3.1.1 Specimens preparation
Healthy Leaf of S Zeylanica extracted from a living specimen of the plant were fixed in FAE (1:1:18) (formalin – 5ml + Acetic acid -5ml + 70% ethyl alcohol-90 ml) for 48-72 h. The specimens were dehydrated through a graded series of tertiary butyl alcohol (TBA) [21]. Infiltration of the specimens was carried by gradual addition of paraffin wax (melting point 58°- 60°C) until TBA solution attains super saturation. The specimens were cast into paraffin blocks. [22,23] 3.1.2 Sectioning
The paraffin embedded specimens were sectioned with the help of Rotary Microtome as shown in the fig [4]. The thickness of the sections was in the range of 10-12 µm. Dewaxing and the staining of the section can be done by a customary
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The samples were crushed and extracted with the dichloromethane before being hydrolyzed in 72% solution of sulphuric acid. The only insoluble component. Lignin, was separated and calculated. The cellulose content was measured according to the [26].The samples were crushed and extracted with the dichloromethane, then the mixture of ethanol and 95% of nitric acid was added . The cellulose corresponds to the insoluble fraction of the samples. The determination of hemicelluloses (pentosanes) was carried out according to the standard NFT 12-008.Hydrobromic acid is used to heat the samples. The hemicellulose was transformed in to the furfural. It was later extracted by distillation process and measured by …show more content…
This method involved extraction of SZF and the fibers were chopped into 10mm and kept in the hot ethanol for 6 h, which is claimed to extract more wax, more quickly than any other solvents. The resulted solution is the mixture of sugar, wax, and other alcohol –soluble substances. The solution wetted fiber is transferred to a separator funnel; chloroform was added to the extraction of wax from the substances such as fatty acids, fatty alcohols, resins, plant steroids, and mono, di, triglycerides. The purified water was added and two separate layers of chloroform and alcohol was formed. After heating the funnel, the chloroform was evolved, leaving the waxy deposit. The dried residue and SZF samples were extracted and weighed. The percentage of wax content was calculated by using the following
%% Init % clear all; close all; Fs = 4e3; Time = 40; NumSamp = Time * Fs; load Hd; x1 = 3.5*ecg(2700). ' ; % gen synth ECG signal y1 = sgolayfilt(kron(ones(1,ceil(NumSamp/2700)+1),x1),0,21); % repeat for NumSamp length and smooth n = 1:Time*Fs '; del = round(2700*rand(1)); % pick a random offset mhb = y1(n + del) '; %construct the ecg signal from some offset t = 1/
In this lab, the oxidation of a secondary alcohol was performed and analyzed. An environmentally friendly reagent, sodium hypochlorite, was used to oxidize the alcohol, and an IR spectrum was obtained in order to identify the starting compound and final product. The starting compound could have been one of four alcohols, cyclopentanol, cyclohexanol, 3-heptanol, or 2-heptanol. Since these were the only four initial compounds, the ketone obtained at the end of the experiment could only be one of four products, cyclopentanone, cyclohexanone, 3-heptanone, or 2-heptanone. In order to retrieve one of these ketones, first 1.75g of unknown D was obtained.
1. What area/aspect of this setting is the most challenging? 2. In the setting, you work in, is there a certain population of patients you see more? How does this affect you?
First of all, an alka-seltzer is an a little tablet that helps with multiple pain. For example, alka-seltzer helps with migraines, headaches, sinus headaches, muscle pain, and symptoms of pain. Alka-seltzer contains aspirin, sodium, hydrogen carbonate, citric acid, natural flavors, and artificial flavors. These are just some of the ingredients. When an alka-seltzer dissolves it releases all of the medicine.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
Prove if the material in cup 6 is a metal, metalloid, or nonmetal, by using its appearance, color, state of matter, luster, conductivity, malleability, and how it reacts with HCL. Before beginning to test on the substance we observed its appearance, state of matter, luster, and color. The substance was very shiny, solid and hard, as well as silver. Then we put on safety goggles to start testing.
Chem 51 LB Experiment 3 Report Scaffold: Bromination of Trans-Cinnamic Acid 1. The goal of this experiment was to perform a halogenation reaction through the addition of two bromides from pyridinium tribromide. This was accomplished by reacting trans-cinnamic acid with pyridinium tribromide. After the reaction took place, melting point analysis was conducted to find out the stereochemistry of the product, which could either be syn-addition, anti-addition, or syn + anti-addition. 2.
Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
In this lab there were five different stations. For the first station we had to determine an unknown mass and the percent difference. To find the unknown mass we set up the equation Fleft*dleft = Fright*dright. We then substituted in the values (26.05 N * 41cm = 34cm * x N) and solved for Fright to get (320.5g). To determine the percent difference we used the formula Abs[((Value 1 - Value 2) / average of 1 & 2) * 100], substituted the values (Abs[((320.5 - 315.8) /
In this three-week long experiment conducted in the Bio 13 Lab, we were able to analyze a single nucleotide polymorphism (SNP) in our own genomic DNA and then determine our genotype at this specific SNP. In week one, we extracted genomic DNA from our cheek cells with swabs and prepared our DNA for PCR (Polymerase Chain Reaction) that would amplify the region with the intended SNP of interest. After one week and after the PCR was run outside of the lab section, the resulting PCR product was purified and treated with restriction enzyme Ahdl in order to prepare for the final analysis of our genotypes. In the third and final week of the project, we analyzed our PCR products by means of agarose gel electrophoresis. By the conclusion of the experiment, we had completed the analysis at the SNP of interest and determined our genotypes for this SNP.
In acidic aqueous solution, a buffer is formed by the dissociation of the acid: HA ⇄ H+ + A- Hence, when acid is added, the excess H+ reacts with the A- to form more HA, lowering the pH and minimising the effect of the addition of acid. When alkali is added, the OH- reacts with the dissociated H+ to form water, which reduces the effect of the alkali by restoring the pH to normal levels. Alka Seltzer acts as a buffer because the citrate ions in solution (C6H5O73-) are able to react with H+ when acid is added, to form citric acid, C6H8O7. This neutralises the acid, increasing the pH. The excess HCO3- ions also react with H+ to form CO2 and water, hence lowering the pH and reducing the acidity of the solution. The HCO3- ions also act as a buffer when alkali is added, because they react with OH- to create water and CO32- ions, increasing the pH and reducing the effect of the addiction of alkali.
The slide was then stained and left to steam with malachite green. It was continuously followed up by applications of the stain so it may remain moist for 10 minutes. The slide was then rinsed and safranin was again used as a counterstain. Using oil immersion objective lens of the microscope, unknown #76 had only reddish-pink cells without any signs of spore formation. Thus the given unknown is a non-spore former.
Different brands contain varied amounts of chemicals and dyes in the candle wax, which could have impact on the result. The experiment should be repeated with another brand to support the
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.