Zeylanina Lab Report

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3.1.1 Specimens preparation
Healthy Leaf of S Zeylanica extracted from a living specimen of the plant were fixed in FAE (1:1:18) (formalin – 5ml + Acetic acid -5ml + 70% ethyl alcohol-90 ml) for 48-72 h. The specimens were dehydrated through a graded series of tertiary butyl alcohol (TBA) [21]. Infiltration of the specimens was carried by gradual addition of paraffin wax (melting point 58°- 60°C) until TBA solution attains super saturation. The specimens were cast into paraffin blocks. [22,23] 3.1.2 Sectioning
The paraffin embedded specimens were sectioned with the help of Rotary Microtome as shown in the fig [4]. The thickness of the sections was in the range of 10-12 µm. Dewaxing and the staining of the section can be done by a customary …show more content…

The samples were crushed and extracted with the dichloromethane before being hydrolyzed in 72% solution of sulphuric acid. The only insoluble component. Lignin, was separated and calculated. The cellulose content was measured according to the [26].The samples were crushed and extracted with the dichloromethane, then the mixture of ethanol and 95% of nitric acid was added . The cellulose corresponds to the insoluble fraction of the samples. The determination of hemicelluloses (pentosanes) was carried out according to the standard NFT 12-008.Hydrobromic acid is used to heat the samples. The hemicellulose was transformed in to the furfural. It was later extracted by distillation process and measured by …show more content…

This method involved extraction of SZF and the fibers were chopped into 10mm and kept in the hot ethanol for 6 h, which is claimed to extract more wax, more quickly than any other solvents. The resulted solution is the mixture of sugar, wax, and other alcohol –soluble substances. The solution wetted fiber is transferred to a separator funnel; chloroform was added to the extraction of wax from the substances such as fatty acids, fatty alcohols, resins, plant steroids, and mono, di, triglycerides. The purified water was added and two separate layers of chloroform and alcohol was formed. After heating the funnel, the chloroform was evolved, leaving the waxy deposit. The dried residue and SZF samples were extracted and weighed. The percentage of wax content was calculated by using the following

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