The slide was then rinsed and safranin was again used as a counterstain. Using oil immersion objective lens of the microscope, unknown #76 had only reddish-pink cells without any signs of spore formation. Thus the given unknown is a non-spore former. Bacillus subtilis was used for positive control and Escherichia coli for negative control for endospore
In part C. pairs of compounds were investigated to determine whether the compounds were miscible or immiscible. Diethyl ether and methylene chloride, diethyl ether and ethyl alcohol, water and ethyl alcohol, heptane and methyl chloride, and heptane and diethyl ether were all were found to be miscible each of these paired compounds showed a homogenous mixture with no chunks or particles left over when combined. On the other hand, water and heptane, water and methylene chloride, and water and diethyl ether were all immiscible, when mixed together it was observed that the compounds had two layers rather than one homogenous
There is no evidence that supports the symptoms of yeast infection. And there is no clinically-based statement that support candida diet as the best solution to treat any serious medical conditions. The main benefit of candida diet plan is you feel better as you cut off your sugar and flour intake and replace them with complex carbohydrates. Myths of Candida • Strict candida is a medicine A strict candidate diet plan sound too impossible to most people. This diet may improve your yeast infection, however, that does not heal you.
This lab’s end result was to correctly identify each unknown solution using prior knowledge of chemical properties and the results of the first experiment conducted. Unknown solution D was the only colored solution, being blue while the others were clear. This made it easy to then match D up to Copper Sulfate because of its color. As unknown A and B were added together, lots of gaseous bubbles formed and revealed the fact that that reaction was the reaction between Hydrochloric Acid and Sodium Carbonate because it was the only reaction that produced a gas release. Unknown A and C produced the only yellow, brown precipitate just as the reaction between Sodium Carbonate and Silver Nitrate had previously.
Based on the assumed contamination of the TLC plate and or capillary tube, it is not possible to tell whether acetaminophen was successfully separated from the Excedrin powder. The Rf values of isolated aspirin and pure aspirin were the same. This demonstrates that the aspirin was successfully separated and is relatively pure. The isolated caffeine sample had a higher Rf value but when viewed under UV light, the markings of isolated caffeine were within the bounds of the pure caffeine, leading to the conclusion that while isolated the caffeine sample was note
Methyl Red were added to MR test tube. As the solution becomes acidic it will turn red immediately. A negative result will have no color change. The VP test if the color change was red it indicated a positive results. Citrate test is used to identify if an organism is capable of utilizing citrate as a sole carbon source.
Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome. Lastly, a glucose fermentation test was conducted to determine the unknown organism.
This determined which set of tests that had to be done. My bacterium turned out to be gram positive. When conducting these tests, I only had to do the coagulase test and the catalase test because when doing the catalase test, the reaction was that it had bubbled. If it did not bubble, or have a positive reaction, then I would not have had to do the coagulase test. Also, since my bacterium caused a positive catalase test, I only had to do the coagulase test and no other tests.
Interestingly, the MICs observed for DMBQ was higher than those obtained for BQ, except when tested against B. cereus. This was due to an interesting effect of the methoxy identity of 1, 4-benzoquinones. The MIC determination of methoxybenzoquinone (MBQ) against S. typhimurium was found to be significantly lower than that of DMBQ (512 and 32 μg/mL, respectively). The difference in the number of methoxy groups in the 1, 4-benzoquinones significantly affected their antibacterial activities against the Gram-negative bacteria S. typhimurium and E. coli. Furthermore, hydroquinone (HQ), a reduced form of BQ, had significantly lower antibacterial activity than