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Zebrafish Heart Regeneration Lab Report

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When it comes to a species such as Zebrafish, they have the ability to regenerate their heart up to 20% after amputation in about one month. The purpose of this experiment is to determine the importance and origin of former cardiomyocytes and how they serve a role in regeneration. A cre/lox plus tamoxifen system is used to manipulate genetic material in cardiomyocytes and determine its original lineage. A major key finding is that Zebrafish cardiac muscle cells minimally switch up their pattern of genetic expression to an earlier stage, which is seen in their destructed muscular tissue structure. During cellular division, the expression of plk1 helps increase the number of cardiomyocytes in heart regeneration and helps differentiate the cells back into mature cells. The major conclusion of the experiment is that Zebrafish heart regeneration is a result of pre-existing cardiac muscle cells, not from a distinct parent cell. Introduction
The problem being examined is not being able to know the definite source of recently developed cardiomyocytes in Zebrafish heart regeneration. The hypothesis is that previous cardiomyocytes originate from progenitor cells. It is essential to understand that …show more content…

The TEM showed the cardiac muscle structure in the untouched hearts in a Z-shape (a, b), while the regenerating heart cell arrangement began disconnecting and created lots of space between the cells (c, d). The myosin seen in the normal heart turns out to be disorganized (f), and after 7 days the space was once again present (e, f). Around the 7th day, the distortion of the cell structure increased, resembling those with BrdU. However, TUNEL proved that the cardiomyocytes appeared normal and showed no signs of apoptosis. Cells with the PH3 marker showed no signs of noticeable sarcomeric organization, while PCNA-positive cells showed the

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