Saccharomyces Cerevisiae (yeast)is a single cell eukaryotic organism that is a fungi. It digests food to obtain energy for growth and gets it mostly from sugars like sucrose, fructose and glucose and maltose. When sugar is present, yeast conducts fermentation to produce alcohol and carbon dioxide by creating a chemical energy.In yeast, high sugar concentrations and high specific growth rates trigger alcoholic fermentation, even under fully aerobic conditions. It is commonly used to leaven bread, mold blue cheese to make it ripe, ferment alcohol, and is used in the molds that produce antibiotics for veterinary and medical use. In bread baking the sugars from the flour or from the added sugar are fermented by the yeast, because the dough is
Medium and culture conditions Cells were grown in a 100-mL flask containing 25 mL into minimal medium supplemented with Eugenol for 2, 4 and 6 days incubation at 37 °C. The following Table 3. represents the composition of the modified minimal media used for biotransformation of eugenol (Muheim and Lerch, 1999). The pH of the medium was adjusted between 7.0 to 7.25 and autoclaved to obtain sterilized media for further
coli (Table 1) by using the well agar diffusion method. The multidrug resistant pathogens were initially propagated at 37 °C in nutrient broth. The overnight grown cultures were then again sub-cultured into nutrient broth media for 2 h till 0.01 OD. Subsequently, 100 μL of each culture was then spread uniformly onto nutrient agar plates. Wells of 6 mm diameter were made on agar plates using an agar well borer.
After which the digestions were examined by gel electrophoresis. The samples were run on a 50 mL 0.9% (w/v) agarose gel in 1X TAE buffer at 100 V until the leading track dye traveled 2/3 the distance of the gel. The gel was then soaked in GelRed for 20 minutes and examined under UV light. To prepare the digestions 10 μL of each digestion was mixed with 2 μL of 6X track dye in a micro centrifuge tube. 12 μL of 1 kb DNA ladder and each digestion was run on the
Place melted agar solution and pre-warmed 2x culture medium in an ice bucket filled with hot tap water (42 °C). Also place a 50 ml conical tube in a tube holder in the ice bucket with hot water. Transfer bucket to cell culture hood for subsequent steps. 3. For the bottom layer of agar, you will need 1.5 ml of a mix of agar and medium per well of a 6-well
And , it was washed .About 120g of dried leaves were obtained from fresh leaves weighing about 24g .And, they were powdered and extracted with 100 % Ethanol for about 48 hours. After extraction, the Ethanol extract was filtered by mesh cloth. . 2-Preparation of nutrient agar plates: Cup –plate method was used for screening the antibacterial activity of pure and dried 100 % ethanol extract .A commercial sample of amoxicillin was used as a standard and nutrient agar was used as culture medium. The natural agar consist of Yeast Extract, Tryptone , Lactose , Manniiol, Sodium Chloride , Dip otassium Hydrogen Phosphate ,Gelatin and Agar .Then , in a conical flask 14g of nutrient agar was mixed into 500 ml of distilled water and , The mixture was stirred and dissolved until most of the agar dissolve.
In a separate beaker, 10-3 M of synthesized SB was dissolved in 10 ml DMF. The two solutions were mixed together under stirring and resulting yellow precipitate solution was transferred to a sonochemical bath. After 60 minutes of sonochemical treatment, the resulting CdS precipitate was collected, filtered, washed with double distilled water and absolute ethanol several times to remove the unreacted chemicals, and finally dried in an oven at 80oC for 5hours. Similar procedure was adopted to synthesize uncapped CdS
HFCS is prepared by the chemical and enzymatic hydrolysis of corn starch comprising amylose and amylopectin to corn syrup covering typically glucose charted by the isomerization of the glucose in corn syrup to fructose to produce HFCS. HFCS is baptized iso-glucose in England and glucose-fructose in Canada, and was leading familiarized to the nutrition and infusion manufacturing in the getting on 1965 and 1975s to progress constancy and functionality of numerous foods and infusions. Physical and Chemical properties: Dry Substance % 76~77% PH 3.3~4.3 Ash % Trace SO2,ppm <
The tube was placed back in incubation for 96 more hours to observe any more positives. 2.10 Catalase Test A trypticase soy agar plate was used and after incubation, four drops of 3% Hydrogen Peroxide was added to the plate to flow over the bacterial growth. A presence of bubbling was observed. 2.11 Starch Hydrolysis A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids.
32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate. 50 μL of these dilution solutions were separated on the TLC plate coated with SNISG. The plate was developed with petroleum ether: ethyl acetate (4:1) and the movement of solvent was usually controlled at 1 cm from the upper edge. After completion, the plate was dried until no solvent smell remained. It was sprayed with an ethanol solution containing 10% sulfuric acid, and heated at an infra-red drier until obvious color came up, as shown in Fig.2 (B.ab).