After which the digestions were examined by gel electrophoresis. The samples were run on a 50 mL 0.9% (w/v) agarose gel in 1X TAE buffer at 100 V until the leading track dye traveled 2/3 the distance of the gel. The gel was then soaked in GelRed for 20 minutes and examined under UV light. To prepare the digestions 10 μL of each digestion was mixed with 2 μL of 6X track dye in a micro centrifuge tube. 12 μL of 1 kb DNA ladder and each digestion was run on the
Suspect one’s DNA was inserted into wells four and five; suspect two’s DNA was inserted into wells six and seven. The apparatus was then closed and turned on to run at 100 volts. Electrophoresis ran for 30 minutes; separating the DNA according to size in the gel. Then the tray with the gel was removed and a stain sheet was placed on the gel for 15 minutes. After 15 minutes that gel was rinsed with water and then set in water.
The flow through was discarded. 200 μl of A1 solution was added and centrifuged at 15 000 g for 2 min. The column was then placed in a new micro-centrifuge tube. 25 μl of DNA elution buffer was added directly to the column matrix and incubated at room temperature for 3 min. To elute the DNA, the columns were then transferred to 1.5 ml micro-centrifuge tubes and centrifuged at 15 000 g for 2 min.
PCR product (5 µl) was added to each well and loading dye (5 µl) was added to the final well. The PCR chamber was then sealed and a current of 110mA applied for one hour. The solidified gel was then removed from the chamber and placed under UV light. A digital image was then taken, allowing for analysis to be carried
(1;p 326) Method I collected 1/8 c of saliva, then added the following: 2 drops of dish detergent (stirred 30 sec), 2 drops of contact lens solution (stirred 30 sec), and a small pinch of table salt (stirred 30 sec). Then, I tilted the cup approximately at a 45 degree angle and ran ¼ c of the ice cold rubbing alcohol down the side of the cup, which formed a layer on the top of the saliva-soap solution. (no mixing). Once the solution separated I then took two sticks and wrapped the DNA around them by gently spinning the sticks in the school with the DNA streaks. Final step was mailing the DNA sample to Tory Blackwell for PCR analysis.
Figure 20 and 21 casting tray Figure 22 gel box After 1 hr., take out the gel from gel box carefully, place it into the machine, so that the DNA gel electrophoresis can be visualize under UV light. Figure 23 gel documentation system, used to visualize gel electrophoresis with UV light NMR spectroscopy First of all, 3 samples were prepared, peptide in SDS, DPC, and buffer. The sample temperature was maintained at 298 K, prepared by supervisor and H(hydrogen) in SDS and DPC micelle was replaced with D(deuterium) , so that in proton NMR, peptide won’t be interfered by H in micelle. amount of peptide in sds and dpc In the case of NMR of peptide alone, sample was prepared with 90% of H2O which is 540μL and 10% of D2O which is 60μL, so 600μL of solution was used to dilute 1.5mg of peptide. Put 600μL of sample into NMR Sample Tubes, put the sample tube into NMR sample holder, and then run the test, the chemical shift of proton in peptide can be monitored.
Repeat step four for each sample with a new sterile swab each time. 6. Label the petri dishes according to their samples, and seal each with tape. 7. Then, to take data, each day place the 0.5cm^2 piece of grid paper underneath the petri dish and count the approximate number of bacteria in one
The effect of the solution concentration of sodium chloride on diffusion in yam cores compared to the solution concentration of water Abstract The purpose of the experiment was to see if different solution concentrations had an effect on diffusion. Our group established a hypothesis that stated; sodium chloride will make the yam cores weigh less than in water. In order to start experimenting, we obtained 10 yam cores, weighed them and placed them in five cups that contained 50 mL of water. At 10-minute intervals, we would take them out and weigh them again for 30 minutes. We repeated this experiment using another five set of cups that contained 50 mL of 1M sodium chloride.
While in the room I gave the blood draw specimen box to RN Anglea Wynn. She opened the box and removed the contents. I removed the right cuff from Mr. Defalco’s arm so blood could be taken. She used the betadine in the box to clean the area where the blood would be taken. At 4:29 a.m. blood was drawn, RN Wynn placed the blood in the tubes provided in the box.
The Solid sequencing platform, produced by Technologies/Applied Biosystems (ABI), performs sequencing by ligation method. Similar like the Roche 454 library preparation, genomic double strand DNA were sheared into small pieces and ligated with two types of adatptors P1 and P2 on two ends. One end with P1 adaptor binds onto the surface of the magnetic bead and emulsion PCR takes place to amplify single nucleotide fragment. Then the oil was washed out and four fluorescent labeled di-bases probes were added into the beads mixture. By matching the 1st and 2nd position of the template by di-base probes, fluorescence was detected and the extra tail with fluorescent probe is cleaved out.