2.7 Polymerase Chain Reaction (PCR) In 1971, Dr. Har Gobind Khorana’s group described the idea to amplify a DNA (Templeton, 1992). Later on in 1985, Kary B. Mullis invented the Polymerase Chain Reaction (PCR) which is used today in different fields, such as scientific research, clinical diagnostics and criminal investigations. PCR is a molecular biology tool that is used to amplify a fragment of DNA to generate thousands to millions of copies. This technique is based on an enzymatic reaction which
formation and repair of body cells. It forms part of the structure of every cell in your body, including muscle, skin, hair and nails. All the tissues, bones and brain. One of the important protein, collagen helps make scar tissue, forms the protein base of teeth, along with the material of ligaments and tendons and is a strengthening part of arterial walls. It is also needed for making enzymes, hormones and antibodies. Your bodies cannot store protein, so it must be supplied on a daily basis from
Human development happens continuously throughout ones lifespan as we develop and change. Genes exemplify biological factors in human development. Some biological factors are visible, for example skin tone and hair color. Some biological factors are unobserved, for instance genetic abnormalities and risks for diseases. From a biopsychosocial perspective what one becomes is the product of genes, or biological forces. Psychological factors involve effects like coping skills, or temperament. The biopsychosocial
Historical Concept of Lewis acids and bases Gilbert Newton Lewis was one of the great chemists in history. His greatest discovery may well be the theory of the covalent bond in 1916, but he made many other contributions. One was his theory of acids and bases. In 1923, he wrote: "We are so habituated to the use of water as a solvent, and our data are so frequently limited to those obtained in aqueous solutions, that we frequently define an acid or a base as a substance whose aqueous solution gives
titrant) to a known volume of another solution of unknown concentration until the reaction reaches neutralization, which is often indicated by a colour change) with Vinegar as the acid, Phenolphthalein as the indicator and Sodium Hydroxide as the base. Acids and Bases can be defined by three different definitions, the Arrhenius definition, Brønsted-Lowry definition and Lewis definition. The Arrhenius
unknown solutions were bases and which ones were acids. There are many different definitions of acids and bases, such as the Bronsted-Lowry, the Arrhenius, and Lewis definitions. According to The Editors of Encyclopædia Britannica5, a Bronsted-Lowry acid is, “any compound that can transfer a proton to any other compound,” and a base is, “the compound that accepts the proton”. As stated by Librerexts3, “Arrhenius acids form hydrogen ions in aqueous solutions with Arrhenius bases forming hydroxide ions”
Persuasive essay Parents should be permissive Instantly, several parents are struggling to find a way to raise their children effectively. Permissive parenting is one of their choices. Being permissive is treating children with plenty of love, caring and nurturing but not much authorities, rules or punishments. They might make their children lack of self-control or aggressive, but alongside with these disadvantages, there are also numerous benefits. Permissive parenting helps children to be successful
Yes, it does matter if the link is fiber or microwave or some media. The reason for such an assumption is due to the fact the medium’s speed is getting varied. Usually the CAT 5 speed is 100mbps, hence fiber has the highest speed amongst others. Furthermore, Fiber and CAT 5 are full duplex, hence the reason for connecting these two locations, Fast ethernet or Giga bit ethernet ports switches are utilized. The Fast Ethernet specification defines separate physical sub-layers for each media type. Full-duplex
ache, vomiting. Venigar contains a small percentage of ethanoic acid Ch3COOH. This practical aims to find out the concentration of the of the vinegar against a standard solution of sodium hydroxide soloution of concentration 0.1 mol dm3 through acid-base titration, the label on the bottle says 6%. The equation of the reaction between sodium hydroxide and ethanoic acid is as follows: CH3COOH + NaOH → CH3COONa + H2O We can measure the end point of titration process and we can
It is type of condensation reaction, which involve the condensation of acidic anhydride and aldehyde in the presence of weak base (i.e. Sodium and potassium salt of the acid or trimethylamine) to give unsaturated carboxylic acid.(Equation-1)[1].In 1968 Perkin described the very first example of such type condensation reaction, involve the synthesis of coumarin by condensing the sodium or potassium salt of salicylaldehyde with acetic anhydride (Equation-2)[2].Generally such type of reaction is only
