Evaluate The Effect Of Extraction Solvents Lab Report
EFFECT OF EXTRACTION SOLVENTS ON THE PHYTOCONSTITUENTS OF DIFFERENT PARTS OF WEDELIA TRILOBATA L. - A TROPICAL PERENNIAL MEDICINAL HERB
ABIN ANTONY1 AND SATHEESH GEORGE2*
1Indian Institute of Science Education and Research (IISER), Mohali, Punjab, INDIA
2Centre for Post Graduate Studies and Research in Botany, St. Josephs College (Autonomous), Devagiri, Kozhikode, Kerala, INDIA.
*corresponding author (george.satheesh@gmail.com)
ABSTRACT
The objective of the present study was to evaluate the effect of extraction solvents such as n-Hexane, Petroleum ether, Chloroform, Methanol and aqueous Methanol (Methanol: Water, 50:50 v/v) on phytochemicals of different parts like leaf, stem and flower of Wedelia trilobata using chromatographic studies. The chemical pattern of different extracts was compared using Thin Layer Chromatography (TLC). Variations were observed in terms of number of bands and band intensity which indicate the qualitative and
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4g of each sample was taken in a RB flask with 100mL of solvent and boiled for 3 hours. These extracts were filtered using Whatman No.1 filter paper and concentrated to 20mL on a water-bath. These extracts were kept in amber bottles and preserved in refrigerator.
Thin Layer Chromatography (TLC) studies
Thin Layer Chromatography (TLC) profiling of different extracts of leaf, stem and flower of Wedelia trilobata was carried out on pre-coated silica gel plates (60 F254 Merck) using toluene, ethyl acetate and methanol as mobile phase in the ratio 7:3:1. Different solvent extracts of same plant part were spotted on a single plate as thin bands. The developed plates were observed under visible spectrum, UV 254nm and 366nm and observations were recorded. Rf values of each compounds visible as different bands were calculated using the formula -
RESULTS AND
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