Trypsin Research Paper
Assignment Biochemistry
Topic Industrial and Clinical Significance of Trypsin
Names Mamoona Ashraf (Roll#22) Kinza Saeed (Roll#07) BS BIOCHEM (2nd Semester) IBB
Introduction
• Trypsin is a digestive enzyme belongs to serine proteases.
It is produced by Pancreas and is found in digestive system of many vertebrates.
• It is secreted in inactive form “Trypsinogen” and is converted into active Trypsin
by the action of Enterokinase in small intestine.
• It splits proteins into Peptones and Polypeptides.
Its optimum pH is 7.5-8.5
• Trypsin hydrolyzes proteins by acting on Lysine and Arginine.
• It is usually found in 3 isoforms.
• Trypsin can withstand high temperature (50-60 Celsius) and also very low temperature.
• Due to its unique properties It is
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• For healing mouth ulcers a combination prescription is spray on the product. It
contains Trypsin, Peru Balsam and Castor oil.
Skin cells harvesting • Trypsin can be utilized to make a skin substitute which facilitates adhesion and
proper growth of skin grafts.
• Recently a research paper was published under the name of “Tissue
Engineering” in which researchers found that utilizing Trypsin as a skin forming
help was a great idea to improve the growth of skin cells.
• In Future, Trypsin may become a remedy to cure skin problems. Trypsin and Cancer
• It was recently found that over expression of Trypsin Gene in tumor cells
stimulates their growth in Culture and in nude mice.
• According to recent studies, many stomach, colon and breast cancer cell lines
secretes Trypsinogen-1 or 2 as well as unidentified serine Proteases into culture
medium.
• Lung cancer cell line is involved in the secretion of unidentified Trypsin like
Proteins.
• Stomach cancer cell line is frequently found in the secretion of active Trypsin.
• Immunohistochemical analysis shows that Trypsin is expressed at high levels in
stomach and colon cancers , but hardly in breast
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