Turnip Peroxidase Lab Report
Methods of Data Collection Measuring the independent variable: The pH (the independent variable) is being tested on the turnip peroxidase to observe the reaction rates. 5 levels of pH are required for these series of reactions so pH buffers of 3, 5, 7, 9, and 11 are to be placed in each of the waters that will be put into the cuvettes for the experiment. Measuring the dependent variable: A colorimeter must be used in order to calculate the reaction rate/absorbance level of the turnip peroxidase when the different pH levels affect it. The colorimeter can be used to measure the transfer of heat to or from an object. The colorimeter must be set to the correct wavelength setting. In this experiment, the wavelength must be set to blue so it can …show more content…
Raw data: BASELINE MEASUREMENTS (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.038 0.073 0.117 0.157 0.187 0.211 0.288 TRIAL 2 0.044 0.81 0.125 0.159 0.186 0.205 0.221 TRIAL 3 0.037 0.08 0.128 0.16 0.196 0.218 0.236 BASELINE MEASUREMENTS (TRANSMITANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 91.09 84.59 76.36 69.69 64.97 61.57 59.1 TRIAL 2 90.37 82.92 75.07 69.29 65.23 62.32 60.08 TRIAL 3 91.78 83.27 74.5 68.19 63.74 60.52 58.09 pH: 3 (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.006 0.012 0.014 0.017 0.019 0.02 0.022 TRIAL 2 0.01 0.015 0.018 0.021 0.022 0.024 0.025 TRIAL 3 0.007 0.012 0.017 0.02 0.023 0.024 0.026 pH: 3 (TRANSMITTANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 98.67 97.38 96.76 96.27 95.78 95.41 95.1 TRIAL 2 97.68 96.58 95.9 95.35 94.98 94.55 94.3 TRIAL 3 98.3 97.25 96.21 95.53 94.92 94.55 94.12 pH: 5 (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.04 0.107 0.166 0.225 0.266 0.288 0.323 TRIAL 2 0.082 1.205 0.289 0.352 0.399 0.439 0.472 TRIAL 3 0.04 0.104 0.156 0.201 0.232 0.26 0.28 pH: 5 (TRANSMITTANCE …show more content…
TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 99.05 99.12 98.71 99.57 98.65 101.98 100.75 TRIAL 2 97.89 96.32 95.89 98.01 97.99 97.83 95.42 TRIAL 3 99.38 96.19 91.58 96.53 97.09 97.11 99.31 ENZYME CONCENTRATION: 2X ENZYME (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.055 0.132 0.198 0.249 0.289 0.321 0.347 TRIAL 2 0.073 0.145 0.202 0.246 0.281 0.311 0.337 TRIAL 3 0.063 0.212 0.321 0.399 0.457 0.502 0.536 ENZYME CONCENTRATION: 2X ENZYME (TRANSMITTANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 88.19 73.8 63.42 56.37 51.43 47.79 44.94 TRIAL 2 84.61 71.57 62.86 56.74 52.36 48.84 45.99 TRIAL 3 86.56 61.43 46.72 39.93 34.9 41.51 29.12 ENZYME CONCENTRATION: 3X ENZYME (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.194 0.403 0.531 0.612 0.661 0.691 0.713 TRIAL 2 0.148 0.34 0.463 0.544 0.598 0.629 0.651 TRIAL 3 0.164 0.399 0.54 0.626 0.677 0.709 0.733 ENZYME CONCENTRATION: 3X ENZYME (TRANSMITTANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 64 39.5 29.43 24.45 21.84 20.35 19.35 TRIAL 2 71.15 45.66 34.47 28.56 25.26 23.52 22.34 TRIAL 3 68.54 39.94 28.87 23.65 21.03 19.54
J48 Unit 8 Assignment
391 Words | 2 PagesIn this experiment, we performed this experiment twice (1st and 2nd rounds) over approximately five month. Twelve male
Alka-Seltzer Experiment
853 Words | 4 PagesIdentify the independent Variable Size of the tablet Identify the dependent Variable Reaction time List the controlled variables The solution (water), temperature, volume of water (200 mls) The amount of Alka- Seltzer How will the dependent variables be measured A stop watch will be started as the pieces of Alka-Seltzer are dropped into 200ml of room temperture water Describe the expected results if the hypothesis is true The 3 smaller pieces of Alka Seltzer will dissolve quicker than the ½ tablet .
Horseradish Peroxidase Lab Report
1090 Words | 5 PagesIt was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.
Kleenex The Strongestest
81 Words | 1 PagesThe purpose of this lab was to find out what the strongest tissue of the three brands my group tested. My group 's hypothesis w.as, if different amounts of weight is put on three different brands of tissues, then the Kleenex will stay the strongest, because this brand is more advertised. The experiment concluded that Up & Up was the strongest at 28 pennies, Kleenex was the second strongest at 9 1/2 pennies, while Darnix came in last place at 5 pennies.
