Top agar was dispensed into 50ml test tubes and then autoclaved for 15 minutes at 121℃ as well. Stored the top agar at -5℃ refrigerator when it cooled down. Preheated top agar for 20 minutes to liquefy before use. The optimal dispense temperature for top agar was 45℃. 3.3.4 Preparation of CaCl2 solution The molecular weight of CaCl2 was 111 g/mole.
Method A more detailed method is provided in the 280.201 Industrial Microbiology lab manual. However, the basic steps of carrying out this experiment are as follows: • Five agar plates are provided including: 1. Nutrient agar 2. Mannitol Salt agar 3. Eosin-methylene blue agar 4.
.4 Agar Disk Diffusion The antimicrobial tests were carried out according to disc diffusion tests (Lennette et al., 1985, Kim et al., 1995). Cells were streaked on MHA agar plate to obtain colonies at 37°C for 16 hrs after which they were resuspended in sterile saline solution to give an O.D of 0.1 at 600nm. On solidified 2% MHA plate, the bacterial culture is swabbed and sterile disc impression was made, 10 µl plant extracts was loaded on the agar plate. After 10 minutes the plate is incubated at 370C The diameter of the clear zone was measured. Fig 9: Agar disk diffusion 5.5 Time Kill Assay The basic concept of the Time-Kill Kinetic study is establishment of the rate at which a microorganism is killed by a product as a function of survival
Your Agar plate should now be sterilized, the process of sterilization should be finished. Procedure of the experiment: Prepare all the test tubes and place them in their respective test-tube holders. Put 10 millimeter of the 0.9 % saline solution in one test tube. Put 10 millimeter of the chicken broth in the other test tube. Put 1 capsule of the bioflorin into each of the test tubes and tap them until the capsule dissolves.
(REFERENCE) Chocolate Agar- Used for capsule demonstration. When subculture are to be done on this medium and incubated at 37oC in a candle jar with raised pCO2 mucoid colonies were obtained after 24 hours (Perfect JR and Cox GM, 2005). Colony morphology C. gattii and C. neoformans grow readily on SDA. Colonies
Bacteria that can directly convert sugars to acetic acid in an anaerobic fermentation include Clostridium and Acetobacterium but they can not tolerate the acetic acid concentrations greater than a few percent. The product made from these bacteria should be focused while oxidative fermentation by Acetobacter can produce up to 20% acetic acid. Fast Technique: R. and R. schutzenbachi curum is used in this process. Acetic bacteria forms a thin silky film. Wine, grapes, grape fruit, malt, corn syrups, can be used as raw material.
22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot plate 2. pH of the solution was adjusted to 7.0 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Lauryl Sulphate Broth
citri (Xcc), was provided by the Bacterial Citrus Canker Collection of National Institute of Genetic Engineering and Biotechnology (NIGEB). The bacterium was cultured on YPGA (3 g/l yeast extract, 5 g/l peptone, 7 g/l glucose solidified with 15 g/l agar) at 28 °C for 48 h. A single colony was subcultured in YP medium (3 g/l yeast extract and 5 g/l peptone) on a rotary shaker at 180 rpm at 28 °C till OD600 = 0.3, i.e., 5 × 108 cfu/ml. To prepare the inoculum, the culture was centrifuged for 10 min at 5000 ×g and the pellet was suspended with dH2O to reach a concentration of 1 × 105 cfu/ml. For inoculation, host plant leaves were surface-sterilized with ethanol and the inoculum (500 µl of 1 × 105 cfu/ml) was injected into the parenchymal space on the abaxial leaf side. Control leaves were treated similarly
Apgar score was recorded with in 5 min of birth for all newborns by single examiner. After obtaining informed consent from parent, 2 to 3 ml of cord blood was collected immediately after birth in the labor room. Newborns’ Blood groups were detected by Blood group typing antisera from Ortho diagnostics. Cord blood hemoglobin estimation was performed by Drabkin’s reagent supplied by Nice Company and CS-Albumin estimation by BCG dye binding method on Shimadzu-UV17000- spectrophotometer. Record of mother’s Serum Albumin and hemoglobin levels were obtained from the prenatal checkup file.