Fish Transportation Research Paper

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WATER QUALITY VARIATION WHILE TRANSPORTING
HYPSELOBARBUS KURALI ANESTHETIZED WITH
2- PHENOXY ETHANOL
Sherly Williams E1 and Vishnu Nair M S2*

1. Environmental Sciences, Aquaculture and Fish Biotechnology Lab, P G and Research Department of Zoology, Fatima Mata National College, Kollam, Kerala, India.
2. Mariculture Division, CMFRI, Kochi.
*Corresponding: msvishnunair@gmail.com
Key words: Anesthesia, Hypselobarbus kurali, transportation, 2- phenoxyethanol, aquaculture.
Abstract:
Transportation of fishes from study site to the laboratory needs lot of care as the fish is very sensitive to even minor changes in the surroundings. The prime response from the fishes was random running and hitting its head and body against the medium in which …show more content…

at 20oC (Merk and company, 1989). It is used as a topical anesthetic. It is a mild toxin and may cause some irritation to the skin, therefore any contact with the eyes should be avoided (Bell, 1987). Based on human toxicology data, it may also cause liver and kidney damage (Summerfelt and Smith, 1990).Anesthetic trials were performed. Four levels of anesthetics were evaluated which includes 300 µl/l, 400 µl/l , 500 µl/l and 600 µl/l with four repeats. Healthy fishes from the wild caught group were selected for transportation. Each level of anesthetics were measured out into a 50 ml reagent bottle, mixed with 30 ml of water and stirred to disperse the chemical before adding to the anesthesia inducing tub. Observation on 10 fishes were made at each level. The stages of anesthetization were differentiated as induction, maintenance and recovery (Sajan et al, 2012). The efficiency of the anesthetics were assessed by considering three stages of induction (I1, I2, I3) and three stages of recovery (R1, R2, R3) in Hypselobarbus Kurali. An induction time of 180 sec or less and complete recovery with in 300 sec suggested by Marking and Meyer 1985 and Trzebiaoto wski, 1996 was employed to assess the induction and recovery stage in the Hypselobarbus Kurali. Dosages of anesthesia adopted for various teleosts (Weber et al, 2009) were adopted as the base information. Both treatment and recovery water were taken from the natural ecosystem itself, where the fishes lived. When the fishes reached the stage three of aesthesia (I3), it was immediately transferred to the recovery tub for recording the recovery stage (R1, R2 and R3). The induction and recovery instance for each concentration was measured by using an electronic stop watch. Experiments were repeated four times to verify the findings. The recovered fishes were

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