One mole of Ammonium dichromate will give rise to one mole of 1 mole of Chromium (III) oxide and 1 mole of Nitrogen gas and 4 moles of Water is gaseous phase. To convert these into formula units, 1 mole of any compound will equal 6.022X1023. So based on this, 1 mole of Ammonium dichromate is 6.022X1023 formula units. 1 mole of Chromium (III) oxide is 6.022X1023
The equation for this reaction tells that for every mole of magnesium reacted, the amount of hydrogen gas produced will also be one mole. Looking at just the first trial, after the 0.0388 g of magnesium was finished reacting in an excess of hydrochloric acid, the amount of gas produced was 42.7 mL. The number of moles of magnesium reacted, which is the same as moles of hydrogen produced, was 0.00160 moles, found by dividing the mass of magnesium by its molar mass. This, along with the temperature and pressure of the room, could them be used to find the volume of gas at STP; however, this only after taking into account the water vapor pressure in the eudiometer, which is by 23.8 was subtracted from the barometric pressure of the room. Using the combined gas law, the calculated volume of the gas at STP would be 0.0377 liters.
The solution was stirred at room temperature for 8h. The solvent was blown out with nitrogen. The residue was added to 1 ml of water containing 0.1% TFA and purified on RP-HPLC. Massspec of the final product clearly indicates presence of RB modified on PEI by series of peaks matching different polymer compositions (see Fig. 6).
The fragmentor and collision energy conditions were calculated for [M+4H]4+ m/z 698.1 and m/z 703.2 and [M+5H]5+ m/z 558.7 and m/z 562.8 for hepcidin and the [13C18, 15N3]-hepcidin standard, respectively. These were found to be the most intense ions, in accordance with Bansal et al.. We selected the transitions 698.1 → 86.1 and 562.8 → 70.2 to quantify hepcidin levels in the plasma samples of triathletes. Figure 1 shows the chromatograms of both the 10 nM hepcidin standard and the endogenous hepcidin present in the
The quenching experiments may give further information about the binding ability of NSs with Cys. Subsequently, for quenching measurements, the appropriate volume of Bi2S3 NSs and a series of Cys standard solutions were added to the 10 ml test tube, diluted by water and homogenized for determination. It was reported that the fluorescence intensity was quenched by the addition of Cys solution. The detection spectra were obtained in the range of 350–650 nm with the excitation wavelength of 350
s q. s q. s q. s q. s q. s Table no.1 Formulation Table Table No.2: Physicochemical properties of the prepared buccal patches of Metoprolol Tartrate Formulations Folding Endurance* Tensile Strength* (%) Percentage Elongation* (%) Percentage Moisture absorption* (%) Percentage Moisture loss*(%) Drug Content* (%) F1 315 ± 2.516 38.72±1.10 143±1 3.89±0.67 1.5±0.82 91.64±1 F2 326 ± 2.081 46.71±1.505 122±1 4.19±0.75 1.4±0.97 94.60±1.174 F3 328 ± 2.645 42.43±0.735 121±1 5.11±0.94 2.1±0.65 93.25±0.960 F4 318 ± 1.527 49.34±1.01 142±1 3.75±0.81 1.3±0.23 97.80±0.990 F5 345 ± 2.081 46.93±2.16 125±1 4.69±1.32 2±0.71 90.60±0.995 F6 358 ± 2.081 51.31±1.00 134±1 3.45±0.84 1.5±0.84 89.03±1.000 *Data expressed as mean ± SD
Selection of wavelength A UV spectrum of drotaverine hydrochloride, ethamsylate, tranexamic acid in water was noted by scanning the solution in the range of 200-400nm. drotaverine hydrochloride, ethamsylate, tranexamic acid was showing significant absorption at 220nm. thus that was selected as wavelength for analysis. Preparation of standard stock solution The standard stock solutions 100mg/ml each of drotaverine hydrochloride, ethamsylate, tranexamic acid was prepared than solution having concentration in range of 80-250µg/ml were prepared by diluting stock solution with mobile phase. Preparation of sample
Table 2: Effect of Pronase Treatment of the Phosphoprotein Derived from the Synaptosome-Enriched Fractiona5. Treatment Total dpm in the Total dpm in the supernatant ( S ) phosphoprotein residue [S/(S + R)] 100 after digestion of after digestion the phosphoprotein ( R ) 33P 32P 33P 32P 33P 32P Pronase 180 684 30 173 86 78 Control 8 72 773 3 8 Alkaline Phosphatase 1571 886 176 109 90 89 Control 224 122 1445 1053 13 10
The regression coefficient value of zero order kinetic plot was found to be 0.974 and the slope was found to be 3.862 (figure 14). Figure 14: Zero order plot for Optimized formula First order kinetics plot: Here the graph is plotted between log cumulative percent drug remaining Vs time. Regression coefficient is calculated and interpreted. The regression coefficient value of this plot was found to be 0.955 and the slope was found to be 0.046 (figure15). Figure 15: First order plot for Optimized formula Higuchi model: In this model, graph is plotted between cumulative percent drug released Vs square root of time.