The principle involved in this test is the precipitation of phosphate which bores a yellow-colored solution and yellow precipitate. In the sample, neither a yellow-colored solution nor a yellow precipitate appeared which indicates the absence of phosphate in the sample. In the test for Purines, or Murexide test, the standard solution used was solid guanine. The reagents used were concentrated HNO3 and 10% KOH. Positive results should be red-purple residue.
Therefore, if no sugars are present in the whipping cream, there cannot be any starch as well. Moreover, it was confirmed that soy milk does indeed contain starch. This was also hypothesized as it was assumed that since soybeans are a legume, they would contain starch. The experiment confirmed this result as when the iodine solution was added, the milk turned a dark grey colour at the top of the test tube and when it was mixed, the entire solution became a light grey colour. A blue or black tinted colour is an indicator of starch in a product, so the grey hue that the soybean milk possessed confirmed the hypothesis.
The test used was Enzyme-linked immunosorbent assay (ELISA) which is a test that uses antibodies to see a change in color to identify an antigen. Fresh venous blood samples were drawn into pyogen-free blood collection tubes without additives, immersed in ice, and allowed to clot before centrifugation. All serum samples were stored at -70°C. Serum PAF-AH levels were measured with ELISA kits. The detection limit of this assay was 0.074 ng/ml.2 The results for this study were that Serum PAF-AH levels were significantly higher in SSc patients (130.4± 69.5 ng/ml) than in healthy individuals (81.6 ± 34.8 ng/ml; P < 0.05 ;).
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate. All enzymes are under the class of protein biomolecule. Amino acids are the basic units that are combined to make up an enzyme.
Since lead was not present, the "Part A" test tube, which contained precipitate from the "Unknown 4" substance, was now to be tested for the presence of silver. The "Part A" test tube 's precipitate was first washed with deionized water to remove any contaminants. When 2 mL of 6M NH4OH was added, white precipitate deposited at the bottom of the test tube. After centrifuging the "Part A" test tube, the liquid was poured into a clean
To check the anti-aggregation property of Thymoquinone on diverse proteins (2). Project summary There are several evidences that Thymoquinone is a potent inhibitor of amyloid beta peptide (Alhebshi AH, 2013) but there is no evidence that it can also be a potent inhibitor of diverse class of standard proteins. So, we will check the effect of this compound on the aggregation behavior of standard proteins such as Bovine Serum Albumin, Human Serum Albumin and other mammalian serum albumins proteins. Till now we know that these standard proteins and some amyloidogenic proteins such as amyloid beta peptide, tau protein, IAPP, TRR protein are responsible for the diverse diseases which are caused by the aggregation of proteins and amyloid formation
Quantification and trypsin digestion of polypeptides Protein concentration was estimated by Bradford assay, and 100µg of total protein from each sample was subjected to in-solution trypsin digestion to generate peptides. Initially, treating the sample with 5µl of 100mM dithiothreitol in 50 mM ammonium bicarbonate for 30 min at 60ºC and alkylation with 200mM iodoacetamide in 50 mM ammonium bicarbonate at room temperature for 30 minutes reduced the protein disulphide bonds. Proteins were then digested with 4µg of sequencing grade-modified trypsin (Sigma) in 50 mM ammonium bicarbonate by incubating overnight at 37ºC. The trypsin digestion reaction was stopped by 1µl of 100% formic acid. The digested peptide solutions were centrifuged at 14000
1) Present the gel electrophoresis image of plasmid digestion experiment with the correct label. Describe the results of the experiment and list down possible problem that you observe based on the electrophoresis image. Plasmid P1-1 and P1-2 undergo single digestion. Plasmid P1-3 undergoes double digestion. Single digestion is only one restriction enzyme which has been used to digest a DNA.
In the first stage in the normal body, vitamin B12 binding proteins that found in saliva to enters the stomach. Then the stomach uses hydrochloric acid to liberate vitamin B12 from the proteins. However, in the first stage of pernicious anemia, the stomach excretes a small amount of hydrochloric acid. This leads to a failure of separate vitamin B12 from food proteins, which inhibit vitamin B12 from absorption. In addition, the lack of secretion of hydrochloric acid provides a suitable environment for a reproduction of gut bacteria.
We place a layer of stain in the bottom of a glass coplin jar (about 3 mL), then add Buffer to a level that will just cover the slides (except for frosted ends!) when they are in the jar. A little practice will tell the amount of buffer to add. Place the slides, Back-to-back into the slots of the jar, and stain at room temperature for about 50 Minutes. 3.
In this lab we used mechanical weathering to shake up sugar cubes inside of a mason jar. To complete the lab we needed to shake for 3 minutes to see how the mechanical weathering actually works. The sugar cubes represented the rocks and minerals. We used sugar cubes because they have a mass less than other rocks and minerals. I thought as we shook the cube it would just crumble into little bits.
My group’s hypothesis was that the sugar cubes would break up. The mass of one sugar cube was originally 2.35 grams. After it was shaken by all three of us for three minutes, it’s mass was .96 grams. Our data cannot be used for everyone, however. How rapidly, strong, and fast you shake can make a difference.
No quick endospore stain was performed to validate this assumption since only one assigned organism was endospore forming and unlike Unknown #10, that organism was Gram positive. By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic
Our last petri dish was minus DNA and lysogeny broth, in this petri dish we saw 7 colonies of bacteria and there were a light yellow color. These results mean that we didn’t infect that petri dish with ampicillin from the pipette. Our expectations were both unsupported and confirmed. For example, our minus DNA and lysogeny broth had bacteria growth while our LB/amp/ara plus DNA dish had no growth and did not glow. Some problems that might have occurred in the lab is that we either put too much ampicillin in our LB/amp/ara dish or we could have had all plasma and no bacteria.
This is due to the fact that n order for the E coli. bacteria to gain either of the traits the plasmid had to be present in the first place, because the GFP gene is inside the plasmid. If arabinose is not in the media in which the bacteria was growing on then the GFP gene could not turn on, thus the bacteria can not glow. This is why the LB/amp/ara plate was the only one to express both traits(antibiotic resistance and glowing). The second part to our hypothesis was that using HIC,GFP would be purified and the final tube would express GFP under an ultra violet light.