Agar Essays

.4 Agar Disk Diffusion The antimicrobial tests were carried out according to disc diffusion tests (Lennette et al., 1985, Kim et al., 1995). Cells were streaked on MHA agar plate to obtain colonies at 37°C for 16 hrs after which they were resuspended in sterile saline solution to give an O.D of 0.1 at 600nm. On solidified 2% MHA plate, the bacterial culture is swabbed and sterile disc impression was made, 10 µl plant extracts was loaded on the agar plate. After 10 minutes the plate is incubated

Bacterium growth on various agar plates Introduction The purpose of this experiment is to show different agar plates inhibit or enable growth of different organisms. Some varieties of media enable the grow of a wide range of organisms such as nutrient agar. Other media are selective which means they contain specific nutrients to encourage the growth of certain organisms. This means other organisms will die due to the selective nutrients such as high concentration of salt which will cause plasmolysis

E. Discussion: In order to synthesize the polymer, Nylon 6,10, we had to complete a few steps to create the chemical reaction that combined sebacoyl chloride and hexamethylenediamine. First we measured the mass of the two graduated cylinders when they were empty, and measured it again after they were filled with sebacoyl chloride and hexamethylenediamine. We did this in order to find the measurements of the reactants. When we measured the graduated cylinder when they were emptied, one weighed at

LB agar was divided into 4 quadrants. First quadrant, which was labeled with C, shows the microbial colonies found on coke. After culturing ,only 1 colony was found on coke. İt has pale yellow colour, circular shape and medium size. 1 colony was seen on money too. It has a same properties with colony found on coke. 2 different types of bacterial colonies were seen on thumb of the washed hand. arrow 4 and 5 indicates the bacterial colonies which were found on washed hand. Arrow 4 from figure 1 shows

concentrations used in Sabouraud’s dextrose agar (SDA). It has ability to produce melanin pigment by using various precursors of melanin in the media. I] Culture Media For primary isolation: - . For primary isolation of cryptococcus bacteriological media like blood agar, chocolate agar and Brain heart infusion agar, Cystein-heart haemoglobin agar, bird seed agar and sunflower seed agar can be used (Chander J, 2009). On Blood and brain Heart Infusion Agar-Cryptococcus grows at 370 C as buff coloured

by Figure 1 does not contain plasmid, there will not be any transformed cells. There is also no antibiotic ampicillin, therefore the cells will not die but they will grow and replicate since it is on the LB agar plate. Therefore on this plate there are only the normal E.coli cells. The LB agar plate is a favorable environment for the bacteria to grow and replicate at its optimum rate (Sezonov, 2007). The plate represented by Figure 2 should not have any bacteria growing on it as it contains the antibiotic

Bacillus thuringiensis was isolated from raw milk (Taif, KSA) on nutrient agar at 37oC for overnight. The supernatant of the bacterial isolate was screened for synthesis of AgNPs. The bacterial isolate was morphologically and biochemically characterized according to Bergy’s Manual of Systemic Bacteriology[21]. Also, this bacterial isolate was further identified by 16S rRNA sequencing. The culture was maintained on a nutrient agar plate/slants at 4°C and as glycerol stocks 40% at −70°C. Preparation of

selecting specific bacteria, I looked for the best combination of variation and choosing across all five of my plates for both agars. Through using morphological observation I was able to determine different bacteria such as colonies that are irregular and circular continuing with variations of elevations and edges. Also, the use of solid cultures such as the use of agar is rewarding because the bacteria will not move creating the ability to have many colonies on the plate while still being isolated

phosphate to ammonia and ammonium hydroxide, which then use to alkalinize the agar. At pH 7.5 or higher, the agar will turn blue. EMB coliform Eosin Methylene Blue Agar is a selective medium because it contains dyes eosin Y and methylene blue, which can inhibit the growth of Gram-positive organisms. Since the unknown bacteria is gram negative, the test is successful as the unknown bacteria grown colonies on the EMB Agar. EMB test is also a differential test because it contains lactose, which encourages

