Vitamin C Titration Lab Report

We then added 10cm3 ethanoic anhydride to the salicylic acid and swirled the contents, this mixes together the two chemicals. We then added 5 drops of concentrated sulphuric acid to the flask and thoroughly swirled the mixture, this creates the solution that makes the aspirin. We then warmed the flask for 20 minutes in a 400cm3 beaker of hot water which was approximately 60°C, we made sure the flask did not go above 65°C because this could have caused the contents to evaporate. Part 2: Using a 25cm3 measuring cylinder we measured out 15cm3 of ethanol into a boiling tube and then prepared a beaker half filled with hot water at approx. 75°C, we got this temperature by filling the beaker with cold water and slowly adding boiling water from a kettle until we reached the right temperature.

The retention factor for each dye was calculated. F or each of the Kool-Aid flavors, 2.0 g was weighed out from the packet and 5mL of water was mixed in with them each. mL of 0.1% NaCl solution was added to 100mL of bottled water. The six chromatography strips

Titration is a method that is used to determine the concentration of a solution by allowing certain volume of analyte to react with other concentration-known solution. It is important to know the point where enough titrant is added to react with the analyte, and this point is called the equivalence point. The equivalence point is shown by an indicator solution, which is a substance that changes color of the titrant at the equivalence point. However, in this experiment we are interested in finding the amount of titrant, rather than the equivalence point. There are several ways to determine the concentration of vitamin C, and redox reaction is one of the ways to figure out the concentration.

Repeat the experiment. The cola drinks were titrated using the following method: Prepare the beverage in a 250ml volumetric flask. Use a funnel to facilitate the process. Place the beaker on a hot plate so that it boils and place a watch glass on top to prevent the carbon dioxide from the atmosphere getting dissolved in the cola. Once the cola starts to boil, continue to boil it for another 10 minutes so that the carbon dioxide is removed.

Procedure First, five standard solutions were made using 10-, 20-, 30-, 40-, and 50-mg/L of caffeine. The 10-mL solution was created by mixing 10-mL caffeine standard and 10.0-mL 0.10M HCl and diluting to 100.0-mL. This same procedure was used to make the rest of the solutions. Then 6.0-mL of soda, with the carbon dioxide removed, was placed in a 100-mL volumetric flask along with 10.0-mL 0.10m HCl and diluted. Then, the UV absorbance of the five standards was taken.

MDA level was determined by thiobarbituric acid reactive substances (TBARS) in serum, based on the reaction between MDA and TBARS. Standard Malondialdehyde solution in 5 mL of volume was processed along with test samples. 1.5 mL of 0.8% of TBA was added to 1 mL of the serum sample. Then 0.4 mL of 8.1% sodium dodecyl sulphate and 1.5 mL of acetic acid was added. The mixture was finally made upto 5 mL with distilled water and placed in hot water bath at 95ºC for 1 h. After cooling, 1 mL of distilled water and 5 mL of the mixture of n-butanol and pyridine (15:1, v/v) was added.

To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water. Next, I dye the Unknown with Gram’s iodine to create a complex only have on gram positive. The slide is rinsed by water after 30 seconds. Decolorization is the next step of the whole process. I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface.

Then, putting of choloform in amount of 10 mL and solution of Hanus iodine as amount of 10 mL into conical flask is realized. Addition of these two substance into otheraflask also occurs for blank. Next, waiting for these two samples for exactly 30 minutes is realized. Afterthat, solution of potassium iodine in amount of 15 mL and 40 mL water being distilled are added. Titration of last mixture is performed in company with 0.1 M Na2S2O3 until the obtaining of color in yellow.

In addition, phenolphthalein was added as an indicator. The aliquots were titrated against sodium hydroxide (NaOH) solution until end point was reached, after which volume of NaOH consumed was recorded. The value of the rate constant, k, obtained was 0.0002 s-1. The experiment was then repeated with 40/60 V/V isopropanol/water mixture and a larger value of k = 0.0007 s-1 was obtained. We concluded that the rate of hydrolysis of (CH3)3CCl is directly proportional to water content in the solvent mixture.

Thyme tea: To help restore energy, Pour 1 teaspoon of dried thyme into 1 cup of hot water and let it soak for 5 minutes with the lid covered. Strain the content and add very little cayenne pepper to it. Add some lemon juice from ½ lemon and drink it in the morning or late afternoon to boost your energy. Electrolyte sports drinks: Sometimes fatigue is a sign of your body lacking electrolytes so get some sports drinks that have a lot of potassium and sodium for some relief. Gin-zing tea: This is an Asian tea that can boost your sagging energy up.