Catechol Oxidase Lab Report
642 Words | 3 PagesFill each cuvettes with its respective solution. Turn on the spectrophotometer, so it can warm up then calibrate it to 0% absorbance. Put the corresponding extract blank and set the spectrophotometer to 100% transmittance, then calibrate it to 540 nm. Once catechol is added in the cuvettes, make sure the solution is mixed. Place carrot cuvette in the spectrophotometer and record the resulting transmittance.
Enzyme Turnip Peroxidase Lab Report
1117 Words | 5 PagesThe three things that can cause the enzyme to denature is a large change in pH level, High Temperature, and substrate concentration. According to our knowledge, we know that a large change in pH will cause instability in the protein structure thus resulting in denaturation of the enzyme. From the data, we can see that pH 3 (total:6.3) and 10 (total:6.2) were the slowest because pH 3 is probably the highest acid and pH 10 is the highest base. The highest acid or base pH represents a large change which would cause the enzyme to denature. The fastest pH was 6 (total:34.5), and it seems that there wasn’t a large change which resulted in a stable structure.
Substrate Concentration Lab Report
181 Words | 1 PagesHypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out. Independent Variable: Substrate concentration Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube.
Spinach Leaf Photosynthesis Lab Report
1169 Words | 5 PagesBecause carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.
Lab Report On Enzyme Activity
1419 Words | 6 PagesIndependent Variable amount of substrate (sucrose) present 3. Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. I believe glucose and fructose was used as an indicator because they are what produces sucrose and sucrose creates more sucrase activity.
Laboratory Report on Enzyme Activity
1407 Words | 6 PagesIndependent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of
Cardiovascular Step Test Lab Report
157 Words | 1 PagesFor the step test, the heart rate of both participants served as the dependent variable, whereas, the independent variable was the level of training. During the experiment, the group performed 4 replications. Both participants for treatment #1 and #2 took their pulse rates prior to the test and recorded the results. At the end of 3 minutes, both participants took their pulse
Effect Of Temperature On Amylase
1167 Words | 5 PagesDependent The time taken for the bluish -black color to fade away (color of Iodine solution mix with starch solution ). The rate of enzyme reaction Minutes (min) Table 1.1 – Table shows the controlled variables in the experiment variables Units Measures of controlled variables.
Alkaline Phosphatase Enzyme Lab Report
2575 Words | 11 PagesUse these results to determine the product concentration, using Beer-Lambert’s Law: A= ɛCl (where A is the absorbance, ɛ is the molar absorptivity, C is the product concentration and l is the length of solution that the light passes through). Calculate the product concentrations at every minute for 10 minutes for all 7 of the test tubes using Beer-Lambert’s Law. Plot a graph of product concentration vs. time and then use the gradients of the 7 test tubes to determine the velocities of the reaction. After calculating the velocities, plot a Michaelis-Menten graph of velocity vs. substrate concentration.
Lab Report On Acid Base Titration
1346 Words | 6 PagesPractical I: Acid-base equilibrium & pH of solutions Aims/Objectives: 1. To determine the pH range where the indicator changes colour. 2. To identify the suitable indicators for different titrations. 3.
Reaction Between Hydrochloric Acid And Calcium Carbonate
1398 Words | 6 PagesTABLE OF VARIABLES Independent Variable Method of measurement Concentration of HCl
More about Turnip Peroxidase Lab Report
J48 Unit 8 Assignment
391 Words | 2 PagesAlka-Seltzer Experiment
853 Words | 4 PagesHorseradish Peroxidase Lab Report
1090 Words | 5 PagesKleenex The Strongestest
81 Words | 1 PagesCatechol Oxidase Lab Report
642 Words | 3 PagesEnzyme Turnip Peroxidase Lab Report
1117 Words | 5 PagesSubstrate Concentration Lab Report
181 Words | 1 PagesSpinach Leaf Photosynthesis Lab Report
1169 Words | 5 PagesLab Report On Enzyme Activity
1419 Words | 6 PagesLaboratory Report on Enzyme Activity
1407 Words | 6 PagesCardiovascular Step Test Lab Report
157 Words | 1 PagesEffect Of Temperature On Amylase
1167 Words | 5 PagesAlkaline Phosphatase Enzyme Lab Report
2575 Words | 11 PagesLab Report On Acid Base Titration
1346 Words | 6 PagesReaction Between Hydrochloric Acid And Calcium Carbonate
1398 Words | 6 Pages