Discussion: End point in this acid-base titration experiment refers to the point where the chemical reaction has reached its conclusion and no additional titrant should be added. The end point of this experiment can be obtained when the indicator used changes colour. For example colourless to light pink when phenolphthalein is used and red to orange and subsequently yellow when methyl orange is used. Equivalence point, also known as stoichiometric point in an acid-base titration refers to the point where
There is a lack of education and public information based around dental erosion and furthermore, there is generally widespread ignorance of the deleterious consequences of dental erosion; this is distinctly the issue with erosion due to fruit juices as they’re seen to be healthy. [1][2] Tooth enamel is the hardest substance in the human body and it helps us with things such as chewing and biting. Even though enamel is an extremely solid substance, it can still break, and unlike bone, it cannot repair
Introduction: The acid-base titration experiment is the use of a titrant, an analyte, and an indicator. Titration is the slow addition of one solution of a known concentration (called a titrant) to a known volume of another solution of unknown concentration until the reaction reaches neutralization, which is often indicated by a color change.1 The titrate is what is later released into a beaker or flask that is filled with the analyte and indicator. The color change happens because of the indicator
Introduction Buffer is a solution that resists a change in pH when bases or acid are added. Solutions that are acidic contain high concentrations of hydrogen ions (H+) and have pH values less than seven. Buffer usually consist of a weak acid, and its conjugate base or a weak base and its conjugate acid. The function of buffer is to resist the changes in hydrogen ion concentration as a result of internal and environmental factor. This buffer experiment is important so that we relies the important
The average concentration is surface water is 1.9 µg/L. * Agency for Toxic Substances and Disease Registry (ATSDR). 2002. Toxicological Profile for Beryllium. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service Copper Copper is an essential trace mineral, present in all body tissues, which plays a role in the formation of connective tissue, and in the normal functioning of muscles and the immune and nervous system. copper is necessary to The human body because of copper
Explain the relationship between the ionisation of amino acids and pH |Structural diagram of the neutral structure| |Structural diagram of positively charged structure| |Structural diagram of negatively charged structure| Explain how the form of an amino acid, whether positively charged, negatively charged or neutral, depends of the pH of the solution ? If you increase the pH of a solution of an amino acid by adding hydroxide ions, if this is done then the hydrogen ions will then
The effect of the solution concentration of sodium chloride on diffusion in yam cores compared to the solution concentration of water Abstract The purpose of the experiment was to see if different solution concentrations had an effect on diffusion. Our group established a hypothesis that stated; sodium chloride will make the yam cores weigh less than in water. In order to start experimenting, we obtained 10 yam cores, weighed them and placed them in five cups that contained 50 mL of water. At 10-minute
HUSSAM KANAA HSCL 2415 LAB 5 Bilirubin Rose State College 10/5/2017 Abstract Bilirubin is the breakdown product of hemoglobin, hem, from red blood cells. On average there is 200 to 300 mg of bilirubin is produced every day, and the human body is able to excrete it. Excess amount of bilirubin can be toxic, but low amount of unconjugated hyperbilirubinemia might protect against cardiovascular diseases and tumor development. Bilirubin can help in diagnosing liver and blood
The concentration of the stock solution 2.0x 10-4M as per label information in the lab. However, the calculated volume using the experimental data is 1.5 x 10-4M.There is 25% difference between these concentration caluclated from zero time intercept.The significant difference in the concentration drop happened by many factors.First,the rate ionization is depend the pH because pKa determines the equlibrium between p-nitrophenol and its depronated form p-nitrophenolate.Although,the pH is maintained
to categorize acids and bases into different strengths, following a pH scale. pH stands for “power of Hydrogen”, and is used as a scale in order to conclude whether or not a substance is acidic, basic or neutral. The scale ranges from a pH of 0 to a pH of 14 (with a colour that corresponds with each pH value for colour changing indicators). A substance is an acid if it has a pH level of pH 0 to pH 6. A pH 7 is neutral, which means that it is neither an acid nor a base. A pH of 8 to 14 means