Vitamin C Titration Lab Report

We then added 10cm3 ethanoic anhydride to the salicylic acid and swirled the contents, this mixes together the two chemicals. We then added 5 drops of concentrated sulphuric acid to the flask and thoroughly swirled the mixture, this creates the solution that makes the aspirin. We then warmed the flask for 20 minutes in a 400cm3 beaker of hot water which was approximately 60°C, we made sure the flask did not go above 65°C because this could have caused the contents to evaporate. Part 2: Using a 25cm3 measuring cylinder we measured out 15cm3 of ethanol into a boiling tube and then prepared a beaker half filled with hot water at approx. 75°C, we got this temperature by filling the beaker with cold water and slowly adding boiling water from a kettle until we reached the right temperature.

Chromatograms where made for the known FD&C and for the three Kool-Aid samples. The retention factor for each dye was calculated. F or each of the Kool-Aid flavors, 2.0 g was weighed out from the packet and 5mL of water was mixed in with them each. mL of 0.1% NaCl solution was added to 100mL of bottled water. The six chromatography strips

Also, vitamin C is a necessary nutrient to humans; it is crucial to maintain the connective tissues and bones in the human body (Helmenstine) . Titration is a method that is used to determine the concentration of a solution by allowing certain volume of analyte to react with other concentration-known solution. It is important to know the point where enough titrant is added to react with the analyte, and this point is called the equivalence point. The equivalence point is shown by an indicator solution, which is a substance that changes color of the titrant at the equivalence point. However, in this experiment we are interested in finding the amount of titrant, rather than the equivalence point.

The cola drinks were titrated using the following method: Prepare the beverage in a 250ml volumetric flask. Use a funnel to facilitate the process. Place the beaker on a hot plate so that it boils and place a watch glass on top to prevent the carbon dioxide from the atmosphere getting dissolved in the cola. Once the cola starts to boil, continue to boil it for another 10 minutes so that the carbon dioxide is removed.

Procedure First, five standard solutions were made using 10-, 20-, 30-, 40-, and 50-mg/L of caffeine. The 10-mL solution was created by mixing 10-mL caffeine standard and 10.0-mL 0.10M HCl and diluting to 100.0-mL. This same procedure was used to make the rest of the solutions. Then 6.0-mL of soda, with the carbon dioxide removed, was placed in a 100-mL volumetric flask along with 10.0-mL 0.10m HCl and diluted.

MDA level was determined by thiobarbituric acid reactive substances (TBARS) in serum, based on the reaction between MDA and TBARS. Standard Malondialdehyde solution in 5 mL of volume was processed along with test samples. 1.5 mL of 0.8% of TBA was added to 1 mL of the serum sample. Then 0.4 mL of 8.1% sodium dodecyl sulphate and 1.5 mL of acetic acid was added. The mixture was finally made upto 5 mL with distilled water and placed in hot water bath at 95ºC for 1 h. After cooling, 1 mL of distilled water and 5 mL of the mixture of n-butanol and pyridine (15:1, v/v) was added.

Next, I dye the Unknown with Gram’s iodine to create a complex only have on gram positive. The slide is rinsed by water after 30 seconds. Decolorization is the next step of the whole process. I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface. Lastly, the unknown is once again dyed with safranin for 1 minute then wash it off with water for the last time and dry it using bibulous paper.

Then, putting of choloform in amount of 10 mL and solution of Hanus iodine as amount of 10 mL into conical flask is realized. Addition of these two substance into otheraflask also occurs for blank. Next, waiting for these two samples for exactly 30 minutes is realized. Afterthat, solution of potassium iodine in amount of 15 mL and 40 mL water being distilled are added. Titration of last mixture is performed in company with 0.1 M Na2S2O3 until the obtaining of color in yellow.

In addition, phenolphthalein was added as an indicator. The aliquots were titrated against sodium hydroxide (NaOH) solution until end point was reached, after which volume of NaOH consumed was recorded. The value of the rate constant, k, obtained was 0.0002 s-1. The experiment was then repeated with 40/60 V/V isopropanol/water mixture and a larger value of k = 0.0007 s-1 was obtained. We concluded that the rate of hydrolysis of (CH3)3CCl is directly proportional to water content in the solvent mixture.

Thyme tea: To help restore energy, Pour 1 teaspoon of dried thyme into 1 cup of hot water and let it soak for 5 minutes with the lid covered. Strain the content and add very little cayenne pepper to it. Add some lemon juice from ½ lemon and drink it in the morning or late afternoon to boost your energy.

Before starting the heating process, measure the weight of the crucible with its cover first and then tare the balance, and after that adding about 1 gram of the sample to the crucible with its cover, and then weigh it. Moreover, it is possible liberating harmful gases during the process of heating; therefore, being careful is important. The heating process ends when this sample changes the color to brown because water of hydration is removed to the sample. Additionally, give time to the small cool down and measure its weight. Next, transfer the sample to a 50 mL beaker and mixes with distilled water, which gets by rinsing the crucible with its cover in 8mL, so the solution is generated.

50 μL of these dilution solutions were separated on the TLC plate coated with SNISG. The plate was developed with petroleum ether: ethyl acetate (4:1) and the movement of solvent was usually controlled at 1 cm from the upper edge. After completion, the plate was dried until no solvent smell remained. It was sprayed with an ethanol solution containing 10% sulfuric acid, and heated at an infra-red drier until obvious color came up, as shown in Fig.2 (B.ab). Simultaneously, the amount of silver nitrate in the impact of isolative effect was investigated with the sample procedure, as shown in Fig.2

The substances used during the experiment have some hazardous drawbacks. Some hazards of working with trans-cinnamic acid are skin, eye, and respiratory irritation (CINNAMIC ACID). This compound may be combustible at high temperatures. Some hazards of working with acetic acid severe skin burns and eye damage Acetic acid). This compound is combustible.