Cytotoxicity Essays

efficiently improved due to the addition of DCMC. The hydrogel fibers are effective biomaterials for using as wound dressings since they can absorb wound exudates and provide moist environment. Moreover, the composite hydrogel fibers present low cytotoxicity. The study indicates that DCMC is an effective crosslinking reagent for biomaterials fixation. The DCMC crosslinked gelatin-PEG composite hydrogel fibers can be well-suited for biomedical applications such as wound

Eugenol at the concentrations of 0.2 -20 µm is suggested to be able to produce a dose dependent and reversible vasodilator response that are partially dependent on the endothelium (24). EUG has also found to have a preventive effect on dopamine depression and lipid peroxidation, which can protect depression induced by 6-hydroxyl dopamine (OHDA). Eugenol has prevented depression by decreasing lipid peroxidation and stimulating reduced glutathione (GSH) may lead to a protecting effect (25)

The problem with these MOFs was they were made of chromium, which was considered to be toxic to be used as a drug delivery vehicle. So another alternative was suggested, Fe-MIL-100 has been developed as a biocompatible drug carrier104.Trimesic acid (BTC or 1,3,5 benzene-tricarboxylic acid) is the organic linker. This cubic mesoporous iron(III)trimesate MIL-100(Fe)105 exhibit a low toxicity character106 raise exceptional interest as drug delivery and bio imaging systems 107. Apart from Cr and Fe,

Tumor treatment by hyperthermia has limitations, however, that the most of nanoparticles do not have high specific absorption rate. At least 10% of tumor weight should be absorbed in order to be effective to heat-ablate tumors through hyperthermia. Treatment of malignant tumors at any site in the body is expected to be possible if agents that convert RF energy into heat can be delivered to the malignant cells. However, RF ablation suffers from the disadvantage that it is an invasive method that often

membrane (PVDF or nitrocellulose) to make them more accessible to the antibodies that specific to the target protein. Cytotoxic assay Cytotoxicity is described as the quality of being toxic to cells. The technique is used to measuring dead cell protease activity, in which they have lost membrane integrity. There are several ways used to measuring cytotoxicity, but generally involved in the assessment of cell membrane integrity. Vital dyes such as propidium iodide or trypan blue can be used to estimate

use. PHB was produced from local isolate Bacillus thuringenesis in a conc. 4.1 g/l using 30 g/L Sugar-cane molasses (SCM) at pH 7.5 and incubation temperature 35oC. PHB biodegradation by soil microorganisms were completed after four weeks. Cell cytotoxicity testing is one of the critical factors affecting the biomedical application of polymers. 50% cell cytotoxic concentration (IC50) = 130 mg/ml while non-toxic concentration was 12.5 mg/ml. studying of direct contact application between biopolymer

The genus Salvia comprises about 900 species distributed all over the world and is one of the major genera belonging to Lamiaceae family (1). Salvia species have been used since ancient times for different purposes, including perfumery industry and for culinary and therapeutic applications. These plants have been traditionally employed for their cerebrovascular and cardiac benefits, anti-inflammatory, antirheumatic, antimicrobial, tranquilizing, anticancer, antidiabetic, hepatoprotective and many

A. Rationale Astaxanthin, similar to a variety of carotenoids, is a colorful antioxidant and a fat-soluble pigment. The presence of the extended polyene system located at the center of the substance causes it to not only give color but also responsible for the antioxidant properties of carotenoids such as astaxanthin that can perform a free radical scavenging and quenching ability (Dalei and Sahoo, 2015). Also, astaxanthin is a very unique carotenoid which cannot be converted into retinol in the

INTRODUCTION Wound infections are one of the bad complications in patients undergoing surgeries. Consequently, infections of different organs or tissues that visible to surgeons may lead to significant increment of postoperative morbidity and mortality beside prolongation of hospital stay.1 It has been well documented that eradication of wound infection resulted in significant increment of patient comfort and decreased medical costs.2 Onche and Adedeji3 stated that Staphylococcus aureus is the predominate

survival and proliferation, also it can aid in quantifying macrophage-mediating cytotoxicity. MTT assay is a highly sensitive and reproductive method, it doesn’t require presence of radioactive compounds, easy to perform and quantify and it’s a rapid method.(Ferrari et al., 1990) A study was conducted on doxorubicin (DOX) and doxorubicin poly(N-isopropylacylamide-co-acrylic aid) (PNA) conjugates to assess their cytotoxicity

