Methods of Data Collection Measuring the independent variable: The pH (the independent variable) is being tested on the turnip peroxidase to observe the reaction rates. 5 levels of pH are required for these series of reactions so pH buffers of 3, 5, 7, 9, and 11 are to be placed in each of the waters that will be put into the cuvettes for the experiment. Measuring the dependent variable: A colorimeter must be used in order to calculate the reaction rate/absorbance level of the turnip peroxidase when the different pH levels affect it. The colorimeter can be used to measure the transfer of heat to or from an object. The colorimeter must be set to the correct wavelength setting. In this experiment, the wavelength must be set to blue so it can …show more content…
Raw data: BASELINE MEASUREMENTS (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.038 0.073 0.117 0.157 0.187 0.211 0.288 TRIAL 2 0.044 0.81 0.125 0.159 0.186 0.205 0.221 TRIAL 3 0.037 0.08 0.128 0.16 0.196 0.218 0.236 BASELINE MEASUREMENTS (TRANSMITANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 91.09 84.59 76.36 69.69 64.97 61.57 59.1 TRIAL 2 90.37 82.92 75.07 69.29 65.23 62.32 60.08 TRIAL 3 91.78 83.27 74.5 68.19 63.74 60.52 58.09 pH: 3 (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.006 0.012 0.014 0.017 0.019 0.02 0.022 TRIAL 2 0.01 0.015 0.018 0.021 0.022 0.024 0.025 TRIAL 3 0.007 0.012 0.017 0.02 0.023 0.024 0.026 pH: 3 (TRANSMITTANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 98.67 97.38 96.76 96.27 95.78 95.41 95.1 TRIAL 2 97.68 96.58 95.9 95.35 94.98 94.55 94.3 TRIAL 3 98.3 97.25 96.21 95.53 94.92 94.55 94.12 pH: 5 (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.04 0.107 0.166 0.225 0.266 0.288 0.323 TRIAL 2 0.082 1.205 0.289 0.352 0.399 0.439 0.472 TRIAL 3 0.04 0.104 0.156 0.201 0.232 0.26 0.28 pH: 5 (TRANSMITTANCE …show more content…
TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 99.05 99.12 98.71 99.57 98.65 101.98 100.75 TRIAL 2 97.89 96.32 95.89 98.01 97.99 97.83 95.42 TRIAL 3 99.38 96.19 91.58 96.53 97.09 97.11 99.31 ENZYME CONCENTRATION: 2X ENZYME (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.055 0.132 0.198 0.249 0.289 0.321 0.347 TRIAL 2 0.073 0.145 0.202 0.246 0.281 0.311 0.337 TRIAL 3 0.063 0.212 0.321 0.399 0.457 0.502 0.536 ENZYME CONCENTRATION: 2X ENZYME (TRANSMITTANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 88.19 73.8 63.42 56.37 51.43 47.79 44.94 TRIAL 2 84.61 71.57 62.86 56.74 52.36 48.84 45.99 TRIAL 3 86.56 61.43 46.72 39.93 34.9 41.51 29.12 ENZYME CONCENTRATION: 3X ENZYME (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.194 0.403 0.531 0.612 0.661 0.691 0.713 TRIAL 2 0.148 0.34 0.463 0.544 0.598 0.629 0.651 TRIAL 3 0.164 0.399 0.54 0.626 0.677 0.709 0.733 ENZYME CONCENTRATION: 3X ENZYME (TRANSMITTANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 64 39.5 29.43 24.45 21.84 20.35 19.35 TRIAL 2 71.15 45.66 34.47 28.56 25.26 23.52 22.34 TRIAL 3 68.54 39.94 28.87 23.65 21.03 19.54