Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known FD&C and for the three Kool-Aid samples. The retention factor for each dye was calculated.
7)Use toothpicks to transfer the dye from the skittles onto the coffee filter strip. 8)Measure about 1/4 inch from the top of the coffee filter strip and tape pencils to the top. 9)Place each coffee filter strip over a cup with the pencil holding and balancing it at the top. 10)Fill cup with water so the water is slightly touching the coffee filter strip. 11) Once the dye has reached the near top of your cut coffee filter (1/4 inch) take them out and dry them.
The unusual letter formation made it possible to conclude that Talia Zagon had written the note. The paper analysis test also confirmed our thinking in which the known paper from Talia Zagon matched with the unknown paper that the note was written on at the crime scene. Both papers have a woven fiber structure, show small glowing fabrics under UV light, and are semi-thick in size. These characteristics were able to help us identify that Talia Zagon had used the paper left in the crime scene. From the ink test, we also could conclude that Talia Zagon is the murderer because her Rf values for her ink were the most similar to the Rf values of the unknown ink.
The anthraquinone dye experiment has the purpose to identify the anthraquinone dyes from unknown mixture by using thin layer chromatography (TLC) of the unknown fraction. An anthraquinone is an aromatic organic compound obtained by the oxidation of anthracene. To separate the compounds in the mixture, column chromatography and thin layer chromatography uses portioning of a sample between a stationary solid phase and a liquid mobile phase. As the stationary phase, they use either silica gel or alumina, and organic solvents as the mobile phase. In order to accomplish the experiment, an unknown which is a solution of at least two anthraquinone dyes will be used.
Summary Determining the concentration of a liquid can be a tricky process involving complex procedures if it were not for science’s ability to test a substance’s absorbency through spectrophotometry. The experiment was carried out to discover the concentration of Red Dye #40 in several common soft drinks. The samples of the dye were diluted, and tested using a spectrophotometer. The absorbencies of these samples were then recorded, and a standard line curve with the concentration equation and R2 value was created with these results. Using the absorbencies of the dye samples, the concentrations of the soda samples were determined using the slope equation provided by the graphing software.
MM 3320 : Report Mass Spectrometry Submitted by Velu K R NA12B033 Introduction Mass Spectroscopy is an instrumental method for identifying the chemical constitution of a substance by means of the separation of gaseous ions according to their differing mass and charge. This method helps identify the amount and type of chemicals present
Using forceps, 5 leaf discs each are put into 10 syringes. They are pushed to the bottom of the syringe with the forceps. 5. 400ml of distilled water along with 8g of sodium bicarbonate are put in a large beaker to prepare a solution. They are stirred to mix them and prepare a solution.
This helped in nabbing the criminal. Therefore, scientific examination of crime scene is that the primary, essential and most important step towards investigation of crime . The scene of crime and laboratory findings was confirming. additionally to those, post mortem report additionally discovered intra blood vessel injection marks on the hand of all deceased. of these evidences cumulatively counsel that the perpetrator should be medically trained person.
Forensic science uses many areas of the known science and related them together to create this developed science called forensics. forensic use different part of science such as: 1- Chemistry: Chromatography: which is a method that is used to separate mixture of components between two different phases, and there are many types of chromatography. Spectroscopic analysis: is the investigation of the cooperation between matter and electromagnetic radiation, and this term is used in degraded
The investigators started with photographs and videotaped from both the ground and air. These picture and video along with the sketches of the areas would make it easier to recreate the crime scene if required by the prosecution. The investigators started at the parking lot and worked forward until they reached the victims body, which had already been examined by the Chief Medical Examiner. The investigator made note of the position of the body and based on the position they judge that she was dragged by her feet and ankles. At this point the investigator, check the body where they found multiple stab wounds to the neck and abdominal regions and once in the upper back.
Upon seizing the evidence, the officer should perform a Duquenois-Levine color test on the suspected plant material. The Duquenois-Levine color test is in a plastic bag resembling container with three tubes of liquid inside. The color test reagent kit is easier to do since it is packaged with the reagents is sealed in the glass tubes for a single test for field purpose. Once a small amount of the marijuana was in the container next I broke the reagent tubes. After all the tubes had been broken I began shaking the container till the color of the liquid started to change.
We had to go back into our notes and see what matched to what. Materials I used. Were a petri dish, scapula, liquids, burner, C-clamp, tongs, gas to start the flame, eight different types of powders, and the most importa martial was our goggles for safety. The petri dish was used to put the powder in for testing. The scapula was used for scooping up the powders.
Ways to collect the prints is through photographs or type. Submit the intentionally recorded prints of everyone who may have handled the evidence, including the suspects and victims to help narrow down or eliminate to find the right person (FBI Laboratory Publications, 2013). Tying in the drug processing and fingerprinting, you can search for a latent print on a drug packaging materials. The drug item should be separated from the original packaging when it is reasonable to do
The purpose of the lab is to find out the suspect who stole the teacher’s grade book by taking a series of test, evidence, and analysis. We’re testing the foot powder for evidence and have to figure out the ingredients in the foot powder. The series of the test contains five test. The test to start with is a visual examination. We’re supposed to record the color and texture.
In science class, we did an experiment to compare inks and their compositions. This was intended for us to learn about chromatography; the separation of a mixture through a medium (the chromatography paper) in which the components move at different rates and create different patterns. We chose to explore this topic by using four different colored markers, placing a dot of each color onto one piece of chromatography paper, and observing while the water in the beaker travels up the paper and separates the dyes. Before we started, we made a hypothesis. Mine was if paper chromatography is done on the ink in the green, red, brown, and black marker, then the green will spread out into different shades of blue and green making a pointed oval shape.