Preeclampsia Aetiology Causes of the disease: The cause of preeclampsia is a high blood pressure and proteinuria (5grams or more in 24 hours) in pregnant woman. The exact cause of preeclampsia is not known but it is believed that the causes of preeclampsia is far more than high BP and renal disease but other causes involved such as previous history of preeclampsia, family history of preeclampsia, Chronic hypertension (long term high blood pressure), Liver disease (AST and ALT, ALP as well as low

2. Zinc Oxide Zinc oxide is a white colored inorganic compound which is insoluble in water but is highly soluble in acid or alkaline solutions. It does not occur naturally instead, it is created when zinc is chemically heated and combined with oxygen molecules. There are two possible structural forms of zinc oxide: hexagonal and cubic. Hexagonal crystals are commonly found in nature. With the advent of nanotechnology, nano-engineered Zinc oxide is commercially used in various cosmetic products because

2.2.1 Preparation of sPEG from polyoxyethylene (20) sorbitanmonolaurate (tween-20) Star shaped polyoxyethylene (sPEG) was synthesized according to the literature with some changes [18]. Briefly, 8 g of polyoxyethylene (20) sorbitan monostearatev were dissolved in 20 ml of THF in a round bottom flask and then 1 g of KOH was added as hydrolysis agent. After refluxing about 24 h, the solution was concentrated and added to a mixture of acidic water/hexane (1 : 1). The aqueous phase in which the sPEG

Double Trouble was an orange tabby cat whose life was full of Trouble. She was a gentle creature and should have been allowed to live the normal life of a cat. However, this was not to be. She was one of several cats at University of Wisconsin-Madison (UW) that were cruelly experimented on. These experiments caused her to develop infections, begin twitching, become depressed, and even cause half her face to be paralyzed. The experiments that were done on them were inhumane, and animal experimentation

Throughout history, progressive mental deterioration in old age, has been recognized and described. However, not until 1906 did Dr. Alois Alzheimer, a German physician, identify abnormalities in the brain cells of his patient, Auguste D (52 year old), as a disease. Auguste D’s brain autopsy was performed after she died of severe memory problems, confusion and difficulty in understanding questions. The autopsy revealed the nerve cells surrounded by dense deposits (neuritic plaques). The inside of

Habit of smokeless tobacco (SLT) as ‘‘chewing tobacco’’ or ‘‘spit tobacco’’ becoming a global threat for human health day by day due to its numerous deleterious effects. The usage of SLT was reported to be more prevalent in South Asian countries comparing to the Western world [1], although current studies had revealed the world wide usage of SLT-related products [2, 3]. Chewing tobacco or spit tobacco, which is largely used in India and also in USA, is mixed with betel leaves, areca nut, lime and

Polyunsaturated fatty acids are well known for its protective effect on human health. However, they contain multiple double bonds which are susceptible to lipid oxidation. These have been long recognized and it is the major challenge in oil industry. Lipid oxidation is the main factor of food deterioration. It not only affects fatty acid composition and the nutritional quality of the food, it also causes undesirable changes in colour, aroma and produce toxic compounds (Wasowicz, 2004). The toxic

Here, with a mature BBB, the effect of MSG has a different mechanism; MSG-induced cytotoxicity was associated with increased oxidative stress and led to apoptosis and autophagy of pancreatic β- cells (Di Cairano et al., 2011). It has been reported that MSG has neurotoxic effects resulting in brain cell damage, retinal degeneration, endocrine

Definition Chemical carcinogenesis is commonly defined to indicate the initiation or development of neoplasia by chemicals. It comprises not only epithelial malignancies (carcinomas) but also mesenchymal malignant tumors (sarcomas). People can be exposed to carcinogens which are present in food, air, or water. In addition, some carcinogens are created within the body as products of metabolism or certain pathophysiologic states like inflammation or oxidative stress. Carcinogens from the environment

Burn wound size reduction By measuring the burn wound area before and after definite intervals of time, reduction in burn wound defect area was calculated using equation. At 3rd day there was no reduction in burn wound injury in all the groups. At 7th day there was not much reduction in burn wound for group A (control) and B (conventional marketed cream). There was 77% reduction in burn wound of group D at 7th day post-wounding. At 10th day post-wounding, burn wound healing started leading to about