The solution was stirred at room temperature for 8h. The solvent was blown out with nitrogen. The residue was added to 1 ml of water containing 0.1% TFA and purified on RP-HPLC. Massspec of the final product clearly indicates presence of RB modified on PEI by series of peaks matching different polymer compositions (see Fig. 6).
4 Procedure Preparation of Test Solution The sample was prepared by adding 100 mg of powdered in 10 mL of methanol, soaked for 15 min. Centrifugation was done and the filtrates or supernatants were used as the sample solution. Out of this filtrate 50 μL of samples were dissolved in 1 mL of toluene and were used as the sample solution. Preparation of the standard solutions Wedelolactone, Ecliptaalbasaponin-I and Ecliptaalbasaponin-II were used as marker compounds; these markers were dissolved in methanol at a concentration of 1 mg/ml. The extracts of Bhrungaraj prepared were shaken and sonicated in methanol at a concentration of 10 mg/ml.
All standards, samples and solvents were filtered using filtered using 0.45 µm Sartorius Stedim, cellulose nitrate filter paper prior to HPLC-PAD analysis. The HPLC-PAD system consisted of a PAD detector, Perkin Elmer pump and ALS, CarbopakPA1 (4 × 250 mm) and CarbopakPA1 Guard Column (21.7 °C). The PAD detection range was set at 300 K (E1: 0.05 V, E2: 0.76 V, E3: -0.20 V). The injection volume used for analysis was 50 µL and the analysis time for each sample and standard was set at 30 minutes. The solvent system used for analysis was 10 mM NaOAc/ 150 mM NaOH and the kestoses were eluted at a flow rate of 1 mL /min.
EXPERIMENTAL PROCEDURE The specimen Al 2024 which was consist of 3.8-4.9% Cu, 1.2-1.8% Mg, 0.3-0.9% Mn, and Fe, Cr, Zn, Ti in a little amount had been inserted into a furnace set at 500oC approximately 50 minutes for a solution treatment before the lab. Its height was 7 mm and width was 25 mm. At the beginning of the lab the specimen was removed from the furnace using tongs and quenched in water. Then, the specimen was put into the oil at 190oC for 6 min. While waiting, another specimen Al 2024 at room temperature was used to measure Rockwell hardness.
Agitation speed was maintained at 350 rpm which helps in complete removal of methylene chloride. Microspheres were then collected, filtered, washed three times with distilled water and stored under reduced pressure, overnight in a desiccator. Additionally, optimization of the process was done by selecting suitable stirring device element i.e. magnetic stirrer vs mechanical stirrer in order to improve the shape and yield of microspheres. A paired t- test was applied for final selection of the stirring element based on 95% confidence
Spiked blend was assayed and used for accuracy studies. To determine accuracy, 200 mg BB and 800 mg drug were mixed properly and finally 1000 mg SB were prepared. 100 mg were then collected and tested under 80% accuracy parameter, subsequentely 100% and 120% accuracy levels were also determined. Stress degradation studies of bulk drug The forced degradation studies were carried out on bulk drug substance in order to prove the stability-indicating property and selectivity of the developed method. The degradation was carried out under acid, base and neutral hydrolytic, Oxidative and Thermolytic Acid treatment 5 ml working standard solution of Boceprevir (10000 µg/ml) was mixed with 5 ml of 1N methanolic hydrochloric acid (HCl) and 40 ml of methanol.
Milk powder brand: A or B (categorical data) 2. Temperature: 70 or 90 degrees Celsius (continuous data) 3. Mixing time: 3 or 5 minutes (continuous data) Measuring the Response To measure the residue of milk powder remaining in the experiment, I used a consistent weight of milk powder of 7 grams (in the same cup of size 1/16th) and mixed with 250ml of distilled water and after mixing, filter the residue and dry it in preheated oven at 80 degrees for 15 minutes and then measure the dry weight of the residue with an electronic scale. Designing the Experiment For this experiment, I decided to construct a 23 factorial design. I chose number of blocks to be one as I am going to finish the experiment in less than one day.
Formulation of topical gel containing plant extract  20 g gel was prepared by simple dispersion method. Different concentrations of Carbapol 934 were used to prepare the formulation. Required amount of Carbapol and adequate amount of purified water was taken in a beaker. It was mixed to get a uniform mixture using magnetic stirrer and kept overnight for swelling. 1 ml of the extract and Piperine was dissolved in ethanol and added to the polymer solution along with propylene glycol and methyl paraban with gentle stirring.
The liquid solution can be either aqueous based or solvent based. In this formulation the binding solution is PVP solution is made. Step 3: Screening the damp mass into granules. Step 4: Drying the granules (in hot air oven at 600c for one hour). Step 5: Dry screening: After the granules are dried, pass through a screen of smaller size than the one used for thewet mass to select granules of uniform size to allow even fill in the diecavity.