Osmosis Lab Report Research Question: How does the change in the concentration of a sucrose solution affect the process of osmosis in a potato cell by measuring its mass? Background information: 1 Osmosis is the process by which a liquid passes through a semi-permeable membrane, moving from an area with a high concentration of water to a low concentration of water. There are various factors that affect osmosis such as: concentration, surface area and temperature. The concentration of solutions can affect the rate of osmosis, as there is more difference in the concentration of the solutions, which means osmosis, will take place quicker. Surface area could affect osmosis based on the ease by which molecules can get through the semi-permeable
Figure 2. Column chromatography set-up After setting up the column, 2 10-ml of the chosen solvent was obtained and was placed in two separate test tubes. Using a dropper, ~0.5 mL of the food dye was put into the column by dropping it at the side of the column in a circular motion. The chosen solvent was then added just after the green food
You may need a spoon to make the layer as even as possible. Cover the filling with the rest of phyllo dough after you have brushed each phyllo with butter again. Preheat oven to 374°F (190°C). Brush well the surface of melitzanopita with butter, carve its surface with a knife and sprinkle some sesame. Bake for around 55 minutes or until its surface gets a nice golden brown color.
Higher Concentration of Sucrose Lowers the Mass of a Potato Independent Variable: Concentration of Sucrose used Dependent Variable: Mass of each Potato after Experiment Constant: Size of Potato being used at room temperature Introduction We learned about hypertonic and hypotonic environments before this lab as well as what takes place during osmosis. Do potatoes loose or gain mass when soaked in specific solutions such as sugar or salts? Sugar is a large molecule and has low permeability. Potatoes are a starch which means it is composed of many polysaccharides, therefor has low permeability. Salts are also molecules with low permeability.
Bacterium growth on various agar plates Introduction The purpose of this experiment is to show different agar plates inhibit or enable growth of different organisms. Some varieties of media enable the grow of a wide range of organisms such as nutrient agar. Other media are selective which means they contain specific nutrients to encourage the growth of certain organisms. This means other organisms will die due to the selective nutrients such as high concentration of salt which will cause plasmolysis. Differential organisms require different nutrients which show it is fairly easy to isolate and identify organisms by using selective media.
The more concentration of the enzyme, the more browning appears. Catechol oxidase is found in cell cytoplasm, their function in plants are to "help protect damaged plants bacterial and fungal disease." The objective of this experiment is to test the presences of catechol oxidase in various fruits and vegetables. Our group hypothesis states that, If catechol oxidase is present in the selected extracts, the null hypothesis is that catechol oxidase is not present in the selected extracts. Next, the prediction would be, if catechol oxidase doesn't differ with other enzyme sources, then the rates will
When testing one organism, for example Mycobacterium tuberculosis, the dilution is prepared in agar slopes but at this time it is necessary to prepare another identical set to be inoculated with the organism which is control. To make dilution a small volume of water is used and then the dilute is added to agar that melted and then cooled to 60degrees C. If chocolate agar is required blood is added, and the medium must be heated before addition of the antibiotic. Petri dishes with 90mm diameter are convenient to be used and one ml of the desired drug dilutions is added to 19ml of the broth. Agar dilution factor must be allowed in the first calculation as follows: • The final volume of the medium in the plate equals 20ml • Top concentration of the antibiotic equals 64mgL • Drug total amount equals 1280 microgram is added to 1ml water • 2mls of 1280microgram per ml is required to start the dilution equals 2560micrograms in 2mls. • 1.28mls of 2000micrograms per ml ± 0.72ml of water.
Nutrients are chemicals elements that plants and animals require for growth. Macro nutrients nitrogen, phosphorus, and potassium are found in artificial fertilizers. My neighbor would be affecting the nutrient cycle because the fertilizer is extracting nutrients from the soil and leaving them to be placed in the aquatic environment. This affects the Nutrient Cycle. When these nutrients are taken away this can lead to the soil lacking the micro nutrients that are needed.
The variables measured are nitrate, nitrite, total phosphorus, ammonia and transparency. Harrison also states that river and Lake Eutrophication is a problem across most of Europe because of excessive nitrogen and phosphorus levels from agricultural, industrial and domestic effluents. Harrison states that natural organic matter is present in most natural eco systems. According to Harrison Eutrophication is the excessive production of algae and higher plants through the enrichment of waters by nitrogen and phosphorus. Phosphorus in the form of phosphate is the limiting nutrient as the amount of biologically available phosphorus is small in relation to the amount needed for algal growth.
Mannitol high salt testing is done in order to determine if the bacteria is salt tolerant and can ferment mannitol. Catalase activity test establishes whether the bacterium produces the enzyme catalase. The eosin methylene blue test or EMB, inhibits the growth of gram positive bacteria and tests whether or not gram negative bacteria can ferment lactose. Lactose fermentation testing is done to see if the bacterium is capable of fermenting sugar by testing for acid and gas production. These are the possible tests that are needed in order to identify unknown