Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Carbohydrates, lipids, proteins, and nucleic acids are organic molecules found in every living organism. These macromolecules are large carbon based structures. The macromolecules are assembled by joining several smaller units, called monomers, together through a chemical reaction called dehydration synthesis. The resulting polymer can be disassembled through the complementary process called hydrolysis.Carbohydrates are made of carbon, hydrogen and oxygen atoms in a 1:2:1 ratio. This means that for every carbon atom present in the carbohydrate there are two hydrogen
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media.
Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398.
Staphylococcus epidermidis is the organism that was identified based on the tests that I had conducted. The tests that I used to identify this organism were the coagulase test and the catalase test. My bacterium was beta hemolytic as well. First, a gram stain had to be done to determine whether the organism was a gram positive organism or a gram negative organism. This determined which set of tests that had to be done. My bacterium turned out to be gram positive. When conducting these tests, I only had to do the coagulase test and the catalase test because when doing the catalase test, the reaction was that it had bubbled. If it did not bubble, or have a positive reaction, then I would not have had to do the coagulase test. Also, since my bacterium caused a positive catalase test, I only had to do the coagulase test and no other tests. This is because with staphylococcus organisms, these are the only tests
The information was gathered through multiple tests which are used to detect the presence of certain macromolecules in the victim’s stomach contents. Then, the detective will be able to pin-point which restaurant the victim last ate at by
The purpose of quantitative analysis of protein using a spectrophotometer is to measure the concentration of proteins in a given sample. The experiment is conducted by laboratory method (Biuret Test) and using spectrophotometer to analyze the absorbance of reactants at 540 nm, hence determining the concentration of the proteins in a given sample.
This paper is based on an assessment of a patient. The assessment underlines the nutritional health status of the patient, by indicating that he is malnourished. This patient has few chronic diseases including hypertension, pernicious anemia, and depression. The mini nutritional assessment finding, labs, and interventions will be further discussed.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining. Approximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only
The Staphylococcus Epidermidis is classified as bacteria. Scientists reckon it to Firmicutes phylum and adjust it in Bacillales order of Bacilli class. This bacteria belongs to Staphylococcaceae family. As the name order, it is settled into Staphylococcus genus and S. Epidermidis species. S. Epidermidis makes its home on human skin, mucosal layer and nasal mucosa. Diseases can be taken form in human body and warm-blooded animals such as septicemia and endocarditis. In fact, S. Epidermidis is not too harmful on healthy tissue. The infection often occurs on newborn baby, drug users, and older people and those who need to use assistant devices on every part
Pseudomonas aeruginosa are rod-shaped, monoflagellated bacteria. They are in the domain Bacteria, phylum Proteobacteria, class Gamma Proteobacteria, order Pseudomonadales, family Pseudomonadaceae, genus Pseudomonas, and species P. aeruginosa. They range in size from 1-5 micrometers in length and 0.5-1.0 micrometers in width. There are 3 possible colony morphologies that can be seen and these depend on where the bacteria is taken from. P. aeruginosa from water and soil are typically small and rough around the edges. The other two morphologies are both found in clinical samples; the first is large and smooth, and is often described as having a fried-egg appearance. The second has a mucous-like appearance, which has been attributed to the
Characteristics & laboratory identification; Listeria species are gram-positive, rod-shaped, and facultative anaerobes, and do not produce endospores. in microscopy, they have an appearance of small rods, sometimes are in short chain arrangement. They may be identified in direct smears to be coccoid, therefore can be mistaken for streptococci. They may resemble corynebacteria when in longer chain. Flagella are produced at room temperature but not produced at 37℃ (normal body temperature). They are non-branching, non-capsulated as well in laboratory
Tube 1 had 1 drop, tube 2 had 2, and each tube after had an additional drop until tube 5. Next, deionized water was placed in each tube. Tube one had 4 drops; tube 2 had 3 drops and the pattern continued until tube 5. After each tube was filled with the glucose and deionized water, the contents were mixed and centrifuged. After the tubes were centrifuged, any pellets formed during the process were removed. 5 drops of Enzyme color reagent was put into each test tube and then incubated. During the incubation process, the tubes were agitated to evenly mix all the contents in the tube. Following incubation, the spectrophotometer was heated up to prepare for sample readings. Each tube was then dragged into the spectrophotometer to be analyzed. A data point for each analyzed tube was placed on the graph to show the optical density and glucose concentrations. After graphing this data, part two needed to be completed. To start, 5 different test tubes were filled with 3 drops of 5 different patients blood followed by 5 drops of deionized water. Next, 5 drops of Barium Hydroxide was placed in each tube to clear proteins and cell membranes for an accurate reading could be made. Blood Clots also interfere with the readings of glucose, so 1 drop of Heparin was placed in each tube to prevent blood clots. The tubes were mixed and centrifuged. After, the pellets were removed, 5 drops of Enzyme Color Reagent went into
Prokaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan. Bacteria usually have capsules, but archaea rarely have one. Inside the prokaryote is cytoplasm and a nucleoid. The nucleus is not enclosed inside of a membrane in prokaryotes. The cell may have appendages to adhere to certain surfaces or for motility. The prokaryotic cell is smaller than the eukaryotic cell and has different qualities that make the cell less complex than a eukaryotic cell.