Unit 2 Plasmid Research Paper
Instructions: Answer the questions as directed.
Upload the assignment prior to the beginning of your next lab section. Make sure you give yourself time to troubleshoot any issues you may have with uploading the assignment. You are responsible for uploading the correct file.
Given the map of the plasmid in Figure 10-3, you should be able to predict the length of DNA fragments that will result when these digests are completed.
Predict sizes of DNA fragments produced from PvuII digest:
628-306= 322
2686-322= 2364
Predict sizes of DNA fragments produced from AvaII digest: 2059-1837= 222
2686-222=2464
Predict sizes of DNA fragments produced from PvuII+ AvaII digest:
2686-2059+306= 933
2686-322-222-933= 1209
Part 5. Questions
Compose your answers with complete, grammatically correct sentences. This assignment is due at the beginning of next lab
1. For a plasmid to be useful as a recombinant DNA vector, it must have some essential features. What
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How could a single nucleotide change on the plasmid alter the fragment pattern observed in the gel? (3 points)
When a single nucleotide is change on the on the plasmid, it will make the restriction enzyme not to recognize the fragment pattern and also all the numbers at each site will be altered or this action could make the fragment not to be incised at the expected site, which could make a larger fragment to travels a short interval on the gel with less fragments than expected. It could also make it to be incised at more places than that originally expected and the result will be increased, smaller fragments that move further across the gel. There may also be no change in the banding pattern.
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