Premixed and degassed solution of water and acetonitrile in the ratio of 30:70 was used as diluent. The run time for standard was kept as 6min and for sample and blank as 20 minutes. Column oven temperature was maintained at 30°C. Injection volume was 50 µL. The control of the HPLC system and data collection was by Empower software.
Mixed Phosphate Buffer 5. Lamivudine LAMIVUDINE TRAIL METHOD-I Mobile phase Acetonitrile : Water (50:50) λmax 270 nm Flow rate 1mL/min Column Inertsil C8 150 x 4.6 mm Retention Time 1.800 min Efficiency 2030 Preparation of mobile phase: Take 100ml of Acetonitrile in volumetric flask and dissolve it in 100ml of water. Then it is sonicated it for 10mint. TRAIL METHOD-II Mobile phase Water : Menthol (40:60) λmax 270 nm Flow rate 1mL/min Column Inertsil C8 150 x 4.6 mm Retention Time 1.783 min Efficiency 1294 Preparation of mobile phase
Ci(mg/L) Ce(mg/L) % extraction 95.24 2.45 97.42 125.9 3.04 97.58 329.7 2.95 99.10 1955 0.43 99.97 As shown from table 4 increasing the initial concentrations of iron (III) from 95.24 to 1955 ppm has increased % extraction. 4.5. Effect of the diluents on the extraction of iron (III) In order to get most of the MIBK dissolved in both Conc. HCl and water we used diluents such as kerosene, Benzene and Xylene. Table 5 shows
Assay of market formulation 20 tablets were accurately weighed and crushed into a fine powder. The powder equivalent to 50 mg was taken in 100 ml volumetric flask. About 50 ml methanol was added and then sonicated for 8 mins.Then the volume was finally made up to the mark (100ml).The solution was filtrated through whatmann filter paper. Then 0.6 ml of solution was further diluted to 10ml with mobile phase to get final sample concentration. Standard and sample solutions were injected five times and respectively to get the chromatograms.
Comparing with males, only 0.0267 % of triptolide can be detected in female rat urine, while no unchanged parent drug can be found in the feces or bile, which may be attributing to the different types and levels of metabolism enzymes for triptolide . A non-linear kinetics for
184.108.40.206 Sodium hypochlorite- Chloroform Method • Centrifuge culture at 8,000rpm for 20mins in prewashed tubes with chloroform and ethanol. • Discard the supernatant. • Pellet was treated with 5ml Sodium Hypochlorite and incubated at 300C for 2hrs. • Centrifuge at 5,000rpm for 15mins. • Discard supernatant and add 5ml distilled water to the pellet and centrifuge at 5,000rpm for 15mins.
Immediately following the mixing of Nitric Oxide and Peroxide, UV measurement is obtained for the resulting peroxynitrite solution. In order to make the nitric oxide solution, nitric oxide is dissolved in, previously made, oxygen free water after it has been bubbled through a 10% KOH solution. Then nitric oxide is added to a vial which is being stirred and which already contains peryoxynitrite and the reaction solution. The addition of nitric oxide results in chemiluminescence which is then recorded by a photomultirecorder. In rat kidneys the perfusion rate and flow rate of renal effluent are both set to 2ml/min, and the chemiluminescence probe is pumped into the rat kidney at a rate of 0.5 ml/min.
Then, Fe(NO3)3·9H2O (0.5mol) was added to above solution and was kept at 80 °C for 12h under reflux. The obtained red-brown solid was filtered and washed for three times with methanol and DMF, then dried in air. The as-prepared photocatalyst was characterized by SEM (VP-1450), EDX (type
Elemental sulphur has been noted as the end product of pyrite oxidation in acid media (low pH), but it is unstable in alkaline solutions. Thiosulphate was detected as an intermediate in anodic dissolution of pyrite (Mishra and Osseo-Asare, 1988). Studies conducted by Mycroft during electrochemical oxidation of pyrite (Mycroft et al., 1990) showed the formation of sulphur and polysulphide in near neutral aqueous solutions. However, in alkaline solutions, the formation of sulphur and polysulphide is only detected at higher potentials. On the contrary, Hamilton and Woods (Hamilton and Woods, 1981) operating at pH 9.2 suggested formation of monolayer sulphur during pyrite oxidation a potential of about 0.0
The typical reaction of transesterification was carried out in a 50 ml round bottom flask, attached with reflux condenser over magnetic stirrer. Jatropha oil and methanol were added in required molar ratios and heated at set reaction temperature. After attaining the reaction temperature catalyst was added to reaction mixture and time was noted as reaction start time. Stirring speed was maintained at 1000 rpm to keep system uniform in temperature and suspension. After the reaction is completed, the product mixture was rotary evaporated to separate methanol and methyl esters.