Introduction Wheat grain is a staple food being consumed worldwide, playing an important role in human nutrition. The main component in wheat flour is starch, constituting an average of 70% of its weight. The molecular structure of starch is made up of 2 polymers, amylose and amylopectin (Shewry, 2009), and it is these 2 molecules that makes up the backbone and branching points of the starch granule. In the process of bread making, these starch granules swell and gelatinized to due to the increase uptake of water (Yasunaga, Bushuk and Irvine, 1968). The other main component found in wheat grain is gluten, which can be used in a variety of applications ranging from food such as bread to biomedical purposes (Lagrain et al., 2010; Reddy …show more content…
α-Amylases are typically found in trace amounts in wheat flour (Sahlström and Bråthen, 1997; Goesaert et al., 2009), and in the bread industry, exogenous addition of α-amylases (derived from A. niger) increases the yield of fermentable, reducing sugars and Maillard reaction products, which affects the flavour and crust colour of the loaf (Danehy, 1986; Namiki, 1988). Yeast (S. cerevisiae) is one of the main ingredients in bread, with the major role of fermenting simple sugars, such as glucose and maltose produced from starch, thus allowing yeast to produce more carbon dioxide gas and ethanol. It is the result of the fermentation that produces a leavened loaf of bread (Beck, Jekle and Becker, 2011). This experiment aims to study the effects of exogenous addition of thermostable α-amylase on dough and bread …show more content…
Dough elasticity & extensibility Between the control and amylase dough, there were significant differences in elasticity and extensibility, where the control dough demonstrated higher resistance to stretching (higher tenacity), returning to original shape more readily (higher elasticity) and breaks apart when elongated (low extensibility) as compared to the loaf with amylase which demonstrated the opposite where it was able to stretch easily (lower tenacity), does not return to original shape (low elasticity) and elongates without breaking (high extensibility). Loaf Weight and Volume A comparison between the two loaves also showed no discernible distinctions between its weight before and after proofing (Table 1), with a 1.12% increase in control and 0.032% decrease in loaf with amylase present. However, the final loaf volume presented a significant difference between the two (Figure 1). It was found that the loaf with amylase was bigger, 13% in height, 10% in width and 24% in frontal area, than the control
The wheat was grounded into flour which was used to make bread. A
Two women are the most important in a grown man’s life, his wife and his mother. Adam Gopnik, New York University, Institute of Fine Arts graduate and a long time writer for The New Yorker explores his relationship to these women in his article “Bread and Women” (AdamGopnik.com). Gopnik describes how his sojourn into bread baking uncovered insights about his mother and spouse. He utilizes allusions, epithets, and dialogue to portray his wife and mother as important individuals who are unique and interesting in their own rights. Gopnik uses allusions to ancient buildings and famous figures to clarify the complex personalities of his beloved muses.
But, basically all bread processing rely on these 4 key steps which are mixing, fermenting, baking and cooling. Bread is made with three basic ingredients: grain, water, and bakers ' yeast, while wheat flour, water, salt, sugar accounts for more than ninety five percent of bread mass, remaining three to four percent ingredients are the key ingredients that convert these humble daily used food ingredients in to appetizing bread. The method start with the sifted flour is poured into an industrial mixer. Then, Temperature-controlled water Bread is piped into the mixer to produce a mixture called gluten and the function of it to give elasticity to the bread.
Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.
The iodine test determines the presence of starch in biological materials. It is predicted that, if starch is not present, the solution with iodine remains yellow. However, if starch is present the solution with iodine becomes a blue-black colour. Plants have starch as the storage polysaccharide (glucose units held together by glycosidic bonds) while animals have the equivalent of glycogen. In this experiment, the dark blue colour is visible because of the helical amylose and amylopectin reacting with iodine (Travers et al., 2002).
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
I predicted that the control would have a higher alcohol content than the experimental since beta and alpha amylase are working together. Since only Alpha-Amylase worked in the experimental, there was probably bigger carbohydrates present in the flask, therefore, there was a lower alcohol percentage since yeast can’t digest bigger sugars. b. My results also matched my prediction regarding mean reducing carbohydrate levels during the mashing process between the control and the experimental. My prediction stated that there would be less reducing carbohydrate ends in the experimental, which was proven in the data table.
Type 2 diabetes rates have been rising in Canada and around the world and are due to excessive weight gain, obesity and physical inactivity. It is known as a disease where the pancreas does not produce enough insulin in the body, therefore resulting in glucose building in the blood whereas it should be used for energy. According to the World Health Organization, the number of people with diabetes in Canada will increase by 75% over the next 30 years from 2 million to 3.5 million patients. Moreover, diabetes is a rising concern as it leads to diseases such as heart, and kidney disease, stroke, infections and low levels of blood sugar. This paper explores the issues of diabetes and solutions to prevent it by increasing physical
1.1 Abstract The purpose of quantitative analysis of protein using a spectrophotometer is to measure the concentration of proteins in a given sample. The experiment is conducted by laboratory method (Biuret Test) and using spectrophotometer to analyze the absorbance of reactants at 540 nm, hence determining the concentration of the proteins in a given sample. The purpose of stopped enzyme assay to study B-galactosidase is to determine the effect of temperature and concentrations of substrate on enzyme activity.
What is the effect of temperatures 10°C , 20°C, 40°C, 60°C and 70°C ± 1/°C on yeast fermentation when baking bread? ii. Aim: The focal aim of this experiment is to investigate the effect that temperature has on the growth and respiration of yeast (Saccharomyces cerevisiae) fermentation. iii.
Panera Bread Company What is Panera Bread? They serve quality food with speedy service but not too fast like McDonalds or other fast food restaurants nor as expensive and slow as full dine in restaurants (i.e. Chili’s or Applebee’s). “Panera Bread offers freshly baked artisan bread to neighborhoods in cities throughout the country. As of September 27th, 2016, Panera Bread has 2,024 baker-cafes in 46 states” (panerabread.com).
Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to
Effect of Yeast on Temperature on Hydrogen Peroxide Solution in Water Khalid Al Sabeeh Ms. Dobrin 11-G Chemistry HL Jan 5, 2015 Abstract: Within this lab yeast was added to hydrogen peroxide solution in water. Temperature was the factor to be tested. In all trials, the initial and final, when yeast was added temperatures increased by 10˚C respectfully per trial.
We use it in a myriad of foods and recipes and we use it as a source of our energy for the day. Used for generations in thousands of years, we eat bread to come together in our lives. “Americans still consumed about 30 percent of their calories in the form of bread.” (Morano 1) So I leave you with this: I’ve never seen a food that brought people together like bread, we eat it in almost everything, and that’s why I picked this topic; bread is so fascinating, it is in a myriad of things in our life, and nothing can ever truly compare to a perfect freshly baked loaf of
Day Five you do not see any changes on the white bread where as the whole wheat bread has started to form small amounts of the mold but not yet extremely visible. Day Seven you start to see small amounts of mold growing on the white bread but not yet enough to throw it away, where as the whole wheat has already gotten bread mold but at this point those small amounts are increasing there size and starting to spread and take over the surrounding area. On day Twelve you see that both breads have both gotten a nice amount of mold covering but the only difference is that the white bread mold has not spread on a larger area but has clumped together and started changing its colour from a green to a yellow-brown colour, where as the whole wheat bread has kept the green colour and it has spread more widely around the area of the