“Post-Lab Questions” What ingredient(s) makes MacConkey agar selective for Gram-negative bacteria? The ingredients that make MacConkey’s Agar selective is a pH indicator (neutral red), and a disaccharide ( Lactose) “2. What types of bacteria are inhibited on MacConkey agar?” Gram-Positive bacteria that are inhibited with MacConkey agar due to crystal violet and bile salt presence . “3. What ingredient(s) makes MacConkey agar differential?” MacConkey agar are inhibited by a pH indicator (neutral red), and

catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one. This is where the antibacterial disc bacitracin was used

streak plate technique to isolate separate colonies. We used a nutrient and MacConkey agar plate to begin. We used both agars because the nutrient agar allows us to grow the largest number of different types of microbes, yet not all bacteria can grow because it is rich with beef broth and some yeast extracts. McConkey agar only allows gram negative bacteria to grow while inhibiting gram positive bacteria. This agar also contains a pH indicator which allows us to detect if fermentation occurred. The

petri dishes were obtained air and skin. The air petri dish lid was left open so airborne bacteria could land in the petri dish. The skin petri dish was divided into four sections for each lab member. We each pressed our fingerprints into the correct agar section to capture bacteria from our skin (Holbrook and Leicht, 2017).

phenospecies test (morphology and biochemistry), based on the protocol of SNI 7303 (2009), plus one control isolate the A. hydrophila ATCC 7699 obtained from Microbiologic Co. Figure 1. The Isolate of A. hydrophila ATCC 7699 Grown on the Rimler-Shoots Agar Medium + Antibiotic Novobiocin at 37C for 24 Hours 3.2 The

Practical Report- Diffusion in Agar Cubes Sabrina Turtur- Stage 1 Biology Introduction Diffusion is the movement of particles (atoms, ions or molecules) from an area in which they are in higher concentration to areas of lower concentration. It continues until the concentration of substances is the same throughout. The act of diffusion occurs in respiration, photosynthesis and osmosis. Without it, cells would not receive the nutrients they need to resume stability. Commonly, molecules found within

Yeast Growth in YPD Agar: To prepare YPD agar, mentioned in Table 1 nutrient ingredients in given concentration were weighed and added to 200 ml of distilled water. The mixture was autoclaved (SMS ASL80 MSV) for 1.5 hours at 121°C. On sterile plates, 25-30 ml of the mixture was poured and left to cool down. The yeast cells were then streaked on the agar plates and the cultures were grown in a stationary incubator (S1-600R, Lab Companion) for 72 hours at 30°C. Yeast Growth in YPD: To prepare YPD liquid

We took 2 Petri plates containing L-agar and spread 50µl from dilutions 〖10〗^(-3) and 〖10〗^(-5) respectively on L-agar plates by using spreader and incubated them at 37°C for 24 hours. After 24 hours incubation, we selected isolated colony and streak it on L-agar containingPetri plates to purify it. After 24 hours incubation, we got our purified colony, now we checked either it is nitrogen fixing

Potato Dextrose Agar 1. 39.0 g of potato dextrose agar powder was dissolved in a litre of sterile distilled water on the hot plate. 2. pH of the solution was adjusted to 5.6 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Plate Count Agar or Total Plate Count 1. 22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot

10x, 20x, 30x, 50x,75x and 100x MIC to each well in 12-well plate. Colony counts (CFU/ml) were determined at different time points (T0, T3, T24) by using appropriate dilutions of each culture. Using spot-plating method (32) 10µl was spotted on LB agar plates. However after 24 hour exposure the bacterial cell from dilutions of ≤ 1x102 were washed twice with sterile PBS prior to plating in triplicate. These were then incubated overnight at 37°C. The experiment for each different density was repeated

Chemistry Exploration Topic: determining the activation energy of a chemical reaction Research Question: What effect does temperature of the chemical reaction have on the activation energy ? ICT: Microsoft Word Autograph Microsoft Excel Introduction This experiment is designed to help in estimating the activation energy of the rate-limiting step in the acid catalyzed reaction of acetone with iodine. This is achieved by measuring the reaction rates at different reaction temperatures over