Using the skills in the microbiology lab you can discover and make a educated decision of determining the mystery microbe, with the enough supported information collected from the chemical test and stains. There will be 17 test that will be run, each of this test will bring an important clue in determining the Mystery Microbe based on their environmental
Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome.
The following was eliminated because they are gram positive: Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus megaterium, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus brevis, Geobacillus stereothermophilus, Lysinibacillus sphaericus, Clostridium perfringens, Corynebacterium pseudodiphtheriticum, Listeria monocytogenes, Lactobacillus casei, Enterococcus faecalis (Streptococcus), Streptococcus lactis (Lactococcus), Streptococcus mutans, Streptococcus pneumonia, Micrococcus (Kocuria) roseus, Sarcina lutea (Micrococcus luteus), Sporosarcina ureae, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, and Rhodococcus rhodochorous. The remaining microbes are gram negative: Alcaligenes faecalis, Rhizobium radiobacter, Salmonella typi, Serratia marcescens, Shigella flexneri, Yersina pseudotuberculosis, Erwina persicinus, Serratia liquifacens, Halobacter salinarium, Enterobacter aerogenes, Eschirichia coli, Flavobacter capsulatum,
Catalase activity test establishes whether the bacterium produces the enzyme catalase. The eosin methylene blue test or EMB, inhibits the growth of gram positive bacteria and tests whether or not gram negative bacteria can ferment lactose. Lactose fermentation testing is done to see if the bacterium is capable of fermenting sugar by testing for acid and gas production. These are the possible tests that are needed in order to identify unknown
Lockjaw, also more commonly know as tetanus or Clostridium tetani, is a desease caused by bacterial infection. the bacterium Clostridium tetani, is commonly found in soil, saliva, dust, and manure and can be introduced to the blood stream via a skin puncture, the bacterium produces a toxin called Tetanospasmin which interferes with muscle contractions, resulting in muscle spasms or spasmodic muscle contractions. (https://en.wikipedia.org/wiki/Tetanus). Tetanospasmin permanently binds receptors, so in that sense it can 't be cured. In the case of advanced tetanus treatment involves antibiotics and paralytics while being put on a respirator, which is needed until your receptors regenerate.(Andrew Lehman, Immunologist and Virologist, https://www.quora.com/How-can-tetanus-be-cured).
Selective medium involves medium with environmental conditions that specifically grows some microbes while inhibiting others. Differential medium is used to identify and differentiate (as the title says) closely related microbes based on growth responses and physical indicators. It is imperative to use laboratory positive and negative controls in identifying the unknown because it confirms and compares the results of the unknown’s response to the definite guide. While performing the procedures in this report, students had to keep the bacterial and biological species concepts in context. The bacterial species concept is the identification and naming of microbes based on relating physical and physiological features of the unknown to the fitting taxa.
The phenol-red indicator helps to identify the bacteria. Upon viewing my results it was determined that my colonies did not ferment mannitol. This was easily observed because they remained translucent. The incubation times for all of the inoculated tests were for 48 hours (due to lab schedule, supposed to be 24 hours) and the temperature they were incubated at was at 37C. 37C is the optimal temperature to maintain the state of the agar as well as the ideal temperature for continued microbial growth to occur. My fourth and final test conducted to help identify my bacteria was an Oxidase test.
It is classified as a beta-hemolytic streptococcus because when cultured on a blood agar plate all the red blood cells are ruptured by the bacteria. It is also known as beta-hemolytic group A streptococcus. Streptococcus pyogenes begins infection on the surface of the skin or in the throat but is usually found in the throat. It is the same bacteria that the causes Strep throat. This bacteria can be life threaten when it infects the fascia, or connective tissue that surrounds muscles, blood vessels and nerve.
This bacteria are multi-resistant to beta-lactam antibiotics, meaning that they are resistant to different kinds of antibiotics, such as penicillin, methicillin, dicloxacillin and cephalosporin. As the process goes, that normal Staphylococcus aureus are being killed by penicillin antibiotic, and the ones that are resistant survived and keep on reproducing, Staphylococcus aureus bacteria that is penicillin resistant spread in the community and its amount kept on increasing. This made natural selection to occur, which the bacteria that are antibiotic resistance are suitable ones and they survived in
The glycogen storage diseases are Glycogen Storage disease, types I and II. Glycogen Storage Diseases can be found by taking a blood test. These involve enzymatic deficiencies. The symptoms can be seen by muscle atrophies and abnormal liver function. Another symptom seen is low-blood sugar.
In the first four chapters of Deadly Companions author Crawford takes us on a journey on how microbes shaped our history. Starting with when microbes first appeared on planet Earth to Darwinian evolution of single celled organism. In chapter two “Our Microbial Inheritance” she discussed the relationship of microbes with hunter-gatherers and early settlements; and their relationship to disease. She looks at the impact of microbes on the lives of individuals and the population as a whole. Hunter-gatherers were small groups of people that hunted for food, moving from place to place.