These results accept the hypothesis: if yeast can metabolize, then the bromothymol blue solution should turn yellow from the production of carbon dioxide. Only the bromothymol blue solution with yeast turned yellow, suggesting that the yeast caused the color change. The yeast consumed sugar, produced
While the absolute value of slope of the graph for the solution containing only 0.5 mL mitochondrial suspension was 4 x 10-4, the slope of the graph for the solution containing 0.5 mL of mitochondrial suspension, 0.5 mL of 100 mM succinate, and 0.5 mL of 100 mM malonate was 7 x 10-4. Although this change is not large, it does demonstrate that the addition of TCA cycle intermediates has an impact on reaction rate. The decrease in the rate of reaction of the sample containing 0.5 mL of mitochondrial suspension, 0.5 mL of 100 mM succinate, and 0.5 mL of 100 mM malonate as compared to the sample with only 0.5 mL of mitochondrial suspension and 0.5 mL of 100 mM succinate shows that the addition of malonate inhibits the reduction of
The T table value is 1.0371. This number’s close proximity to 0 concludes that there is no significant difference in the number of coacervates. o we reject the alternative hypothesis, and accept the null. The strengths of this experiment are that there was the ability to test size and number in the same process.
1% glucose, 1% maltose and 1% lactose all progressively get positive results by changing colours to reddish brown at the end of this experiment. In this case the aldehyde functional group that is present in the products (monosaccharides and some disaccharides) in this reaction is able to reduce copper in the presence of alkali and this produces colour changes while converting to an aldose sugar. Honey is made of fructose and glucose which instantly turned brown after the test-tube was placed in the boiling water because of its active aldehyde and carbonyl group. The copper (II) sulphate present in the Benedict’s solution reacts with electrons from the aldehyde group which results in a redox reaction to from cuprous oxide, a red brown precipitate that seen in all of the above mentioned solutions (Hill, 1982). Beer also gave positive results because it contains aldehydes and ketones (i.e. acetone, trans-2-butenal, furfual) during its beer production process where the sugars are converted through fermentation (Hill, 1982).
Therefore, liquid-liquid and acid-base extraction techniques were successfully performed to separate the components of the Excedrin tablet. According to the TLC analysis results, the compounds (aspirin, acetaminophen, and caffeine) were successfully isolated from the analgesic (Excedrin tablet). In figure 1, the separation of the compound in the TLC analysis correlates with the TLC analysis in figure 2. Furthermore, Rf index calculations of the TLC analysis demonstrated that the compounds (aspirin, acetaminophen, and caffeine) were separated. The Rf calculations of aspirin in table 1 shows an Rf value of .491; however, in table 2 the Rf value of aspirin was calculated to be .784. This Rf value is the higher among the other compounds because the Rf values decreases from aspirin to caffeine; therefore, this suggests that the
The Effect of Sugar Concentration on CO2 Production by Cellular Respiration in Yeast Introduction In this lab, our main focus was to find how sugar concentration affect yeast respiration rates. This was to simulate the process of cellular respiration. Cellular respiration is the process that cells use to transfer energy from the organic molecules in food to ATP (Adenosine Tri-Phosphate). Glucose, CO2, and yeast (used as a catalyst in this experiment) are a few of the many vital components that contribute to cellular respiration.
Multiplication of yeast is caused by several factors , a nutrient poor diet and stress will suppress our immune systems and upset the balance of friendly bacteria. Antibiotics used to treat ear,nose and throat infections (tetracycline and vybramycin ) will eradicate all the friendly bacteria (acidophilus, bifidus, bulbous etc) in the colon. Yeast will feed on sugar, damp conditions and environmental moulds will all cause it to multiply.
5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).
The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.
During the first cycle of replication in meiosis, Prophase is the same but crossing over occurs along side of the nuclear membrane dissolving, chromosomes developing, and the spindle fibers forming. Crossing over is the process in which homologous chromosomes from both parents pair up and exchange DNA. Also during metaphase and anaphase homologous chromosomes are separated and pulled to opposite sides. During this second cycle of replication the cells grows through Prophase II, Metaphase II, Anaphase II, Telophase II, and its final cycle of cytokinesis which is the exact same as during mitosis. I will play a quick review of this process.
(D)- 2- Hydroxyglutarate which accumulates in cytosol. This leads to inhibitions of several enzymes dependant on alpha-ketoglutaric acid. The whole process results in hypermethylation of DNA and histones. The hypermethylation can activate oncogenes and suppress tumor suppressor genes. The company reckons that inhibition of these mutated IDH may lead to clinical benefit for those patients with cancers which are carrying mutated IDH.
The Another medium used was MAC, it is used to isolate and differentiate gram-negative organisms and it is a pink, dusty rose color. Lastly, the Citrate Slant is a green color and it was used as a differential test to examine enzymes. The media were inoculated at 37°C for 48 hours, then it was observed to determine the