He then demonstrated that the crystalline form differed from one animal species to another. Using his own newly constructed gas pump, he found that oxygen formed a loose, dissociable compound with hemoglobin, which he called "oxyhemoglobin." He renamed the iron free hematin ‘hematoPorphyrin’. Henceforth, researches were carried out on porphyria by various researchers. Ref: http://www.porphyriafoundation.com/about-porphyria/history-of-porphyria 2.
Patient’s laboratory findings were ; creatinin: 1.02 mg/dl, albumin: 2.9 mg/dl, Total bilurubin: 4.0 mg/dl, ALT:22 IU/L AST:20 IU/L, hemoglobin:10.6 gr/dl, platelet: 232.000, protrombin time: 18.7 sn, INR: 1.37, serum protein elektrophoresis: beta-gamma bridging, serum-ascites albumin gradient: 2.1 gr/dl, AFP: 6000 IU/ml, CA 19.9-CA 125-CEA: negatif, HBsAg (+), HBeAg (-), HBV DNA: 61.700 IU/ml, HDV (-), AntiHCV (-), markers for otoimmun hepatites and other etiological tests were negative. The patient was diagnosed as chronic dekompansated liver parenchymal disease due to ethanol taking and chronic heatitis B (HBV) infection. His Child-Pugh score was 9.0 (B) and MELD score was 15. He had not hepatic encephalophaty and spontaneous bacterial peritonitis infection. Stage 2 oesophageal varices were present at his gastroscopic study.
To the supernatant charcoal at the rate of 6g/liter was added to ingest the broke up ethancridine lactate. The supernatant containing immunoglobulin was at last sifted through the channel press at a weight of 70-100 Kpa. 2) purification of hyperimmune serum utilizing Ammonium sulfate as hastening
The anticancer activity of samples on HepG2 was determined by the MTT assay (Mosmann 1983). Cells (1 × 105/well) were plated in 1ml of medium/well in 24-well plates (Costar Corning, Rochester, NY). After 24, 48 and 72 hours incubation the cell reaches the confluence. Then, cells were incubated in the presence of various concentrations of the samples in 0.1% DMSO for 48h at 37°C. After removal of the sample solution and washing with phosphate-buffered saline (pH 7.4), 200µl/well (5mg/ml) of 0.5% 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl--tetrazolium bromide cells (MTT) solution was added.
 found for transesterification of soybean oil over nano CaO assisted by microwave irradiation, the optimal reaction took place at a methanol to oil molar ratio of 7, amount of catalyst of 3.0 wt.% and temperature 60oC, achieving a conversion of 96.6% at 60 min. Interestingly, they observed effect of water in methanol on the catalyst and revealed that water adsorbed dissociatively on CaO surface to form hydroxyl groups and then produced more reactive methoxy anions. Similarly with the present work, water adsorption occurred during air exposure to form Ca(OH)2 as the main component in the hydrated-calcined seashell. Methoxide anion is strong basic and has high catalytic activity in transesterification reactions . By this mechanism, therefore, the hydrated-calcined seashell effectively catalysed the palm oil conversion to biodiesel under microwave irradiation with yield of biodiesel close to equillibrium at a very short reaction time 5-10 minutes regardless catalyst
Faculty of Pharmacy Analytical Chemistry Department Assignment Topic: Chlorides of group || cations Course Title: Analytical Chemistry 1 Course Code: PC123 Lab Group: D2 Summited To: Dr.Omnia Ali Prepared By: Ahd Mohamed Abdelmoniem Fahmi-175063 Due Date: 2-4-2017 Chlorides of group || cations contain (Cadmium chloride, Mercuric chloride, Copper chloride and Bismuth chloride). 1- Mercuric chloride HgCl2:- It's poisonous odorless white crystalline solid, very toxic compound, and it's slightly volatile at room temperature. Parent acid and base: Hydrochloric acid HCl + Hg (OH) 2
“Simultaneous determination of Neomycin sulphate and Polymyxin B sulphate by Capillary Electrophoresis with Indirect UV detection”  Stationary Phase: fused silica capillary with 50 μm i.d. and 27 cm total length at an applied voltage of 6 kV Mobile Phase: 15 mM phosphate run buffer (pH 5.0) containing 40 mM N-(4-hydroxy-phenyl)acetamide and 50 mM tetradecylammonium bromide (TTAB) Detection: 280nm 7. “Flow Injection Chemiluminescence determination Of Neomycin in Pharmaceutical Formulations”  Procedure: The chemiluminescence reaction is based on the enhancing effect of neomycin on the chemiluminescence reaction between luminol-hydrogen peroxide catalysed by potassium ferrocyanide in the Triton X-100 alkaline media. Neomycin solution was injected into a water carrier stream and then mixed with hydrogen peroxide in the presence of Triton X-100 solutions. The reaction mixture was then merged at a Y-piece with a reagent stream consisting of potassium ferrocyanide and luminol in alkaline solution.
• Injection volume: 20μl • Injection concentration: 1mg /ml • Detection wavelength: 280nm 2.3.6-Isolation and purification of Flavonoids (Genistien, Rutin, Quercetin) by: 18.104.22.168- Preparative TLC plate: Isolation and purification of genistein,rutin, quercetin were carried out by preparative TLC. On a glass plates (20 cm x20 cm) a slurry of 60 gm of silica gel GF 254 suspended in 120 ml of distilled water was applied in 0.75 mm thickness manually by using Jobling laboratory division plate coater. The freshly coated plates were left until the transparency of the layer disappears. After 10 minutes, the plates heated for 1 hour at 110ºC. The completely dried and activated plates are kept in a dry place for use.
Briefly, 1.5 ml of DPPH solution (0.1 mM, in 95 % ethanol) was incubated with different concentrations of unirradiated and irradiated CMCS solutions. The reaction mixture was shaken well and incubated for 15 min at room temperature and the absorbance of the resulting solution was read at 517 nm against a blank (control). Ascorbic acid was used for comparison as antioxidant materials. The radical scavenging effect was measured as a decrease in the absorbance of DPPH and can be calculated using the following
Coagulase test Coagulase is an enzyme that causes plasma to clot by activating prothrombin to form thrombin which then catalyzes the activation of fibrinogen to form fibrin. Coagulase test was performed one drop of citrated human plasma was added on a slide by using a plastic loop or wooden stick,. Mix well and clumping was observed within 10-15 seconds indicate the coagulase positive test. Citrate test- Fresh (16- to 18-hour) pure culture was used as inoculation source. A single isolated colony was slightly streak on the surface of the simmon citrate agar slant.