Thereafter we see an exponential increase in the production of carbon dioxide. This means there is a great activity in the break-down of the two sugar substrates by yeast. The control of this experiment lacked sugar but had two times the yeast; the yeast alone produced carbon dioxide that occupied the 5 mm subtracted from the experiment. The minute amounts of carbon dioxide that are produced result from fermentation undergone by yeast cells using the sugars that are already in their system (Barnett and Lichtenthaler,
Introduction Metabolism is the sum of all anabolic and catabolic reactions within a living organism to sustain life. The energy required to perform these reactions is provided by oxygen in the form of ATP, therefore the oxygen consumption rate can be measured to determine the metabolic rate. Since oxygen is obtained through respiration, the efficiency of an organism’s respiratory system affects its metabolism. Previous studies have shown that caffeine affects the human respiratory center and occasionally dilates bronchus. It can thus stimulate human respiration and increase the metabolic rate (Haggins et al, 1915).
All colour changes were recorded. Next, the test tubes were carefully cleaned with soap and water. Then five millilitres of sample “A” was placed in the test tube labeled “A”. This was then repeated with the next three samples. Then a few drops of each sample were placed on glucose/ketone paper.
These are examples that can decomposition of the hydrogen peroxide. The temperature of the liver The surface area of the liver The Ph. of the hydrogen peroxide The concentration of the enzymes The variables I am going to look at are, different Temperatures in hot water baths, and one with an ice bath. My Hypothesis for this experiment would be that the temperature of the liver enzymes would react best at, 35-40 degrees, because the enzyme in the liver wouldn’t exist after 40 degrees Celsius I know this because of this is the experiment that I have just carried out, as soon as the rate goes over every ten degrees Celsius the liver is warmed up but when it goes over 40 Celsius the rate of decomposition will slow it down, So reaching the highest Celsius in my experiment would be 60 Celsius so therefore the enzyme really wouldn’t exist. There for my enzyme activity would be at its greatest in room temperature.
ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. In this experiment we examined how increasing the volume of the extract added to the reaction would affect the rate of the reaction. The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. Using different pH levels, the absorbance rate of the reaction was measured to see at which condition the enzyme worked best. The rates of absorption were calculated using a spectrophotometer in 20 second intervals up to 120 seconds.
500gms of fresh beetroot or its hairy root has to be homogenized with sand continuously for three times in 70% ethanol (100mg/L).Then extracts will be centrifuged at 10xg for half an hour and supernatants will be allowed to vaporise at 40°C under vacuum till they get dried. Then, the residues will be dissolve in 0.5L of 70% of methanol and this sample-methanol mixtures keep in refrigerator at -20 degrees for 24hr, consequently thawed and supernatants are carefully collected from the precipitate. Under vacuum, methanol will be evaporated, at 40°C, from the supernatants and following betalains are lyophilized from aqueous fractions and finally 6.5gms of dry betalains will be obtained (Christ, Alpha 1-2,
This experiment is set up the study the effect of different sodium bicarbonate concentrations on the rate of photosynthesis in spinach (Spinacia oleracea). Photosynthesis is the process by which plants and other photoautotrophs synthesize organic compounds from carbon dioxide (Faculty of Science and Horticulture, 2018). Photosynthesis takes place in the chloroplast of a plant cell, where sunlight, water, and carbon dioxide are used in a reaction to produce oxygen and sugar (Reese 2017). In the photosynthesis reaction carbon dioxide is reduced to make sugar and water is oxidized to make oxygen. In this experiment oxygen production is being used to measure the rate of photosynthesis.
If the ratio is close to 1.0, the subject is most likely in extreme exercise and utilizing only carbohydrates. The ratios of oxygen and carbon dioxide are shown through the oxidation reactions of both fat and carbohydrates. It is possible to calculate an RER higher than 1 because of hyperventilation in the lungs . The respiratory quotient (RQ) is the measurement of CO2 and O2 in the tissues at the cellular level. The most accurate way to determine RQ is through the bicarbonate buffer reaction where the amount of hydrogen ions show metabolism.
In this experiment, a balanced chemical equation will be provided for decomposition reactions. A redox reaction is a reaction where one reactant is reduced due to a gain of electrons, and the other is oxidized due to a loss of electrons. The reactant that loses an electron is called the reducing agent, whereas the reactant that gains an electron is called the oxidizing agent. In this experiment, the balanced chemical equation, and the two half equations for the redox reactions will be provided, and the oxidizing and reducing agents will be
To calculate the percentage of Cu, we divided the final mass of the penny 0.09 and the initial mass of 2.47 and multiplied by 100. To calculate the percentage of Zn, we divided the final mass of the penny 2.38 and the initial mass of 2.47 and multiplied by 100. During the experiment the hydrochloric acid donated its hydrogen ions in the reaction and then the chloride ions reacted with the zinc ions in the solution. Thus, the zinc dissolved in the highly acidic solution which was caused by the high concentration of H2 ions. Hydrogen gas was generated during the reaction which was seen when bubbles were formed as the penny was dissolved into the beaker.