Unknown Bacteria Lab Report

Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the

In the article “The Ultimate Social Network,” the author, Jennifer Ackerman, writes about the human microbiome. She explains the research on this and on two specific bacteria, Bacteroides Thetaiotaomicron and Helicobacter Pylori. She also talks about how these microorganisms survive in the human body.

The purpose of this experiment was to identify given Unknown White Compound by conducting various test and learning how to use lab techniques. Tests that are used during this experiment were a flame test, ion test, pH test, and conductivity test. The results drawn from these tests confirmed the identity of the Unknown White Compound to be sodium acetate (NaC2H3O2) because there were no presence of ions and sodium has a strong persistent orange color. The compound then will be synthesized with the compounds Na2CO3 and HC2H3O2 to find percent yield. Weighed 1 gram of NaC2H3O2 and mixed it with ionized water. Boiled 12 mL of 1.0M Acetic Acid added into a beaker containing the sodium carbonate on a hot plate until all the liquid is evaporated

A) All KPC Patients were isolated cleanly in separate enhanced contact room or ICU where everybody who enters the room either staff or visitors must wear gloves and gowns.

According to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC).

A milk-based, litmus broth tube is incubated and observed after 48 hours. Observations include lactose fermentation without gas as well as with gas, the reduction of litmus, casein protein coagulation and casein and protein hydrolysis. These characteristics were all determined based on the color of the solution and the production of a curd, the curds density and the production of a gas. To determine the density of the curd, the tube was slightly turned to see rather or not it was mobile or concentrated towards the bottom.

The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media.

Introduction: Transforming a gene or genetic information from one organism into another with the hopes that if done successfully the organism with the new DNA will be given new traits is a method known as genetic transformation (Rafter). Genetic transformation is used quite frequently in today’s world, form medicine to agriculture.

Escherichia Coli are bacteria found in the environment, in contaminated food and beverages and in intestines of mammals. It is the most widespread bacteria in the family of gram-negative bacteria (bacteria that can easily enter the host either by urinary and existing catheters, respirators or even wounds) called enterobacteriaceae. It is first discovered in 1885 by German bacteriologist Theodor Escherich. He was devoted in finding out what causes fatal intestinal diseases in children. He experiments by studying fetal feces through recording the organisms that are present in the intestines of healthy children and, comparing them with organisms found in sick children. As a result, he discovers rod-shaped microbes that grows at a rapid pace and

In this experiment I was testing for antimicrobial sensitivity of Staphylococcus epidermidis by using the Kirby-Bauer Diffusion test. The three antibiotics utilized in this lab were: gentamicin, novobiocin, and penicillin. I determined the effectiveness of the antibiotic by observing and measuring the zone of inhibition for each antibiotic. I used the measurements and compared them again with the Antibiotic Susceptibility zone chart. I was able to determine whether the

Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining. Approximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only

The Staphylococcus Epidermidis is classified as bacteria. Scientists reckon it to Firmicutes phylum and adjust it in Bacillales order of Bacilli class. This bacteria belongs to Staphylococcaceae family. As the name order, it is settled into Staphylococcus genus and S. Epidermidis species. S. Epidermidis makes its home on human skin, mucosal layer and nasal mucosa. Diseases can be taken form in human body and warm-blooded animals such as septicemia and endocarditis. In fact, S. Epidermidis is not too harmful on healthy tissue. The infection often occurs on newborn baby, drug users, and older people and those who need to use assistant devices on every part

INTRODUCTION: Quick look at your hands do you see them. (attention) Do I see what you might be asking? Well the millions of bacteria that are currently hanging out on your hands. (credibility) I am here today to focus in on how you can protect yourself from these invisible killers. Probably the single most repeated thing in my nursing classes has been hand washing. (rapport) You might be thinking well I am not a nursing student so this does not apply to me, but I hope to prove to you the importance of washing your hands several times a day. The problem I want to focus on is that many people don’t wash their hands, people need to wash their hands, and you should wash your hands many times a day. (preview)

Prokaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan. Bacteria usually have capsules, but archaea rarely have one. Inside the prokaryote is cytoplasm and a nucleoid. The nucleus is not enclosed inside of a membrane in prokaryotes. The cell may have appendages to adhere to certain surfaces or for motility. The prokaryotic cell is smaller than the eukaryotic cell and has different qualities that make the cell less complex than a eukaryotic cell.

After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it.