Unknown Microbial # 398

The reaction that occurs can be investigated via the adding of the liver extract which contains the arginase to urea concentrations and distilled water. The amount of urea formed is determined via spectrophotometric analysis α-INPP. The urea produced was known via the color reaction with the α-INPP, it is the reagent used for the colorimetric determination of urea. (Barry J, et al. 1984). The red color formed when the α-INPP is reacted with the urea, is sensitive to light thus it is photo labile.

Exercise 1 1. Suppose a household product label says it contains sodium hydrogen carbonate (sodium bicarbonate). Using your results from Data Table 1 as a guide, how would you test this material for the presence of sodium bicarbonate? B BoldI ItalicsU Underline Bulleted list Numbered list Superscript Subscript33 Words

3. To purify and identify the product, recrystallization is used in order to purify the product, then melting point and TLC techniques are used to identify the product. Theory 4.

Investigation of Preeclampsia And Hellp syndrome Student name: Amer Binessa. Student number: D13123451 Subject: Biochemistry Lecturer: Dr Frank Clake Date:01/11/2016

Alka-Seltzer tablets are used to treat headaches, stomach and body aches and also heartburn. They consist of citric acid (C6H8O7), baking soda (NaHCO3), and aspirin (C9H8O4). The fizzing observed is a result of a chemical reaction between the citric acid and baking soda which form carbon dioxide in turn causing the fizzing. When the tablets are dropped into water they dissolve and dissociate into the ions in the equation: C6H8O7 (s) → 3H+ (aq) + C6H5O7 3- (aq).

In order to determine the value of X, the hydrate is heated on a burner to undergo decomposition reaction to be decomposed into CuSO4 and water vapor. Water vapor is evaporated during the reaction, leaving CuSO4 crystals, which is supposed to be white, in remain. By weighing the mass of CuSO4 and the mass difference of substance before and after the reaction, the mole of CuSO4 and H2O can be calculated. The value of X can thus be determined by calculating the mole ratio of CuSO4 and H2O. In the lab, through calculation, the value of X is determined to equal to 5.361211229, which is close to 5.

We isolated our crude yield while comparing 2 purification techniques: column chromatography and recrystallization. TLC, NMR, and IR spectroscopy were used throughout the process to identify ferrocene and acetylferrocene in addition to evaluating the levels of purity. Evidence: The objective of our experiments was to prepare acetylferrocene from ferrocene. The overall reaction was carried out using 6.1 equivalents of liquid acetic anhydride to 1.8 equivalents of phosphoric acid and concluded with an aqueous workup with NaOH.

Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.

Purpose: The purpose of this lab is to titrate an unknown solid acid (KH2PO4) with a standardized sodium hydroxide solution. After recording and plotting the data, the acid’s equivalence point will be recorded once the color changes. Using the equivalence point, the halfway point will be calculated, which is used to determine the acid’s equilibrium constant. The acid’s calculated equilibrium constant will be compared with the acid’s established pKa value.

Before haem iron can be absorbed, it must be hydrolysed from the globin part of haemoglobin or myoglobin; this is carried out by proteases in the stomach or small intestine. Once the haem is released from the globin, it is absorbed across the mucosal cells of the small intestine by haem carrier protein 1 (HPC1). Once absorbed, the haem molecule is hydrolysed into inorganic ferrous iron and protoporphyrin by haem oxygenase, and can be used by the intestinal cell, excreted or used by other tissues. Non haem iron must be released from food components in order to be absorbed, this process is aided by gastric secretions such as hydrochloric acid and proteases in the stomach and. Following its release from food, the non-haem iron is present in its ferric form in the stomach.