cm3.50 cm= 0.200. A Rf value of 0.200 corresponds with the amino acid arginine. This was repeated for each trial of each leaf and the amino acids were identified.
Crops and farming would be at a drastic low because what kind a food would be able withstand that kind of heat? Bill McKibben also talks about 565 Gigatons of carbon. No more than that is the whole planet able to emit, or the two degrees would become more and more
Method A more detailed method is provided in the 280.201 Industrial Microbiology lab manual. However, the basic steps of carrying out this experiment are as follows: • Five agar plates are provided including: 1. Nutrient agar 2. Mannitol Salt agar 3. Eosin-methylene blue agar 4.
The American has average income spend less than $8 per person per day for their meals and beverages. Meanwhile, poor people just spend only $25 per person per week. This is a big gap in their cost of meal. The main cause is healthful diets cost more than unhealthful diets (Drewnowski & Darmon, 2005). Besides, America is the unhealthiest countries in the world.
2. You have been asked to set up a dilution series, and then use spread plates to determine the viable cell count. Why is it necessary to use a dilution series when isolating bacteria from a biological sample using spread plating? [5 MARKS] It is vital to use a dilution series to reduce the concentration of the original biological sample so it is easier to count the number of isolated colonies which are present on the spread plate. The diluted samples will have a workable number of colonies on the spread plate which can then be used to calculate the number of bacteria which were present in the original undiluted sample.
Hernandez 1 Hector Hernandez ENGL V01A February 6, 2018 The World of Plenty Our vast population growth is asphyxiating our world. Over the past century alone the population has gone from 1.5 billion to over 7 billion. We as human beings have evolved to the point to where a once fatal broken leg is means little to us. Advances in technology have helped us survive for longer periods of time, which is a blessing and a curse. Many scientists believe the maximum carrying capacity of earth is around 10 billion, and this number is based on the number of resources available to us and the limited availability of fresh water (Wolchover).
It also shows that as the distance from the plant increases, that the rate of photosynthesis decreases causing the number of bubbles from the cabomba plant to go down. The closer the light source was to the plant the more oxygen was released from the plant by using a progress called Photosynthesis. The average number of oxygen bubbles created in one minute was 151 where the light source was 20 centimetre away. As my results in my table show the further the light source is away from the cabomba weed the slower it gets to create oxygen. My furthest distance I measured was 40 centimetres and the average bubbles realest was 103 per minute.
If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein. If there is a color change, then it is known that protein is present in the solution.
The same process was repeated but the net was at the 10-meter mark and the students started shuffling at the five-meter mark. In the capture-mark-recapture method, the crayfish were marked using blue and pink nail polish allowing for the crayfish to be dumped back into the sampling area. The removal method group kept all of the crayfish collected aside to ensure only the new crayfish were collected. The 10-meter portion of the stream was then collected four more times, giving a total of five separate collections for each
A Paleo diet will be mostly composed of high-protein dishes. Research signifies that whenever the common person feeds on a high-protein diet regime for half a year, they may lose somewhere between 10-15 pounds. That would be without the need of increasing physical exercise. You can lose a lot more weight when you enhanced your physical exercise, making a Paleo diet an ideal choice for everyone who would like to shed weight or who merely would like to turn out to be total
RDI values suggest that around 1000mg of calcium should be consumed per day. This diet provides an average of 371.53mg per day, less than 50% of the recommended amount [refer to Graph 1], meaning the intake of calcium is significantly lacking. Insufficient intake of calcium could lead to the reabsorption of skeletal calcium, leading to osteoporosis in the long term (MelinePlus, 2015). Sodium is required in the diet to assist in the contraction and relaxation of muscle, and work with potassium to control fluids entering and leaving cells. RDI values suggest that 460-920mg should be consumed per day.
The beginning reaction that occurred at the pH level of 1 shows that the mean reaction rate was incredibly low, at 2 mL/minute. This then increased by 57 units once it reached its peak productivity of 59 mL/minute observed at pH 8. pH levels 6, 7, and 8 only varied between 1 and 2 mL/minute, which demonstrated similar rates of reaction. At pH 10, the reaction rate decreased considerably as it declined by 58 mL/minute, and maintained that productivity at pH 12. The scatter graph included in the results section further solidify and visually represent these observations. The reaction rate of the catalase exposed to pH 1 is barely conceivable on the diagram as its average rate of reaction was 2 mL/minute.