Unknown Bacteria Lab Report
Introduction
Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria.
Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans). Bacteria can be classified as gram positive or negative (difference in call wall). Gram positive bacteria have a thick cell wall of peptidoglycan (“polymer of disaccharides cross-linked by shorts chains of amino acids”), and stains purple after the staining procedure under a microscope (Todar, Kenneth,
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Therefore, acid fat, lactose fermentation, mannitol fermentation were not needed to be performed, because they are selective to a specific thing. As a result, Unknown bacteria “W” was concluded to be gram stain positive, endospore positive, bacilli shape (rod shape), and arranged in chains (strepto-).
Test
Purpose
Reagents
Observation
Results
Gram Stain
To determine gram reaction of bacteria.
Crystal violet, Grams iodine, Alcohol, and Safranin
Purple streptobacilli bacteria was observed.
Gram positive rods.
Endospore
To determine if bacterium produce endospores.
Malachite green, and Safranin.
Endospore was stained in green, and parent call was stained in red.
Unknown “W” produced endospores.
The table shows that a gram stain, and endospore were the only test performed to the unknown bacteria “W”.
Flow Chart
Unknown W
Gram Stain
Gram positive Gram negative S. aureus E. coli S. epidermidis E.
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