Yogurt is a fermented milk product that contains the bacterial cultures such as Lactobacillus bulgaricus and Streptococcus thermophilus. All yogurt must contain at least 8.25% solids not fat. Full fat yogurt must contain not less than 3.25% milk fat, low fat yogurt not more than 2% milk fat, and non fat yogurt less than 0.5% milk. Milk is the main ingredient in yogurt. The type of milk used depends on the type of yogurt – whole milk for full fat yogurt, low fat milk for low fat yogurt, and skim milk for non fat yogurt. To adjust the composition, other dairy ingredients are allowed in yogurt such as cream to adjust the fat content, and non fat dry milk to adjust the solids content. Probiotics like Lactobacillus bulgaricus and Streptococcus …show more content…
Streptococcus thermophiles, as the name convey, looks like a chain of spheres. It is a gram-positive microbe. The cell wall is composed of N-acetylglucosamine (NAG) and N-acetylmuranic acid (NAM), which is bond by ether bonds. This particular structure allows S. thermophilus to cope with raised temperatures, which is beneficial for many industrial dairy fermentations be in need of the process of milk at higher temperatures. S. thermophilus is a facultative anaerobe, which is an organism that is having the ability of generating ATP through both aerobic respiration and fermentation. This capability is depending on the presence or absence of oxygen. Besides, S. thermophilus is shortage of genes or contain pseudogenes expressing surface protein (excluding lipoproteins); pathogenic streptococci use these surface proteins to attached to mucosal surfaces and avoid host defense mechanisms. S. thermophilus does not form spores and does not move on its own. As the species name gives away, the microbe likes warmer temperatures. Its optimum growth rate take place at the bottom of the thermophile spectrum, around 45°C. It is also capable of create energy, in the form of adenosine triphosphate (ATP), by aerobic respiration with the existence of oxygen; however, without the presence of oxygen, it still can produce ATP through fermentation. S. thermophilus lacks cytochrome, oxidase, and catalase enzymes. Although S. thermophilus is nearly linked to other pathogenic streptococci such as S. pneumoniae and S. pyogenes. S. thermophilus is classified as a non-pathogenic, alpha-hemolytic species that is part of the viridians
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
Staphylococcus epidermis produces the enzyme catalase. In the PEA Agar, a catalase test was performed which showed that the organism produced catalase. Staphylococcus epidermis is not a mannitol fermenter. Mannitol fermenting organisms grow on the Mannitol Salt Agar. The unknown organism is not a mannitol fermenter because it did not grow on the Mannitol Salt Agar.
Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Tests: Lactose fermentation: Fermentation makes energy available for use by microorganisms by anaerobic breakdown of carbohydrates. The product can either be an acid or gas. When it is positive, the broth will turn from red to yellow and if gas is present a bubble is formed.
Inflammation is the main type of innate immunity our body uses against A. schmiddy. Inflammation begins by activating acute-phase proteins, and soon after vasodilation occurs, followed by redness, swelling, pain, and heat. Once vasodilation occurs, histamine and kinins are released and blood vessels permeability is increased, causing white blood cells to report to the infected area. One problem associated with inflammation is that the capsule of A. schmiddy is an important virulence factor, which helps the bacteria to resists phagocytosis. Because the phagocytes cannot destroy the bacteria cells, infection further continues and necrotic tissue forms.
A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids. The smoke of the Iodine stained the plate to display the presence or absence of a halo around the bacteria 2.12 Lipid Hydrolysis This test was done by making a single line streak inoculation on a tributyrin agar plate and allowing incubation. After the incubation period, the plate was observed for the presence or absence of a halo around the bacteria.
According to "Got Milk ? You Don 't Need It," Mark Bittman (2012) suggests that "propaganda" is being stimulate by the American government despite the fact many people have sensitivity toward milk, and there is other alternative to milk. The writer focuses on the reasons why people do not have to drink milk. First, he is explaining that milk allergy is one of the most common allergies in United of States, and that many people are suffering from stomach problems. Moreover, he provides his experience with cutting dairy output to cure his stomach issues
It is utilized to thicken frozen yogurt, cheddar, and
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
Only a motile bacteria could travel that far from the center of inoculation. Furthermore, it confirmed that P. vulgaris is a facultative
Staphylococcus Aureus belongs to the extremely common bacteria of microflora of the skin and mucous membranes of the humans. These pathogens cause many infections, including superficial and deep purulent infections, poisoning, urinary tract infection etc. In the US, staphylococcus bacteria are supposed to be the leading cause of sepsis, postoperative wound and prosthesis infections. In addition, staphylococcus belongs to one of the leading causes of bacterial food poisoning. Staphylococcus Aureus is one of the most dangerous human pathogen.
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
Our bodies do not work as hard to digest milk if they were to digest other foods. If a person is not lactose intolerant, their body should handle the consumption of dairy just fine. However, if a person lacks the lactase enzyme, or has a small amount of it, it becomes harder to break down lactose into more easily absorbable sugars, glucose and galactose
Everyday food Abstract The article discusses the role of food as an instrument of identity and a channel of contact through cultures. This is discussed drawing from three cases of Italian food culture hybridization spanning from the early 20th century to the first decade of the 2000s: the role of Italian food in Italian-American identity as depicted in Leonardo Coviello’s work; the meeting of Southern and Northern food cultures following the Italian internal migrations in the ‘50s and ‘60s; the food practices of international migrants in the context of the global flows of people and commodities in present day Italy. In this regard, food plays an essential role in the rebuilding of a familiar context in which migrants can feel temporarily
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.