Trypanosomiasis Lab Report

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Trypanosomiasis is caused by a parasitic protozoan of the family Trypanosomatidae and genus Trypanosoma. Trypanosomes can infect mammals including humans and can exhibit very high parasitaemia as blood parasites; it is also considered a tissue parasite, due to its ability to invade the nervous system. It is the agent that causes sleeping sickness in humans.
This disease in vertebrates is colloquially known as Surra (Derived from Marathi) or Mal de caderas in Brazil but this term was also at times used for rabies. The spread observed in Bolivia around 1840. This disease is highly fatal, unless treated, and occurs in most vertebrates including the Equine family where the parasite principally is transmitted by the bite of the horse-flies
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Preparation of 1.8% agarose gel:

1.8 grams of agarose powder was weighed and transferred into a microwaveable conical flask.

100ml of 1X TAE buffer was added to the flask.

The solution was mixed well and warmed in a microwave oven for 1-3 minutes until the agarose was completely dissolved until a clear homogenous solution was obtained. The heating was done with small intervals for occasional swirling to ensure minimum loss of the buffer due to evaportation.

Use of mittens is recommended to avoid burns. Care must be taken not over boil the mixture.

The solution was left to cool and on reaching a temperature of around 50℃ (The flask feels bearable to human touch)

8 µl of Ethidium bromide (EtBr) was added to the flask and swirled for uniform mixing

EtBr binds to the DNA molecule by intramolecular interaction (secondary structures) of RNA molecules and through van der Waal's interactions between the bases thus and allows visualization of the DNA under ultraviolet (UV) light.
EtBr is known to have carcinogenic effects, proper protection like gloves is

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