Hydrochloroauric Acid Research Paper

CHAPTER 2

MATERIALS & METHODS

2.1 MATERIALS

The leaves from Vitex negundo (Family: Lamiaceae) were collected from agricultural field located at Tiruchirappalli. Epirubicin Gift Sample received from Venus Medicine Research Centre, Baddi, Himachal Pradesh. Hydrochloroauric acid (HAuCl4), Folic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was obtained from Sigma-Aldrich Chemicals (USA). Arabic gum was obtained from

SD Fine-Chem Limited, Mumbai (India). A549 cell lines were obtained from National Center for Cell Science (NCCS), Pune, (India). Millipore milli Q water was used for all the experiments.

2.2 CALIBRATION CURVE OF EPIRUBICIN IN PHOSPHATE BUFFER pH 7.4

100mg of drug was weighed and dissolved in 100 ml distilled water (stock). 1 ml of resulting solution was taken and dilute with 100 ml with phosphate buffer pH 7.4. Further, 1ml, 2ml, 3ml, 4ml & 5ml was taken from above diluted solution and dilute it to 10 ml with phosphate buffer pH 7.4. The absorbance of resulting solution was measured at 480 nm. The absorbance values were graphically represented in Figure.

The extract was filtered (vacuum filter) and stored at 4°C for further experiments.

2.4 GREEN SYNTHESIS OF GNPs USING LEAF